Gene Cloning
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Questions and Answers

What is the purpose of the strong promoter in an expression vector?

  • To inhibit transcription of the gene of interest
  • To enhance translation of the gene of interest
  • To drive high-level transcription of the gene of interest (correct)
  • To prevent translation of the gene of interest
  • Why is a transcription termination sequence necessary in an expression vector?

  • To initiate transcription of the gene of interest
  • To stop transcription at the appropriate point (correct)
  • To stabilize the plasmid vector
  • To enhance transcription of the gene of interest
  • What is the role of the antibiotic resistance sequences in the expression vector?

  • To enhance the expression of the gene of interest
  • To allow for selection of transformed cells containing the plasmid (correct)
  • To inhibit the growth of transformed cells
  • To facilitate the insertion of the gene of interest
  • Why is the blue/white screening method used in identifying recombinant plasmids?

    <p>To distinguish between plasmids with and without the gene of interest</p> Signup and view all the answers

    What is the purpose of the radioactive DNA probe in the identification of recombinant plasmids?

    <p>To specifically bind to the gene of interest</p> Signup and view all the answers

    Why is a ribosomal binding site and start codon necessary in an expression vector?

    <p>To initiate translation of the gene of interest</p> Signup and view all the answers

    What is the function of the polylinker in an expression vector?

    <p>To facilitate the insertion of the gene of interest</p> Signup and view all the answers

    Why are transformed bacterial cells grown on medium containing ampicillin during the identification process?

    <p>To select for cells containing the plasmid</p> Signup and view all the answers

    What is gene cloning?

    <p>Insertion of foreign DNA into a replicating cell to produce many copies of the DNA</p> Signup and view all the answers

    What is the purpose of Southern blot technique in gene cloning?

    <p>To locate a specific gene of interest</p> Signup and view all the answers

    What is the function of an expression vector in gene cloning?

    <p>To introduce a specific gene into a target cell for protein expression</p> Signup and view all the answers

    What is the purpose of using a plasmid in gene cloning?

    <p>To act as an expression vector for protein production</p> Signup and view all the answers

    What is the role of regulatory sequences in an expression vector?

    <p>To enhance and promote efficient transcription and translation of the gene</p> Signup and view all the answers

    What is the first step in the gene cloning process?

    <p>Extract and purify DNA from cells</p> Signup and view all the answers

    What is the purpose of cutting DNA with restriction enzymes in gene cloning?

    <p>To obtain DNA fragments for further manipulation</p> Signup and view all the answers

    What is the function of Agarose gel electrophoresis in gene cloning?

    <p>To separate DNA fragments based on size</p> Signup and view all the answers

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