Sect 6 Quiz Easy

Choose a study mode

Play Quiz
Study Flashcards
Spaced Repetition
Chat to Lesson

Podcast

Play an AI-generated podcast conversation about this lesson

Questions and Answers

What is the effect of histone deacetylases (HDACs) on chromatin?

  • They promote condensation of chromatin. (correct)
  • They promote decondensation of chromatin.
  • They enhance transcriptional activity.
  • They interfere with DNA replication.

Which type of chromatin is characterized by being highly condensed?

  • Heterochromatin (correct)
  • Active chromatin
  • Facultative chromatin
  • Euchromatin

What role does HP1 play in chromatin structure?

  • It prevents chromatin condensation.
  • It binds to trimethylated lysine 9 on histone H3. (correct)
  • It promotes deacetylation of histones.
  • It inhibits histone methylation.

What is associated with transcriptionally active chromatin?

<p>Bromodomains (D)</p> Signup and view all the answers

What does the term 'histone code' refer to?

<p>The pattern of histone tail modifications. (A)</p> Signup and view all the answers

Which kind of DNA modification is not part of epigenetics?

<p>Alterations to the genetic code (B)</p> Signup and view all the answers

How does boundary element function in chromatin?

<p>By blocking histone methylation on one side. (A)</p> Signup and view all the answers

What modification is characteristic of euchromatin?

<p>Hyperacetylation (D)</p> Signup and view all the answers

What is the function of the left LTR in the retroviral life cycle?

<p>Serves as a promoter for transcription (A)</p> Signup and view all the answers

Which enzyme synthesizes the dsDNA in retroviral replication?

<p>Reverse transcriptase (B)</p> Signup and view all the answers

What are endogenous retroviruses (ERVs) primarily comprised of?

<p>Full-length proviral DNA (C)</p> Signup and view all the answers

What type of DNA comprises approximately 6% of the human genome?

<p>Simple-sequence DNA (B)</p> Signup and view all the answers

Which of the following statements about nonviral retrotransposons is true?

<p>They lack LTRs and are therefore called nonviral retrotransposons. (A)</p> Signup and view all the answers

In the human genome, what percentage of genomic DNA is transcribed into pre-mRNA precursors?

<p>50% (D)</p> Signup and view all the answers

What percentage of the human genome is comprised of LINEs?

<p>21% (A)</p> Signup and view all the answers

Which of the following describes the length range of introns in human DNA?

<p>90 bp to several kilobases (A)</p> Signup and view all the answers

Which LINE is the most common element found in the human genome?

<p>L1 LINE (C)</p> Signup and view all the answers

What is the significance of the A/T rich region in LINEs?

<p>It is important for retrotransposition. (D)</p> Signup and view all the answers

What is the typical length range of human exons?

<p>50 to 200 bp (D)</p> Signup and view all the answers

What type of DNA consists of short sequences repeated in long tandem arrays and is found at specific chromosome locations?

<p>Satellite DNA (D)</p> Signup and view all the answers

How many copies of SINEs are typically found in the human genome?

<p>1.6 million copies (C)</p> Signup and view all the answers

Which of the following types of DNA is known for its variations in length and includes segments that are often many kilobases long?

<p>Introns (D)</p> Signup and view all the answers

What is the primary reason for the lack of a consistent relationship between DNA amount and phylogenetic complexity among eukaryotes?

<p>The presence of nonfunctional DNA (A)</p> Signup and view all the answers

What is commonly implicated in neuromuscular diseases due to expanded segments?

<p>Microsatellites (D)</p> Signup and view all the answers

In mammals, where do the majority of mitochondria in the embryo come from?

<p>Virtually all from the egg (D)</p> Signup and view all the answers

What is the inheritance pattern of mitochondrial DNA in mice?

<p>99.99% maternal and 0.01% paternal (B)</p> Signup and view all the answers

What causes the degenerative optic nerve condition in Leber's hereditary optic neuropathy?

<p>A missense mutation in NADH-CoQ reductase (B)</p> Signup and view all the answers

What is heteroplasmy in the context of mtDNA?

<p>A mixture of wild-type and mutant mtDNA in a cell (C)</p> Signup and view all the answers

Which of the following describes petite mitochondria?

<p>They result from a deletion in mtDNA affecting oxidative phosphorylation. (D)</p> Signup and view all the answers

Which statement is true about mitochondrial genetic codes?

<p>Animal and fungal mtDNA codes differ from that of bacteria. (A)</p> Signup and view all the answers

What mainly encodes proteins in chloroplasts?

<p>Circular cpDNA (C)</p> Signup and view all the answers

What is the typical size range of cpDNA in chloroplasts?

<p>Approximately 120-160 kb (D)</p> Signup and view all the answers

What is a gene defined as in terms of nucleic acid sequence?

