Gene Cloning

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16 Questions

What is the purpose of the strong promoter in an expression vector?

To drive high-level transcription of the gene of interest

Why is a transcription termination sequence necessary in an expression vector?

To stop transcription at the appropriate point

What is the role of the antibiotic resistance sequences in the expression vector?

To allow for selection of transformed cells containing the plasmid

Why is the blue/white screening method used in identifying recombinant plasmids?

To distinguish between plasmids with and without the gene of interest

What is the purpose of the radioactive DNA probe in the identification of recombinant plasmids?

To specifically bind to the gene of interest

Why is a ribosomal binding site and start codon necessary in an expression vector?

To initiate translation of the gene of interest

What is the function of the polylinker in an expression vector?

To facilitate the insertion of the gene of interest

Why are transformed bacterial cells grown on medium containing ampicillin during the identification process?

To select for cells containing the plasmid

What is gene cloning?

Insertion of foreign DNA into a replicating cell to produce many copies of the DNA

What is the purpose of Southern blot technique in gene cloning?

To locate a specific gene of interest

What is the function of an expression vector in gene cloning?

To introduce a specific gene into a target cell for protein expression

What is the purpose of using a plasmid in gene cloning?

To act as an expression vector for protein production

What is the role of regulatory sequences in an expression vector?

To enhance and promote efficient transcription and translation of the gene

What is the first step in the gene cloning process?

Extract and purify DNA from cells

What is the purpose of cutting DNA with restriction enzymes in gene cloning?

To obtain DNA fragments for further manipulation

What is the function of Agarose gel electrophoresis in gene cloning?

To separate DNA fragments based on size

Test your knowledge on gene cloning with this quiz! Learn about the elements and steps involved in gene cloning, including insertion, transformation, and identification. See how much you know about the process of introducing foreign DNA into replicating cells and amplifying DNA fragments.

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