Food Analysis: Protein Separation and Characterization

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What is the purpose of using a semipermeable membrane in protein separation?

To concentrate the protein sample

To retain molecules larger than the membrane cutoff (correct)

To denature the proteins

To allow smaller molecules to pass through

Which method of protein separation uses polyethylene glycol to concentrate the sample?

Solvent Fractionation

Iso-electric Precipitation

Fractional Precipitation (correct)

Size-Exclusion Chromatography

In membrane processes, what happens to molecules larger than the membrane cutoff?

They are denatured

They become part of the filtrate

They pass through the membrane

They are retained and become part of the retentate (correct)

What is the main principle behind Size-Exclusion Chromatography?

Separating proteins based on size Signup and view all the answers

Which protein separation method involves separating proteins based on their isoelectric points?

Iso-electric Precipitation Signup and view all the answers

What is the principle behind Hydrophobic Interaction Chromatography?

Increased hydrophobic interactions at high ionic strength Signup and view all the answers

In Affinity Chromatography, what type of molecules can act as ligands for separating glycoproteins?

Antibodies Signup and view all the answers

What is essential to consider when eluting proteins in Liquid Chromatography?

pI of the proteins Signup and view all the answers

What is the purpose of eluting buffer in Protein Separation techniques?

To gradually change ionic strength or pH Signup and view all the answers

When would Hydrophobic Interaction Chromatography typically be employed?

Following ammonium sulfate precipitation of proteins Signup and view all the answers

Why is the pI (isoelectric point) of a protein important in Protein Separation processes?

It influences the pH at which the protein will have no net charge Signup and view all the answers

What is the main principle behind fractional precipitation in protein separation?

Proteins are precipitated based on their solubility Signup and view all the answers

How does isoelectric precipitation work in protein separation?

Proteins are precipitated at their isoelectric point due to charge attraction Signup and view all the answers

What is the purpose of solvent fractionation in protein separation?

To decrease protein solubility Signup and view all the answers

What effect does denaturation have on proteins in the context of protein separation?

It leads to protein precipitation from the solution Signup and view all the answers

Which technique is NOT used for protein separation among the following options?

Polymerase Chain Reaction (PCR) Signup and view all the answers

What is the primary factor involved in the salting out method of protein separation?

Ionic Strength Signup and view all the answers

In solvent fractionation, why does the addition of acetone decrease protein solubility?

By decreasing the dielectric constant of the solution Signup and view all the answers

Which type of chromatography involves interactions based on charge differences?

Ion-Exchange Chromatography Signup and view all the answers

What happens to proteins during isoelectric focusing in electrophoresis?

They migrate based on their charge Signup and view all the answers

In protein separation techniques, which factor plays a significant role in determining a protein's interaction with other molecules?

Biological Affinities Signup and view all the answers