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Questions and Answers
What is the role of sucrose in Czapexsolex media?
What is the role of sucrose in Czapexsolex media?
Why is potassium important for fungal growth?
Why is potassium important for fungal growth?
What typically happens to fungal growth diameter when a component is removed from the media?
What typically happens to fungal growth diameter when a component is removed from the media?
How are the nutrients in Czapexsolex media classified?
How are the nutrients in Czapexsolex media classified?
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What is the effect of temperature on fungal growth as observed in the experiment?
What is the effect of temperature on fungal growth as observed in the experiment?
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What occurs when magnesium is removed from the media?
What occurs when magnesium is removed from the media?
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Which component provides a source for protein and enzyme synthesis?
Which component provides a source for protein and enzyme synthesis?
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Which of the following statements about the procedure for preparing Czapexdax agar media is correct?
Which of the following statements about the procedure for preparing Czapexdax agar media is correct?
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Study Notes
Section 2: Essential Nutrient Components for Fungal Growth
- Czapek Dox Media: Contains sucrose, sodium nitrate (source of N), sulfuric acid (source of S), potassium sulfate (source of K), ferrous sulfate (source of Fe)
Procedure for Fungal Growth
- Flask Preparation: One flask contains all components, others lack certain components. The flask with all components exhibits the best fungal growth.
- Sterilization: Sterilize the media in an autoclave at 121°C for 15-20 minutes.
- Cooling: Allow the sterilized media to cool.
- Pouring into plates: Pour the cooled media into pre-sterilized petri dishes.
- Solidification: Allow the media to solidify at room temperature.
- Inoculation: Introduce tested fungal specimens onto the plates.
- Incubation: Incubate the plates at 30°C for 7 days.
- Measurement: Measure the diameter of fungal growth.
Observation - Fungal Growth Comparison (Page 2)
- Fungal growth diameter decreases when comparing to the first plate/flask.
- Nutrient classification:
- Macro nutrients (e.g., C, N, S, P, K): Needed in large amounts by fungi.
- Micro nutrients (e.g., Cu, Co, Ca, Zn, Fe): Needed in small amounts by fungi.
Potassium (K+)
- Enzyme Activation: Potassium is a co-factor for enzyme activity.
- Toxicity Prevention: It helps to counteract negative charges that could negatively affect fungal growth.
- Permeability Regulation: Plays a role in regulating permeability within the fungi cell wall
- Metabolism regulation: Contributes to carbohydrate metabolism.
Effect of Temperature on Fungal Growth (Page 4)
- Procedure: Prepare Czapek Dox agar media, sterilize, pour into plates, allow to solidify, inoculate with tested fungi, and incubate at different temperatures (5°C, 15°C, 30°C, 45°C) for 7 days.
- Observation: Measure the diameter of fungal growth, record data.
- Minimum Temperature: The lowest temperature at which fungal growth is observed.
Optimum and Maximum Temperatures for Fungal Growth (Page 5)
- Optimum Temperature: The best temperature for fungal growth.
- Maximum Temperature: The highest temperature at which fungal growth is observed before it stalls.
- Fungal Types and Temperatures: Different fungal species (e.g., psychrophilic, mesophilic, thermophilic) are adapted to different temperature ranges.
- Low Temperature Impact: Low temperatures reduce the velocity (kinetics) of enzymes, limiting metabolic activity. Ice crystals formation in lower temperatures also affect fungal growth.
- High Temperature Impact: High temperatures denature enzymes, inhibiting growth. There is no absorption of nutrients.
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Description
This quiz explores the essential nutrient components needed for optimal fungal growth using Czapek Dox Media. You will learn about the preparation, sterilization, inoculation, and measurement processes involved in cultivating fungi. Discover how different nutrient limitations can affect fungal growth through this practical examination.
