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Molecular Biology: Gel Electrophoresis Basics

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Questions and Answers

What is the main purpose of gel electrophoresis in DNA analysis?

To neutralize the charge of DNA fragments
To cut DNA fragments into smaller pieces
To separate DNA fragments based on size (correct)
To replicate DNA within bacterial cells

Which type of gel is most commonly used in gel electrophoresis for DNA separation?

Polyacrylamide
Silica
Cellulose
Agarose (correct)

What visual marker is used to observe DNA fragments after gel electrophoresis?

Fluorescent dyes
Radiolabeled probes
Ethidium bromide (correct)
Agarose crystals

What defines a cloning vector like a plasmid or bacteriophage?

<p>Their high copy number within bacterial cells (A)</p> Signup and view all the answers

How do smaller DNA fragments behave in gel electrophoresis compared to larger fragments?

<p>They migrate farther than larger fragments (B)</p> Signup and view all the answers

Flashcards

Gel Electrophoresis

A technique used to separate DNA fragments based on size.

Cloning Vector

A DNA molecule used to carry foreign DNA into a host cell for replication.

DNA Fragment Separation

DNA pieces are separated by size using gel electrophoresis.

Agarose Gel

A common gel medium used in gel electrophoresis to separate DNA fragments.

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Restriction Enzyme

Enzyme that cuts DNA at specific sequences.