Podcast
Questions and Answers
Which stain is most commonly used in the direct analysis of fungi?
Which stain is most commonly used in the direct analysis of fungi?
Which of the following statements about KOH stain is NOT true?
Which of the following statements about KOH stain is NOT true?
Which dye uses negative staining to highlight Cryptococcus neoformans in the cerebrospinal fluid?
Which dye uses negative staining to highlight Cryptococcus neoformans in the cerebrospinal fluid?
What brightener dye is used as a whitening agent in fluorescing cellulose and chitin?
What brightener dye is used as a whitening agent in fluorescing cellulose and chitin?
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Which media is used for converting dimorphic fungus Blastomyces dermatitidis from its mold to yeast form?
Which media is used for converting dimorphic fungus Blastomyces dermatitidis from its mold to yeast form?
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Which diagnostic method is used for examining secretion and detecting malignant cells?
Which diagnostic method is used for examining secretion and detecting malignant cells?
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Which medium is used for the recovery and differentiation of all Aspergillus species highlighted in green mossy color?
Which medium is used for the recovery and differentiation of all Aspergillus species highlighted in green mossy color?
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What is the derived version of Sabouraud’s Dextrose Agar known as 'Mycobiotic' used for?
What is the derived version of Sabouraud’s Dextrose Agar known as 'Mycobiotic' used for?
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What is the standard culture medium for isolation of fungal agents?
What is the standard culture medium for isolation of fungal agents?
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What is the dilution result after adding a minimum of 5 ml blood to the broth medium?
What is the dilution result after adding a minimum of 5 ml blood to the broth medium?
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Which of the following is a histochemical stain used for detecting glycogen in fungi?
Which of the following is a histochemical stain used for detecting glycogen in fungi?
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Which of the following statements regarding CSF collection is false?
Which of the following statements regarding CSF collection is false?
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Which component is not typically found in a fungal cell wall?
Which component is not typically found in a fungal cell wall?
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Study Notes
Diagnostic Mycology
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KOH Stain: Most commonly used stain for direct analysis of fungi.
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Histochemical Stains for Fungi:
- Methenamine Silver: Highlights glycogen.
- Periodic Acid Schiff: Used to detect certain fungal elements.
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KOH Stain Properties:
- Clears fungal cell walls; alkali-sensitive.
- Gentle heating can accelerate the reaction.
- Used for direct examination of fungi.
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Negative Staining in Fungal Diagnosis: India Ink is used to highlight Cryptococcus neoformans in cerebrospinal fluid (CSF).
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Whitening Agents: Calcofluor White is a brightener used for fluorescing cellulose and chitin.
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Malignant Cell Detection: Papanicolaou Stain is used for examining secretion in the detection of malignant cells.
Differential Media
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Conversion Media for Dimorphic Fungi:
- Cottonseed Conversion Agar converts Blastomyces dermatitidis from mold to yeast form.
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Aspergillus Differentiation Media:
- Czapek’s Agar highlights all Aspergillus species in green mossy color.
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Standard Isolation Culture for Fungi:
- Sabouraud’s Dextrose Agar is the standard for isolating fungal agents.
Aspergillus in Food Preservation
- Preservative Component: Aspergillus species utilize lactic acid in the preservation of bread.
Procedures and Conditions
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Blood Dilution in Broth Medium: Adding a minimum of 5 ml blood to broth results in a dilution of 1:10 to 1:20.
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CSF Collection: Must be collected aseptically via lumbar puncture, processed immediately, and not stored at room temperature.
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Storage Temperatures:
- Hair, Skin Scrapings, and Tissue can be stored at room temperature.
- Sputum must not be stored at room temperature.
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Fungal Cell Wall Components: Fungal cell walls typically contain chitin and ergosterol; however, peptidoglycan is not present.
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Description
Test your knowledge on the techniques and media used in diagnostic mycology. This quiz covers various staining methods, including KOH and histochemical stains, as well as differential media for identifying dimorphic fungi. Perfect for students and professionals in microbiology and medical mycology.
