Food Microorganisms Molecular Detection Techniques Quiz

Nucleic acid hybridization

Base pairing

Culture-based, Nucleic acid-based, Antibody-based

<p>5' to 3'; due to sugar-phosphate linkages</p> Signup and view all the answers

<p>Denature probe DNA, introduce unknown target DNA, hybridization occurs via base pairing</p> Signup and view all the answers

<p>The steps in Southern blotting include transferring DNA from a gel matrix to a filter, fixing the DNA to the filter, hybridizing with a labeled DNA probe, and washing unbound probe to determine the specific DNA sequence.</p> Signup and view all the answers

<p>The basic principle of PCR is to specifically target and amplify a single sequence from within a complex mixture of DNA.</p> Signup and view all the answers

<p>Other types of blotting include Northern blotting (detects RNA) and Western blotting (detects proteins).</p> Signup and view all the answers

<p>The DNA transferred/blotted onto the nitrocellulose membrane is initially ssDNA, but after hybridization with a labeled DNA probe, it forms a dsDNA-hybridized complex.</p> Signup and view all the answers

<p>Two methods for transferring DNA from agarose gel onto a membrane are capillary transfer and transfer using a gel reservoir.</p> Signup and view all the answers