<p>The complete sequence needed for synthesis of a functional gene product. (B)</p> Signup and view all the answers

Which of the following is NOT a component of nuclear eukaryotic DNA?

<p>Ribosomal RNA (C)</p> Signup and view all the answers

What does the term 'histone code' refer to?

<p>The set of epigenetic modifications on histone proteins. (B)</p> Signup and view all the answers

Which aspect of chromatin structure is primarily affected by histone tail modifications?

<p>The physical structure and accessibility of chromatin. (D)</p> Signup and view all the answers

Which of the following best contrasts prokaryotic and eukaryotic gene structure?

<p>Eukaryotic genes typically contain both exons and introns, while prokaryotic genes rarely do. (B)</p> Signup and view all the answers

Which classes of DNA are found in eukaryotic nuclei?

<p>Euchromatin and heterochromatin. (B)</p> Signup and view all the answers

What role do transposons play in DNA?

<p>They can move within the genome and affect gene expression. (C)</p> Signup and view all the answers

Which of these statements regarding organelle DNA is true?

<p>Organelle DNA is exclusively circular. (B)</p> Signup and view all the answers

What is the primary function of the coding region of a gene?

<p>To code for the amino acid or RNA sequence. (C)</p> Signup and view all the answers

In prokaryotes, what characterizes an operon?

<p>It produces polycistronic mRNAs for related functions. (B)</p> Signup and view all the answers

What is a key characteristic of eukaryotic genes compared to prokaryotic genes?

<p>Eukaryotic genes contain lengthy introns and noncoding regions. (B)</p> Signup and view all the answers

What role do splice sites play in complex transcriptional units in eukaryotic genes?

<p>They allow for the production of different mRNAs from the same gene. (D)</p> Signup and view all the answers

What is one major difference between monocistronic and polycistronic mRNAs?

<p>Monocistronic mRNAs are more common in eukaryotes. (A)</p> Signup and view all the answers

Which of the following regions in eukaryotic genes often requires processing to create multiple mRNA variants?

<p>Polyadenylation site. (A)</p> Signup and view all the answers

Which of the following components are commonly found in eukaryotic genes but absent in prokaryotic genes?

<p>Intron sequences. (B)</p> Signup and view all the answers

What is the transcriptional-control region primarily responsible for?

<p>Regulating the expression of a gene. (D)</p> Signup and view all the answers

Flashcards

Histone tails

The 'tails' of histone proteins, which extend out from the core histone octamer and can be chemically modified.

Heterochromatin

Highly condensed chromatin, usually inactive.

Euchromatin

Less condensed chromatin, generally active in transcription.

Histone Deacetylases (HDACs)

Enzymes that remove acetyl groups from histone tails, promoting chromatin condensation.

Signup and view all the flashcards

Histone methylation

Adding methyl groups to histone tails, impacting chromatin structure and gene activity.

Signup and view all the flashcards

Chromatin condensation

The process of packaging DNA to form more compact structures.

Signup and view all the flashcards

Chromodomain

A protein domain that binds to methylated histone tails.

Signup and view all the flashcards

Heterochromatin Protein 1 (HP1)

A protein that binds to methylated histone H3 (Lysine 9), promoting heterochromatin formation.

Signup and view all the flashcards

Boundary elements

Regions in chromosomes where non-histone proteins block heterochromatin spreading.

Signup and view all the flashcards

Bromodomains

Protein domains that recognize and bind to acetylated histone tails.

Signup and view all the flashcards

Epigenetics

The study of changes in gene expression caused by modifications, not changes in the DNA sequence.

Signup and view all the flashcards

Histone code

The specific combination of chemical modifications (methylation, acetylation) on histone tails that influence gene expression.

Signup and view all the flashcards

Gene

The entire nucleic acid sequence needed to make a functional gene product (protein or RNA).

Signup and view all the flashcards

Prokaryotic Gene Structure

Genes in prokaryotes are typically continuous/uninterrupted.

Signup and view all the flashcards

Eukaryotic Gene Structure

Genes in eukaryotes often have non-coding regions (introns) within them

Signup and view all the flashcards

Coding DNA

Parts of a gene with the sequence of nucleotides needed to construct the proteins

Signup and view all the flashcards

Noncoding DNA

DNA sequence that does not code for protein.

Signup and view all the flashcards

Exons

Coding regions within a eukaryote gene.

Signup and view all the flashcards

Introns

Non-coding regions within a eukaryote gene.

Signup and view all the flashcards

Chromatin

The complex of DNA, proteins, and other molecules that make up chromosomes.

Signup and view all the flashcards

Chromosomes

Structures carrying genetic information in cells.

Signup and view all the flashcards

Histone Code

The specific combination of modifications (methylation, acetylation).

