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DNA Profile Analysis Using PCR Amplification and Agarose Gel Electrophoresis
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DNA Profile Analysis Using PCR Amplification and Agarose Gel Electrophoresis

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Questions and Answers

What would the presence of three differently sized bands resulting from one PCR reaction in this experiment suggest?

  • The PCR reaction was not successful
  • The DNA sample was contaminated
  • The individual is homozygous at the D1S58 locus
  • The individual is heterozygous at the D1S58 locus (correct)

    • The sizes of the PCR products of the D1S80 locus in one family were 531 and 643 bp in the mother and 435 and 531 bp in the father. What are all possible fingerprints their children could have?

  • 531 bp and 643 bp
  • 435 bp and 643 bp
  • 435 bp and 531 bp
  • All of the above (correct)

    • Assume that a PCR product containing one 16 bp repeat unit is 383 bp, a PCR product containing two 16 bp repeat units is 399 bp, etc. If the sizes of the PCR products are 399 bp and 415 bp, how many 16 bp repeat units are present in each product?

  • 2 and 3 (correct)
  • 4 and 5
  • 3 and 4
  • 5 and 6

    • Based on the information provided about the pMCT118 locus, which of the following is true?

    <p>This protocol alone could not be used to link a suspect with a crime or establish a paternity relationship</p> Signup and view all the answers

    From the results on your gel, if you appear to have the MseI+ haplotype, what does this suggest?

    <p>You are homozygous for the MseI+ allele</p> Signup and view all the answers

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