Gene Structure and Transcription Quiz

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Questions and Answers

Which of the following components is NOT a part of a nucleotide?

  • Deoxyribose sugar
  • Nitrogenous base
  • Amino acid (correct)
  • Phosphate group

The DNA double helix is primarily stabilized by:

  • Ionic interactions between phosphate groups
  • Covalent bonds between sugar molecules
  • Phosphodiester bonds between nucleotides
  • Hydrogen bonds between complementary bases (correct)

Which type of RNA molecule carries the genetic information from DNA to the ribosome for protein synthesis?

  • snRNA
  • tRNA
  • mRNA (correct)
  • rRNA

The region of a gene where RNA polymerase initially binds to initiate transcription is called the:

<p>Promoter (D)</p> Signup and view all the answers

Introns are:

<p>Non-coding sequences that are transcribed but removed during RNA processing (B)</p> Signup and view all the answers

Which RNA polymerase is responsible for synthesizing mRNA in eukaryotic cells?

<p>RNA Polymerase II (D)</p> Signup and view all the answers

Transcription factors are:

<p>Proteins that bind to DNA and regulate the rate of transcription (C)</p> Signup and view all the answers

The mediator complex plays a crucial role in:

<p>Transcription initiation (A)</p> Signup and view all the answers

The unwinding of the DNA double helix during transcription creates a:

<p>Transcription bubble (B)</p> Signup and view all the answers

In prokaryotic transcription, the sigma factor is essential for:

<p>Promoter recognition and binding of RNA polymerase (A)</p> Signup and view all the answers

Rho-dependent and Rho-independent mechanisms are involved in:

<p>Transcription termination in prokaryotes (D)</p> Signup and view all the answers

In eukaryotic transcription, the polyadenylation signal sequence is important for:

<p>Termination of transcription and addition of a poly(A) tail (B)</p> Signup and view all the answers

Which of the following base pairs in DNA is held together by three hydrogen bonds?

<p>Guanine (G) and Cytosine (C) (C)</p> Signup and view all the answers

The directionality of DNA and RNA synthesis always proceeds in the:

<p>5' to 3' direction (D)</p> Signup and view all the answers

Which of the following statements about RNA polymerase is INCORRECT?

<p>It requires a primer to initiate RNA synthesis. (A)</p> Signup and view all the answers

The genetic code is said to be degenerate because:

<p>Multiple codons can code for the same amino acid (B)</p> Signup and view all the answers

Which of the following is NOT a characteristic of eukaryotic gene expression?

<p>Transcription and translation occur simultaneously (D)</p> Signup and view all the answers

The process of removing introns and joining exons together during RNA processing is called:

<p>RNA splicing (B)</p> Signup and view all the answers

Which of the following statements about transcription factors is TRUE?

<p>They can either enhance or repress transcription. (C)</p> Signup and view all the answers

The TATA box is a common:

<p>Promoter element in eukaryotes (C)</p> Signup and view all the answers

Enhancers are DNA sequences that:

<p>Increase the rate of transcription (D)</p> Signup and view all the answers

Which of the following processes does NOT occur in the nucleus of eukaryotic cells?

<p>Translation (D)</p> Signup and view all the answers

The poly(A) tail added to the 3' end of eukaryotic mRNA molecules:

<p>All of the above (D)</p> Signup and view all the answers

Which of the following is a key difference between prokaryotic and eukaryotic transcription?

<p>Prokaryotic transcription and translation can occur simultaneously, while eukaryotic transcription occurs in the nucleus and translation occurs in the cytoplasm. (B)</p> Signup and view all the answers

What is the primary function of a gene?

<p>To store genetic information for building and maintaining an organism.</p> Signup and view all the answers

What is the difference between introns and exons?

<p>Introns are transcribed but not translated, while exons are both transcribed and translated.</p> Signup and view all the answers

What is the purpose of splicing during RNA processing?

<p>To remove introns and join exons, creating the mature mRNA.</p> Signup and view all the answers

Which enzyme is responsible for synthesizing mRNA from DNA during transcription?

