Gel Electrophoresis Technique

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Questions and Answers

What is the primary purpose of gel electrophoresis in laboratories?

  • To synthesize macromolecules
  • To analyze the structure of nucleic acids
  • To separate macromolecules based on their size (correct)
  • To study the electrical properties of proteins

Which of the following molecules can be separated using gel electrophoresis?

  • Hormones and vitamins
  • Enzymes and antibiotics
  • Proteins and nucleic acids (correct)
  • Carbohydrates and lipids

What drives the movement of macromolecules through the gel in gel electrophoresis?

  • Electrical field (correct)
  • Centrifugal force
  • Magnetic field
  • Gravity

What is the basis of the separation of macromolecules in gel electrophoresis?

<p>Rate of movement through the gel (C)</p> Signup and view all the answers

What is the type of substance that is typically separated using gel electrophoresis?

<p>Macro-molecules (B)</p> Signup and view all the answers

What type of field is used to influence the movement of macromolecules through the gel in gel electrophoresis?

<p>an electrical field</p> Signup and view all the answers

What is the common characteristic of the substances separated using gel electrophoresis?

<p>They are macromolecules</p> Signup and view all the answers

What determines the rate of movement of a molecule through the gel in gel electrophoresis?

<p>The rate of movement is determined by the molecule's size and charge</p> Signup and view all the answers

What is the main purpose of the gel in gel electrophoresis?

<p>To provide a sieving medium for the molecules to move through</p> Signup and view all the answers

What type of molecules are typically analyzed using gel electrophoresis?

<p>Biological macromolecules, such as proteins and nucleic acids</p> Signup and view all the answers

Flashcards

Gel Electrophoresis Purpose

Separates macromolecules based on size.

Molecules Separated

Proteins and nucleic acids.

Driving Force

An electrical field.

Basis of Separation

Rate of movement through the gel.

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Type of Substance Separated

Macromolecules.

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Rate of Movement Factors

Molecule's size and charge.

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Main Purpose of Gel

To provide a sieving medium.

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Analyzed Molecules

Biological macromolecules, like proteins and nucleic acids.

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Study Notes

Gel Electrophoresis

  • A laboratory technique used to separate macromolecules, such as proteins and nucleic acids.
  • Separation is based on the rate of movement of these molecules through a gel.
  • The movement is influenced by an electrical field.
  • The gel acts as a barrier, allowing smaller molecules to move faster than larger ones.
  • The technique is useful for separating and identifying specific molecules within a mixture.

Gel Electrophoresis

  • A laboratory technique used to separate macromolecules, such as proteins and nucleic acids.
  • Separation is based on the rate of movement of these molecules through a gel.
  • The movement is influenced by an electrical field.
  • The gel acts as a barrier, allowing smaller molecules to move faster than larger ones.
  • The technique is useful for separating and identifying specific molecules within a mixture.

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