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Questions and Answers
What is the purpose of using a semipermeable membrane in protein separation?
What is the purpose of using a semipermeable membrane in protein separation?
- To concentrate the protein sample
- To retain molecules larger than the membrane cutoff (correct)
- To denature the proteins
- To allow smaller molecules to pass through
Which method of protein separation uses polyethylene glycol to concentrate the sample?
Which method of protein separation uses polyethylene glycol to concentrate the sample?
- Solvent Fractionation
- Iso-electric Precipitation
- Fractional Precipitation (correct)
- Size-Exclusion Chromatography
In membrane processes, what happens to molecules larger than the membrane cutoff?
In membrane processes, what happens to molecules larger than the membrane cutoff?
- They are denatured
- They become part of the filtrate
- They pass through the membrane
- They are retained and become part of the retentate (correct)
What is the main principle behind Size-Exclusion Chromatography?
What is the main principle behind Size-Exclusion Chromatography?
Which protein separation method involves separating proteins based on their isoelectric points?
Which protein separation method involves separating proteins based on their isoelectric points?
What is the principle behind Hydrophobic Interaction Chromatography?
What is the principle behind Hydrophobic Interaction Chromatography?
In Affinity Chromatography, what type of molecules can act as ligands for separating glycoproteins?
In Affinity Chromatography, what type of molecules can act as ligands for separating glycoproteins?
What is essential to consider when eluting proteins in Liquid Chromatography?
What is essential to consider when eluting proteins in Liquid Chromatography?
What is the purpose of eluting buffer in Protein Separation techniques?
What is the purpose of eluting buffer in Protein Separation techniques?
When would Hydrophobic Interaction Chromatography typically be employed?
When would Hydrophobic Interaction Chromatography typically be employed?
Why is the pI (isoelectric point) of a protein important in Protein Separation processes?
Why is the pI (isoelectric point) of a protein important in Protein Separation processes?
What is the main principle behind fractional precipitation in protein separation?
What is the main principle behind fractional precipitation in protein separation?
How does isoelectric precipitation work in protein separation?
How does isoelectric precipitation work in protein separation?
What is the purpose of solvent fractionation in protein separation?
What is the purpose of solvent fractionation in protein separation?
What effect does denaturation have on proteins in the context of protein separation?
What effect does denaturation have on proteins in the context of protein separation?
Which technique is NOT used for protein separation among the following options?
Which technique is NOT used for protein separation among the following options?
What is the primary factor involved in the salting out method of protein separation?
What is the primary factor involved in the salting out method of protein separation?
In solvent fractionation, why does the addition of acetone decrease protein solubility?
In solvent fractionation, why does the addition of acetone decrease protein solubility?
Which type of chromatography involves interactions based on charge differences?
Which type of chromatography involves interactions based on charge differences?
What happens to proteins during isoelectric focusing in electrophoresis?
What happens to proteins during isoelectric focusing in electrophoresis?
In protein separation techniques, which factor plays a significant role in determining a protein's interaction with other molecules?
In protein separation techniques, which factor plays a significant role in determining a protein's interaction with other molecules?
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