10 Questions
What is the principle behind Southern blot technique for detecting food microorganisms?
Nucleic acid hybridization
What is the central concept involved in all processes of nucleic acids?
Base pairing
What are three molecular methods for detecting food microorganisms?
Culture-based, Nucleic acid-based, Antibody-based
What is the direction of a DNA strand, and why are the 5' and 3' ends not the same?
5' to 3'; due to sugar-phosphate linkages
How does hybridization work in the context of detecting food microorganisms?
Denature probe DNA, introduce unknown target DNA, hybridization occurs via base pairing
Describe the steps in Southern blotting.
The steps in Southern blotting include transferring DNA from a gel matrix to a filter, fixing the DNA to the filter, hybridizing with a labeled DNA probe, and washing unbound probe to determine the specific DNA sequence.
What is the basic principle of PCR?
The basic principle of PCR is to specifically target and amplify a single sequence from within a complex mixture of DNA.
What are the other types of blotting and the molecule that it detects?
Other types of blotting include Northern blotting (detects RNA) and Western blotting (detects proteins).
The DNA transferred/blotted onto the nitrocellulose membrane is finally dsDNA or ssDNA. Explain your answer.
The DNA transferred/blotted onto the nitrocellulose membrane is initially ssDNA, but after hybridization with a labeled DNA probe, it forms a dsDNA-hybridized complex.
Describe two methods in transferring DNA from agarose gel onto membrane.
Two methods for transferring DNA from agarose gel onto a membrane are capillary transfer and transfer using a gel reservoir.
Test your knowledge about the recent techniques for detecting and identifying food microorganisms, including Southern blot, FISH, PCR, and Real-Time PCR. Learn about the principles and applications of each method in detecting food microorganisms using molecular detection methods, culture-based techniques, nucleic acid-based methods, and antibody-based and aptamer-based approaches.
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