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General Chromatography Techniques

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Questions and Answers

What is the primary purpose of thin-layer chromatography (TLC)?

To separate proteins based on their molecular weight
To separate ions based on their charge
To separate different organic chemicals based on their polarity (correct)
To separate DNA fragments based on their size

Which of the following is NOT a requirement for the mobile phase (solvent system) in TLC?

High purity
Stability
Low viscosity
High boiling point (correct)

What is the purpose of the stationary phase in column chromatography?

To provide a polar or non-polar surface for separation (correct)
To act as the solvent system
To elute the sample components
To separate the sample components based on their size

In paper chromatography, which of the following statements is TRUE?

Polar compounds move faster than non-polar compounds (A) Signup and view all the answers

What is the primary function of the mobile phase in column chromatography?

To elute the sample components from the stationary phase (C) Signup and view all the answers

Which of the following statements about mobile phase in chromatography is correct?

It consists of the sample being separated and the solvent that moves the sample through the stationary phase. (A) Signup and view all the answers

What is the primary cause of separation of components in chromatography?

The difference in the interaction of components with the stationary phase. (B) Signup and view all the answers

Which of the following statements about the stationary phase in chromatography is correct?

It can be a solid, a gel, or a liquid. (A) Signup and view all the answers

What is the primary difference between preparative and analytical chromatography?

Preparative chromatography is used for separation, while analytical chromatography is used for identification and quantitation. (C) Signup and view all the answers

Which of the following is NOT a type of chromatography used in qualitative and quantitative analyses, according to the given text?

Gas chromatography (D) Signup and view all the answers