Signup and view all the flashcards

Genomics

Study of genomes of different species.

Signup and view all the flashcards

Paralogs

Genes related by duplication within the same species.

Signup and view all the flashcards

Orthologs

Genes in different species that evolved from a common ancestor.

Signup and view all the flashcards

Epigenetics

Heritable changes in gene expression without changes to the DNA sequence.

Signup and view all the flashcards

Gene definition

The complete DNA sequence needed to produce a functional RNA or protein.

Signup and view all the flashcards

Coding region

Part of the gene that codes for amino acid sequence in proteins.

Signup and view all the flashcards

Transcriptional control region

Region in gene that controls the process of making RNA from DNA.

Signup and view all the flashcards

Prokaryotic Gene Structure

Prokaryotic genes are continuous, without introns.

Signup and view all the flashcards

Eukaryotic Gene Structure

Eukaryotic Genes include introns (non-coding regions) interrupting the coding sequence.

Signup and view all the flashcards

Operon

A single transcriptional unit (in prokaryotes) that encodes multiple proteins.

Signup and view all the flashcards

Monocistronic mRNA

mRNA that directs the synthesis of a single polypeptide chain.

Signup and view all the flashcards

Polycistronic mRNA

mRNA that encodes multiple polypeptide chains from a single transcript.

Signup and view all the flashcards

Introns

Non-coding regions within a eukaryotic gene.

Signup and view all the flashcards

Exons

Coding regions within a eukaryotic gene.

Signup and view all the flashcards

Noncoding DNA

DNA sequences that do not code for proteins.

Signup and view all the flashcards

Eukaryotic DNA variation

The amount of DNA doesn't always match evolutionary relationships (phylogeny) among eukaryotes.

Signup and view all the flashcards

Exon length

Eukaryotic protein-coding regions (exons) are typically 50-200 base pairs long, except the 3' end, which are often longer.

Signup and view all the flashcards

Intron length

Eukaryotic non-coding regions (introns) have varying lengths, with a median of 3.3 kilobases.

Signup and view all the flashcards

Pre-mRNA processing

About half of human genomic DNA is transcribed into pre-mRNA, but 95% of those sequences reside in introns, which get removed during splicing.

Signup and view all the flashcards

Repetitious DNA

Eukaryotic DNA contains repetitive sequences besides protein-coding genes.

Signup and view all the flashcards

Simple-sequence DNA

6% of the human genome, composed of short, repeated sequences.

Signup and view all the flashcards

Satellite DNA

Short DNA sequences (1-500 bp) repeated in long tandem arrays, often found in centromeres and telomeres.

Signup and view all the flashcards

Microsatellites

A type of satellite DNA with repeats of 1-13 base pairs.

Signup and view all the flashcards

Expanded microsatellites

Microsatellites that have become larger (due to daughter-strand slippage) and linked to diseases.

Signup and view all the flashcards

Mitochondrial Inheritance

In most animals, mitochondria are inherited solely from the mother.

Signup and view all the flashcards

mtDNA

Mitochondrial DNA; a small circular DNA molecule present in mitochondria.

Signup and view all the flashcards

Heteroplasmy

A cell containing a mixture of both normal and mutated mitochondrial DNA.

Signup and view all the flashcards

Leber's Hereditary Optic Neuropathy

A disease caused by mutations in mtDNA, leading to optic nerve degeneration and eventual blindness.

Signup and view all the flashcards

Cytoplasmic Inheritance

Traits passed down through the cytoplasm, often exclusively from one parent (e.g. mother's mitochondria).

Signup and view all the flashcards

Petite Mitochondria

Smaller mitochondria with defective oxidative phosphorylation, often due to mtDNA deletions.

Signup and view all the flashcards

Chloroplast DNA (cpDNA)

Circular DNA found in chloroplasts, encoding proteins mainly for photosynthesis.

Signup and view all the flashcards

Photosynthesis

The process by which plants and other organisms convert light energy into chemical energy.

Signup and view all the flashcards

Retroviral LTR Promoter

The left LTR controls transcription initiation at the 5' end of the retroviral RNA genome, using host RNA polymerase II.

Signup and view all the flashcards

Retroviral LTR Processing

The right LTR directs the addition of a poly(A) tail to the 3'end of the viral RNA genome by host cell enzymes.

Signup and view all the flashcards

Retroviral Reverse Transcription

Reverse transcriptase converts retroviral RNA into double-stranded DNA (dsDNA) with complete LTRs.

Signup and view all the flashcards

Retroviral Integration

Integrase inserts the retroviral dsDNA into the host cell genome.

Signup and view all the flashcards

Endogenous Retrovirus (ERV)

Common LTR sequences in humans derived from full-length proviral DNA, where internal retroviral sequences were deleted through recombination.

