Gel Electrophoresis Quiz: Test Your Knowledge

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Questions and Answers

What is the purpose of gel electrophoresis in DNA testing?

To extract DNA from cells

To identify specific nucleotide patterns in DNA

To amplify DNA samples

To separate DNA fragments based on their size (correct)

What is the function of a restriction enzyme in DNA testing?

To cut DNA at specific nucleotide patterns (correct)

To amplify DNA samples

To visualize DNA bands on a gel

To separate DNA fragments based on their size

What is the purpose of polymerase chain reaction (PCR) in DNA testing?

To make many copies of a DNA sample (correct)

To visualize DNA bands on a gel

To separate DNA fragments based on their size

To identify specific nucleotide patterns in DNA

What property of DNA fragments causes small fragments to move through the gel faster than large ones in gel electrophoresis?

Size Signup and view all the answers

What happens to small DNA fragments when they are run through the gel?

They move faster through the gel Signup and view all the answers

What is the purpose of loading DNA samples into wells at one end of a gel?

To prepare the DNA for running through the gel Signup and view all the answers

What happens when electricity is run through the DNA gel in the testing process?

DNA fragments move towards the positive electrode Signup and view all the answers

Why is it necessary to make many copies of a DNA sample in the PCR process?

To have enough DNA for analysis Signup and view all the answers

What does the presence of DNA bands in the gel indicate?

Groups of same-sized DNA fragments Signup and view all the answers

What is the function of a DNA-binding dye in the gel staining process?

To visualize DNA bands in the gel Signup and view all the answers

What is the main principle behind gel electrophoresis?

Separating DNA fragments by size Signup and view all the answers

How are DNA fragments loaded onto the gel in gel electrophoresis?

One side of the gel Signup and view all the answers

What is the purpose of running electricity through the gel in gel electrophoresis?

To separate DNA fragments Signup and view all the answers

Which DNA fragments move faster through the gel in gel electrophoresis?

Smaller DNA fragments Signup and view all the answers

What are the practical applications of gel electrophoresis?

Solving crimes and paternity tests Signup and view all the answers

In crime scene DNA analysis, what samples can be compared to identify suspects?

Victim, suspect, and control samples Signup and view all the answers

What do paternity tests compare to determine biological relationships?

Child's DNA with suspected father and mother samples Signup and view all the answers

Apart from crime solving and paternity tests, what else can gel electrophoresis be used for?

Studying evolutionary relationships Signup and view all the answers

How can gel electrophoresis results help in studying genetic relationships?

Show closeness of genetic relationships Signup and view all the answers

What is the significance of gel electrophoresis in DNA analysis?

It is a key technique in DNA analysis Signup and view all the answers

Study Notes

Understanding Gel Electrophoresis

Gel electrophoresis separates DNA fragments by size
Restriction enzymes cut DNA at specific sequences
DNA fragments are loaded onto one side of a gel
Electricity is run through the gel to separate DNA fragments
Smaller DNA fragments move faster through the gel
Gel electrophoresis can be used to solve crimes and paternity tests
Crime scene DNA samples can be compared to victim, suspect, and control samples
Paternity tests compare child's DNA with suspected father and mother samples
Gel electrophoresis can also study evolutionary relationships
Evolutionary relationships can be determined by comparing DNA samples of different species
Gel electrophoresis results can show closeness of genetic relationships
Gel electrophoresis is a key technique in DNA analysis

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Description

Test your knowledge of gel electrophoresis with this quiz! Learn about how DNA fragments are separated by size, the use of restriction enzymes, and the applications of gel electrophoresis in crime solving, paternity tests, and studying evolutionary relationships.