<p>RNA polymerase</p> Signup and view all the answers

Where does transcription take place in eukaryotic cells?

<p>Nucleus</p> Signup and view all the answers

What is the role of mRNA in protein synthesis?

<p>It carries the genetic information from DNA to the ribosomes.</p> Signup and view all the answers

Flashcards

Nucleotide component NOT in DNA

Amino acid is not a component of a nucleotide in DNA. Nucleotides consist of a sugar, phosphate, and a nitrogenous base.

DNA double helix stabilization

Hydrogen bonds between complementary DNA bases (A-T, G-C) hold the double helix together.

mRNA function

mRNA carries genetic information from DNA to ribosomes for protein synthesis.

Promoter region

Region of a gene where RNA polymerase binds to start transcription.

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Introns

Non-coding DNA sequences within a gene that are removed during RNA processing.

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mRNA synthesis enzyme (eukaryotes)

RNA polymerase II synthesizes mRNA in eukaryotic cells.

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Transcription factors

Proteins that bind to DNA and control the rate of transcription.

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Mediator complex function

Plays a vital role in the initiation of transcription by interacting with other proteins and RNA polymerase.

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Transcription bubble

Unwinding of the DNA double helix during transcription.

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Sigma factor (prokaryotes)

Essential for promoter recognition and RNA polymerase binding in prokaryotic transcription.

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Prokaryotic transcription termination

Methods like Rho-dependent and Rho-independent mechanisms terminate transcription.

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Polyadenylation signal (eukaryotes)

Important for transcription termination and poly(A) tail addition in eukaryotes.

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3 hydrogen bond DNA base pair

Guanine (G) and Cytosine (C) form a base pair held together by three hydrogen bonds.

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Transcription/Replication direction

RNA and DNA synthesis proceed in the 5' to 3' direction

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RNA polymerase primer requirement

RNA polymerase does not require a primer to begin RNA synthesis unlike DNA polymerase.

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Degenerate genetic code

Multiple codons can code for the same amino acid

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Eukaryotic gene expression characteristic

Transcription and translation happen separately in eukaryotic cells

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RNA splicing

Process of removing introns and joining exons in RNA

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Study Notes

Gene Structure and Transcription

  • Nucleotide components: Deoxyribose sugar, phosphate group, and nitrogenous base. Amino acid is not a nucleotide component.
  • DNA double helix stabilization: Primarily hydrogen bonds between complementary bases.
  • RNA molecule for genetic information transfer: mRNA carries genetic information from DNA to ribosomes for protein synthesis.
  • Transcription initiation region: Promoter region where RNA polymerase binds.
  • Introns: Non-coding sequences removed from RNA during processing.
  • RNA polymerase II: Responsible for mRNA synthesis in eukaryotes.
  • Transcription factors: Proteins that bind to DNA, regulating transcription rates. They can either enhance or repress transcription.
  • Transcription bubble: Unwinding of DNA double helix during transcription.
  • Genetic code degeneracy: Multiple codons can code for the same amino acid.
  • Eukaryotic gene expression characteristics: Presence of introns, mRNA processing (capping, splicing, polyadenylation), and regulation of gene expression at multiple levels.
  • RNA splicing: Removing introns and joining exons in RNA processing to create mature mRNA.
  • Prokaryotic vs. Eukaryotic transcription differences: Prokaryotes can simultaneously transcribe and translate, while eukaryotes transcribe in the nucleus and translate in the cytoplasm. Prokaryotic mRNA undergoes less extensive processing than eukaryotic mRNA. They utilize different RNA polymerases.
  • Poly(A) tail function: Added to the 3' end of eukaryotic mRNA to protect from degradation and facilitate transport out of the nucleus.
  • Promoter element (TATA box): A DNA sequence crucial for transcription initiation in eukaryotes.
  • Enhancers: DNA sequences that increase transcription rates by enhancing the binding of RNA polymerase.

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