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Questions and Answers
What is the main purpose of chromatography?
Who coined the term chromatography?
What was the first analytical use of chromatography described for?
What factor affects the separation process in chromatography related to liquid-solid interaction?
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What does the mobile phase do in the process of chromatography?
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What is the mobile phase composed of in chromatography?
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What is the basis of the chromatography technique?
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What are some applications of chromatography in the pharmaceutical sector?
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What does HPLC and GC detect in the chemical industry?
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In which industry is chromatography used for forensic pathology and crime scene testing?
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What type of interaction is the basic component effective on the separation of molecules in chromatography?
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Which technique uses mechanisms of ion exchange, surface adsorption, partition, and size exclusion?
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In which industry are HPLC and GC used for detecting various contaminants such as polychlorinated biphenyl (PCBs) in pesticides and oils?
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What is the main purpose of chromatography in the chemical industry?
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What is the basis of the chromatography technique involving various characteristics such as size and shape, total charge, hydrophobic groups, and binding capacity with the stationary phase?
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What is the role of the stationary phase in chromatography?
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Which molecular characteristics affect the separation process in chromatography related to liquid-solid interaction?
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What was the first analytical use of chromatography described for?
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In which year was the term 'chromatography' coined?
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What is the main purpose of chromatography?
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Study Notes
- Chromatography is a biophysical technique used for separating, identifying, and purifying components of mixtures for qualitative and quantitative analysis.
- Mikhail Tswett coined the term chromatography in 1906. The first analytical use was described by James and Martin in 1952 for gas chromatography of fatty acid mixtures.
- Chromatography relies on molecular differences to separate materials, using a stationary phase and a mobile phase.
- The stationary phase is a solid or a liquid adsorbed on a solid support. The mobile phase is a liquid or gas.
- Substances interact with the stationary and mobile phases, leading to different chromatography techniques based on interaction types and stationary bed methods.
- Four separation techniques based on molecular characteristics and interaction type use mechanisms of ion exchange, surface adsorption, partition, and size exclusion.
- Applications of chromatography include:
- Pharmaceutical sector: identifying and analyzing samples, separation of compounds, and drug development.
- Chemical industry: testing water and air samples, detecting contaminants, and analyzing materials.
- Food industry: food spoilage and additive detection, determining nutritional quality.
- Forensic science: analyzing evidence in crime scenes and forensic pathology.
- Molecular biology: studying metabolomics, proteomics, and nucleic acid research, protein separation techniques.
- Chromatography is used extensively across various industries due to its powerful separation capabilities, especially for complex mixtures.
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Description
Test your knowledge about chromatography, the biophysical technique used for separating, identifying, and purifying components of mixtures for analysis. Learn about the history, techniques, and applications of chromatography.
