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Questions and Answers
What is the primary purpose of gel electrophoresis in a laboratory?
What is the primary purpose of gel electrophoresis in a laboratory?
- To study the behavior of macromolecules in a non-electrical field
- To synthesize macromolecules
- To analyze the structure of small molecules
- To separate macromolecules based on their size and charge (correct)
What type of molecules are typically separated using gel electrophoresis?
What type of molecules are typically separated using gel electrophoresis?
- Organic molecules, such as carbohydrates and proteins
- Micromolecules, such as lipids and hormones
- Macromolecules, such as proteins and nucleic acids (correct)
- Small molecules, such as sugars and amino acids
What is the driving force behind the separation of macromolecules in gel electrophoresis?
What is the driving force behind the separation of macromolecules in gel electrophoresis?
- Electrical field (correct)
- Magnetic field
- Centrifugal force
- Gravity
What is the basis of the separation of macromolecules in gel electrophoresis?
What is the basis of the separation of macromolecules in gel electrophoresis?
What is the role of the gel in gel electrophoresis?
What is the role of the gel in gel electrophoresis?
What determines the direction of electrophoresis?
What determines the direction of electrophoresis?
Which of the following factors affects the speed of electrophoresis?
Which of the following factors affects the speed of electrophoresis?
What is the role of the supporting medium in electrophoresis?
What is the role of the supporting medium in electrophoresis?
Which factor does not affect the direction of electrophoresis?
Which factor does not affect the direction of electrophoresis?
What is the effect of increasing the temperature of operation on electrophoresis?
What is the effect of increasing the temperature of operation on electrophoresis?
Study Notes
Gel Electrophoresis
- A laboratory technique used to separate macromolecules, specifically proteins and nucleic acids.
- Separation is based on the rate of movement through a gel.
- The movement is influenced by an electrical field.
- This technique is effective for separating macromolecules of varying sizes and charges.
Electrophoresis Factors
- Direction of electrophoresis depends on the net charge of the molecule
- Size and shape of the molecule influence the speed of electrophoresis
- Electric field strength affects the speed of electrophoresis
- The nature of the supporting medium impacts the electrophoresis process
- Temperature of operation influences the electrophoresis outcome
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Description
Gel electrophoresis is a laboratory technique used to separate macromolecules such as proteins and nucleic acids based on their rate of movement through a gel under an electrical field.
