Gel Electrophoresis

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Questions and Answers

What is the purpose of gel electrophoresis in DNA testing?

  • To extract DNA from cells
  • To identify specific nucleotide patterns in DNA
  • To amplify DNA samples
  • To separate DNA fragments based on their size (correct)

What is the function of a restriction enzyme in DNA testing?

  • To cut DNA at specific nucleotide patterns (correct)
  • To amplify DNA samples
  • To visualize DNA bands on a gel
  • To separate DNA fragments based on their size

What is the purpose of polymerase chain reaction (PCR) in DNA testing?

  • To make many copies of a DNA sample (correct)
  • To visualize DNA bands on a gel
  • To separate DNA fragments based on their size
  • To identify specific nucleotide patterns in DNA

What property of DNA fragments causes small fragments to move through the gel faster than large ones in gel electrophoresis?

<p>Size (C)</p> Signup and view all the answers

What happens to small DNA fragments when they are run through the gel?

<p>They move faster through the gel (A)</p> Signup and view all the answers

What is the purpose of loading DNA samples into wells at one end of a gel?

<p>To prepare the DNA for running through the gel (C)</p> Signup and view all the answers

What happens when electricity is run through the DNA gel in the testing process?

<p>DNA fragments move towards the positive electrode (D)</p> Signup and view all the answers

Why is it necessary to make many copies of a DNA sample in the PCR process?

<p>To have enough DNA for analysis (C)</p> Signup and view all the answers

What does the presence of DNA bands in the gel indicate?

<p>Groups of same-sized DNA fragments (C)</p> Signup and view all the answers

What is the function of a DNA-binding dye in the gel staining process?

<p>To visualize DNA bands in the gel (B)</p> Signup and view all the answers

What is the main principle behind gel electrophoresis?

<p>Separating DNA fragments by size (B)</p> Signup and view all the answers

How are DNA fragments loaded onto the gel in gel electrophoresis?

<p>One side of the gel (A)</p> Signup and view all the answers

What is the purpose of running electricity through the gel in gel electrophoresis?

<p>To separate DNA fragments (B)</p> Signup and view all the answers

Which DNA fragments move faster through the gel in gel electrophoresis?

<p>Smaller DNA fragments (A)</p> Signup and view all the answers

What are the practical applications of gel electrophoresis?

<p>Solving crimes and paternity tests (C)</p> Signup and view all the answers

In crime scene DNA analysis, what samples can be compared to identify suspects?

<p>Victim, suspect, and control samples (D)</p> Signup and view all the answers

What do paternity tests compare to determine biological relationships?

<p>Child's DNA with suspected father and mother samples (B)</p> Signup and view all the answers

Apart from crime solving and paternity tests, what else can gel electrophoresis be used for?

<p>Studying evolutionary relationships (D)</p> Signup and view all the answers

How can gel electrophoresis results help in studying genetic relationships?

<p>Show closeness of genetic relationships (C)</p> Signup and view all the answers

What is the significance of gel electrophoresis in DNA analysis?

<p>It is a key technique in DNA analysis (C)</p> Signup and view all the answers

Flashcards

Gel electrophoresis purpose

Separates DNA fragments based on their size.

Restriction enzyme function

Cuts DNA at specific nucleotide patterns.

PCR purpose

To make many copies of a DNA sample.

Fragment mobility factor

Size

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Small fragments in gel

They move faster through the gel.

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DNA sample loading

To prepare the DNA for running through the gel.

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Electricity in DNA gel

DNA fragments move towards the positive electrode.

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PCR necessity

To have enough DNA for analysis.

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DNA bands indication

Groups of same-sized DNA fragments.

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DNA-binding dye function

To visualize DNA bands in the gel.

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Gel electrophoresis principle

Separating DNA fragments by size.

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DNA loading location

One side of the gel.

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Electricity purpose

To separate DNA fragments.

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Faster DNA fragments

Smaller DNA fragments.

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Gel electrophoresis applications

Solving crimes and paternity tests.

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Crime scene analysis samples

Victim, suspect, and control samples.

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Paternity tests comparison

Child's DNA with suspected father and mother samples.

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Gel electrophoresis use

Studying evolutionary relationships.

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Genetic relationships study

Show closeness of genetic relationships

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Gel electrophoresis significance

It is a key technique in DNA analysis.

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Study Notes

Understanding Gel Electrophoresis

  • Gel electrophoresis separates DNA fragments by size
  • Restriction enzymes cut DNA at specific sequences
  • DNA fragments are loaded onto one side of a gel
  • Electricity is run through the gel to separate DNA fragments
  • Smaller DNA fragments move faster through the gel
  • Gel electrophoresis can be used to solve crimes and paternity tests
  • Crime scene DNA samples can be compared to victim, suspect, and control samples
  • Paternity tests compare child's DNA with suspected father and mother samples
  • Gel electrophoresis can also study evolutionary relationships
  • Evolutionary relationships can be determined by comparing DNA samples of different species
  • Gel electrophoresis results can show closeness of genetic relationships
  • Gel electrophoresis is a key technique in DNA analysis

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