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Questions and Answers
What is the purpose of the strong promoter in an expression vector?
What is the purpose of the strong promoter in an expression vector?
Why is a transcription termination sequence necessary in an expression vector?
Why is a transcription termination sequence necessary in an expression vector?
What is the role of the antibiotic resistance sequences in the expression vector?
What is the role of the antibiotic resistance sequences in the expression vector?
Why is the blue/white screening method used in identifying recombinant plasmids?
Why is the blue/white screening method used in identifying recombinant plasmids?
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What is the purpose of the radioactive DNA probe in the identification of recombinant plasmids?
What is the purpose of the radioactive DNA probe in the identification of recombinant plasmids?
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Why is a ribosomal binding site and start codon necessary in an expression vector?
Why is a ribosomal binding site and start codon necessary in an expression vector?
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What is the function of the polylinker in an expression vector?
What is the function of the polylinker in an expression vector?
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Why are transformed bacterial cells grown on medium containing ampicillin during the identification process?
Why are transformed bacterial cells grown on medium containing ampicillin during the identification process?
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What is gene cloning?
What is gene cloning?
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What is the purpose of Southern blot technique in gene cloning?
What is the purpose of Southern blot technique in gene cloning?
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What is the function of an expression vector in gene cloning?
What is the function of an expression vector in gene cloning?
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What is the purpose of using a plasmid in gene cloning?
What is the purpose of using a plasmid in gene cloning?
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What is the role of regulatory sequences in an expression vector?
What is the role of regulatory sequences in an expression vector?
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What is the first step in the gene cloning process?
What is the first step in the gene cloning process?
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What is the purpose of cutting DNA with restriction enzymes in gene cloning?
What is the purpose of cutting DNA with restriction enzymes in gene cloning?
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What is the function of Agarose gel electrophoresis in gene cloning?
What is the function of Agarose gel electrophoresis in gene cloning?
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