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Gel Electrophoresis Technique

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@InstrumentalBalalaika

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Questions and Answers

What is the primary purpose of gel electrophoresis in a laboratory?

To study the behavior of macromolecules in a non-electrical field

To synthesize macromolecules

To analyze the structure of small molecules

To separate macromolecules based on their size and charge (correct)

What type of molecules are typically separated using gel electrophoresis?

Organic molecules, such as carbohydrates and proteins

Micromolecules, such as lipids and hormones

Macromolecules, such as proteins and nucleic acids (correct)

Small molecules, such as sugars and amino acids

What is the driving force behind the separation of macromolecules in gel electrophoresis?

Electrical field (correct)

Magnetic field

Centrifugal force

Gravity

What is the basis of the separation of macromolecules in gel electrophoresis?

Their rate of movement through a gel under an electrical field Signup and view all the answers

What is the role of the gel in gel electrophoresis?

To provide a medium for the separation of macromolecules Signup and view all the answers

What determines the direction of electrophoresis?

Net charge of the molecule Signup and view all the answers

Which of the following factors affects the speed of electrophoresis?

Electric field strength Signup and view all the answers

What is the role of the supporting medium in electrophoresis?

To influence the speed of electrophoresis Signup and view all the answers

Which factor does not affect the direction of electrophoresis?

Temperature of operation Signup and view all the answers

What is the effect of increasing the temperature of operation on electrophoresis?

It increases the speed of electrophoresis Signup and view all the answers

Study Notes

Gel Electrophoresis

A laboratory technique used to separate macromolecules, specifically proteins and nucleic acids.
Separation is based on the rate of movement through a gel.
The movement is influenced by an electrical field.
This technique is effective for separating macromolecules of varying sizes and charges.

Electrophoresis Factors

Direction of electrophoresis depends on the net charge of the molecule
Size and shape of the molecule influence the speed of electrophoresis
Electric field strength affects the speed of electrophoresis
The nature of the supporting medium impacts the electrophoresis process
Temperature of operation influences the electrophoresis outcome

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Description

Gel electrophoresis is a laboratory technique used to separate macromolecules such as proteins and nucleic acids based on their rate of movement through a gel under an electrical field.