Podcast
Questions and Answers
What is the purpose of gel electrophoresis in DNA testing?
What is the purpose of gel electrophoresis in DNA testing?
- To extract DNA from cells
- To identify specific nucleotide patterns in DNA
- To amplify DNA samples
- To separate DNA fragments based on their size (correct)
What is the function of a restriction enzyme in DNA testing?
What is the function of a restriction enzyme in DNA testing?
- To cut DNA at specific nucleotide patterns (correct)
- To amplify DNA samples
- To visualize DNA bands on a gel
- To separate DNA fragments based on their size
What is the purpose of polymerase chain reaction (PCR) in DNA testing?
What is the purpose of polymerase chain reaction (PCR) in DNA testing?
- To make many copies of a DNA sample (correct)
- To visualize DNA bands on a gel
- To separate DNA fragments based on their size
- To identify specific nucleotide patterns in DNA
What property of DNA fragments causes small fragments to move through the gel faster than large ones in gel electrophoresis?
What property of DNA fragments causes small fragments to move through the gel faster than large ones in gel electrophoresis?
What happens to small DNA fragments when they are run through the gel?
What happens to small DNA fragments when they are run through the gel?
What is the purpose of loading DNA samples into wells at one end of a gel?
What is the purpose of loading DNA samples into wells at one end of a gel?
What happens when electricity is run through the DNA gel in the testing process?
What happens when electricity is run through the DNA gel in the testing process?
Why is it necessary to make many copies of a DNA sample in the PCR process?
Why is it necessary to make many copies of a DNA sample in the PCR process?
What does the presence of DNA bands in the gel indicate?
What does the presence of DNA bands in the gel indicate?
What is the function of a DNA-binding dye in the gel staining process?
What is the function of a DNA-binding dye in the gel staining process?
What is the main principle behind gel electrophoresis?
What is the main principle behind gel electrophoresis?
How are DNA fragments loaded onto the gel in gel electrophoresis?
How are DNA fragments loaded onto the gel in gel electrophoresis?
What is the purpose of running electricity through the gel in gel electrophoresis?
What is the purpose of running electricity through the gel in gel electrophoresis?
Which DNA fragments move faster through the gel in gel electrophoresis?
Which DNA fragments move faster through the gel in gel electrophoresis?
What are the practical applications of gel electrophoresis?
What are the practical applications of gel electrophoresis?
In crime scene DNA analysis, what samples can be compared to identify suspects?
In crime scene DNA analysis, what samples can be compared to identify suspects?
What do paternity tests compare to determine biological relationships?
What do paternity tests compare to determine biological relationships?
Apart from crime solving and paternity tests, what else can gel electrophoresis be used for?
Apart from crime solving and paternity tests, what else can gel electrophoresis be used for?
How can gel electrophoresis results help in studying genetic relationships?
How can gel electrophoresis results help in studying genetic relationships?
What is the significance of gel electrophoresis in DNA analysis?
What is the significance of gel electrophoresis in DNA analysis?
Flashcards
Gel electrophoresis purpose
Gel electrophoresis purpose
Separates DNA fragments based on their size.
Restriction enzyme function
Restriction enzyme function
Cuts DNA at specific nucleotide patterns.
PCR purpose
PCR purpose
To make many copies of a DNA sample.
Fragment mobility factor
Fragment mobility factor
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Small fragments in gel
Small fragments in gel
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DNA sample loading
DNA sample loading
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Electricity in DNA gel
Electricity in DNA gel
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PCR necessity
PCR necessity
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DNA bands indication
DNA bands indication
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DNA-binding dye function
DNA-binding dye function
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Gel electrophoresis principle
Gel electrophoresis principle
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DNA loading location
DNA loading location
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Electricity purpose
Electricity purpose
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Faster DNA fragments
Faster DNA fragments
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Gel electrophoresis applications
Gel electrophoresis applications
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Crime scene analysis samples
Crime scene analysis samples
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Paternity tests comparison
Paternity tests comparison
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Gel electrophoresis use
Gel electrophoresis use
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Genetic relationships study
Genetic relationships study
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Gel electrophoresis significance
Gel electrophoresis significance
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Study Notes
Understanding Gel Electrophoresis
- Gel electrophoresis separates DNA fragments by size
- Restriction enzymes cut DNA at specific sequences
- DNA fragments are loaded onto one side of a gel
- Electricity is run through the gel to separate DNA fragments
- Smaller DNA fragments move faster through the gel
- Gel electrophoresis can be used to solve crimes and paternity tests
- Crime scene DNA samples can be compared to victim, suspect, and control samples
- Paternity tests compare child's DNA with suspected father and mother samples
- Gel electrophoresis can also study evolutionary relationships
- Evolutionary relationships can be determined by comparing DNA samples of different species
- Gel electrophoresis results can show closeness of genetic relationships
- Gel electrophoresis is a key technique in DNA analysis
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