Gel Electrophoresis: Types of Buffers and Stains

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What is the primary reason for adding glycerol to the gel?

To improve the gel's ability to separate DNA molecules
To facilitate the gel's solidification
To increase the buffer's viscosity (correct)
To enhance the heating process in the microwave

What is the purpose of adding ethidium bromide to the gel?

To provide a visual indicator for DNA presence (correct)
To facilitate the heating process in the microwave
To increase the gel's viscosity
To enhance the gel's ability to separate DNA molecules

What is the primary application of DNA extraction in forensic investigations?

To determine optimal therapeutic approaches
To determine identity or guide criminal investigations (correct)
To study DNA structures and understand genetics
To identify gene mutations

What is the name of the technique used to study DNA structures and understand genetics?

Genetic Research (D) Signup and view all the answers

What is the purpose of the gel electrophoresis unit?

To inject the samples and separate DNA molecules (C) Signup and view all the answers

What is the ratio of ethidium bromide to the gel?

1:2 microliters (C) Signup and view all the answers

What is the purpose of using a buffer in the gel mixture?

To provide a stable pH environment for the gel (C) Signup and view all the answers

What is the application of DNA extraction in genetic diagnosis?

To identify gene mutations and for early diagnosis of genetic diseases (A) Signup and view all the answers

What is the name of the technique used to determine optimal therapeutic approaches based on genetically identified diseases?

Personalized Medicine (C) Signup and view all the answers

What is the purpose of imaging the gel using UV light?

To provide a visual indicator for DNA presence (D) Signup and view all the answers

Why add glycerol to gel?

Glycerol is a viscous liquid used to increase the density of the gel, making it harder and more stable. This prevents the gel from melting or collapsing during electrophoresis.

What does ethidium bromide do?

Ethidium bromide is a fluorescent dye that binds to DNA and glows under UV light, making DNA visible. This allows researchers to see where the DNA bands are located in the gel.

Forensic DNA extraction purpose?

DNA extraction in forensics helps determine the identity of individuals involved in criminal cases. It can link suspects to crime scenes by comparing DNA samples.