Signup and view all the flashcards

Nonviral Retrotransposons

Mobile genetic elements lacking LTRs like LINEs and SINEs.

Signup and view all the flashcards

Long Interspersed Elements (LINEs)

Abundant mobile elements (e.g., L1, L2, L3) in mammals with the enzyme reverse transcriptase and a crucial A/T rich region.

Signup and view all the flashcards

Short Interspersed Elements (SINEs)

Second most abundant type of mobile elements (~ 13% human DNA), typically 300bp long, and do not encode proteins.

Signup and view all the flashcards

L1 Retrotransposons

The most prevalent LINE in the human genome with ORF1 coding for an RNA-binding protein and ORF2 coding for reverse transcriptase and DNA endonuclease.

Signup and view all the flashcards

Study Notes

Genes, Chromatin, and Chromosomes

  • A gene is the complete nucleic acid sequence needed for a functional gene product (polypeptide or RNA).
  • Eukaryotic genes contain coding regions (for amino acids/RNA) and transcriptional control regions (promoters).
  • Prokaryotic genes often occur in operons, which are single transcriptional units for related functions.
  • Eukaryotic genes are typically monocistronic, meaning one mRNA codes for one protein. They also frequently contain introns.
  • The human genome is approximately 96% similar to the genomes of the greater apes.

Eukaryotic Gene Structure

  • Genes are DNA containing regions coding for RNA molecules (e.g., tRNA, rRNA).
  • Eukaryotic genes have coding regions interspersed with non-coding DNA; genes may contain many non-coding DNA regions.
  • Eukaryotic genes contain exons (coding regions) and introns (non-coding regions).
  • Introns are removed during mRNA processing.
  • Different forms of Transcriptional units yield various mRNAs from a single gene.

Prokaryotic Genes

  • Prokaryotic genes often produce polycistronic mRNAs, meaning one mRNA can code for multiple proteins.
  • Prokaryotic mRNAs lack introns.
  • Operons control gene expression in prokaryotes.

Gene Families

  • Gene families are sets of duplicated genes with similar sequences.
  • Paralogs are genes within the same species that arose from duplication events.
  • Orthologs are genes in different species that arose from a common ancestor.
  • Duplicated genes arose via gene duplication.

Noncoding DNA

  • Much of eukaryotic genomes consists of non-coding DNA.
  • Intergenic regions are stretches of DNA between genes.
  • Simple-sequence DNA is composed of repeated short sequences.
  • Repetitive DNA is a significant portion of eukaryotic genomes.

Transposable Elements

  • Transposable elements, or transposons, are DNA sequences that can change position within a genome.
  • Two types of transposons: DNA transposons and retrotransposons.
  • DNA transposons move directly as DNA.
  • Retrotransposons are transcribed into RNA, then reverse transcribed to DNA before moving.
  • Transposons sometimes cause mutations.
  • LINEs and SINEs are types of retrotransposons.

Chromosomal Organization of Genes

  • Density of genes varies across a chromosome.
  • Human exon sequences comprise about 2% of the genome.
  • Gene copying events produce similar genes.
  • Intergenic regions often contain regulatory elements.

Variation Between Individuals

  • Single nucleotide polymorphisms (SNPs) account for ~1-2% of genomic differences between diverse human individuals.
  • These variations frequently occur in non-coding regions.
  • Deletion and duplication events alter gene copy numbers.

Eukaryotic Chromosomes

  • Chromosomes are composed of chromatin, which is a complex of DNA and proteins (histones).
  • Nucleosomes are DNA-protein complexes with a characteristic “beads-on-a-string” structure.
  • Histone tails are subject to modifications (e.g., methylation, acetylation).
  • Euchromatin is less condensed, while heterochromatin is more compact.
  • Interphase chromosomes are less tightly compacted.
  • Metaphase chromosomes are most tightly compacted (typically observed in microscopic cells).
  • Nonhistone proteins constitute up to half of the chromosomal mass.
  • Chromosome structures are organized by banding (e.g., Giemsa staining).

Genomics

  • Genomics is the study of complete genomes.
  • Genomic analyses include comparative genome sequencing and analysis, and identifying and characterizing genes.
  • Databases, such as GenBank for sequence information, are important resources in genomics.

Organelle DNAs

  • Mitochondrial DNA and chloroplast DNA have different characteristics.
  • Mitochondrial DNA (mtDNA) has non-coding regions.
  • mtDNA is involved in energy production
  • mtDNA is inherited primarily from the mother.
  • Chloroplast DNAs are often circular.

Studying That Suits You

Use AI to generate personalized quizzes and flashcards to suit your learning preferences.

Quiz Team

Related Documents

More Like This

Use Quizgecko on...
Browser
Browser