21 Questions
What is the purpose of using a semipermeable membrane in protein separation?
To retain molecules larger than the membrane cutoff
Which method of protein separation uses polyethylene glycol to concentrate the sample?
Fractional Precipitation
In membrane processes, what happens to molecules larger than the membrane cutoff?
They are retained and become part of the retentate
What is the main principle behind Size-Exclusion Chromatography?
Separating proteins based on size
Which protein separation method involves separating proteins based on their isoelectric points?
Iso-electric Precipitation
What is the principle behind Hydrophobic Interaction Chromatography?
Increased hydrophobic interactions at high ionic strength
In Affinity Chromatography, what type of molecules can act as ligands for separating glycoproteins?
Antibodies
What is essential to consider when eluting proteins in Liquid Chromatography?
pI of the proteins
What is the purpose of eluting buffer in Protein Separation techniques?
To gradually change ionic strength or pH
When would Hydrophobic Interaction Chromatography typically be employed?
Following ammonium sulfate precipitation of proteins
Why is the pI (isoelectric point) of a protein important in Protein Separation processes?
It influences the pH at which the protein will have no net charge
What is the main principle behind fractional precipitation in protein separation?
Proteins are precipitated based on their solubility
How does isoelectric precipitation work in protein separation?
Proteins are precipitated at their isoelectric point due to charge attraction
What is the purpose of solvent fractionation in protein separation?
To decrease protein solubility
What effect does denaturation have on proteins in the context of protein separation?
It leads to protein precipitation from the solution
Which technique is NOT used for protein separation among the following options?
Polymerase Chain Reaction (PCR)
What is the primary factor involved in the salting out method of protein separation?
Ionic Strength
In solvent fractionation, why does the addition of acetone decrease protein solubility?
By decreasing the dielectric constant of the solution
Which type of chromatography involves interactions based on charge differences?
Ion-Exchange Chromatography
What happens to proteins during isoelectric focusing in electrophoresis?
They migrate based on their charge
In protein separation techniques, which factor plays a significant role in determining a protein's interaction with other molecules?
Biological Affinities
This quiz covers methods of protein separation including fractional precipitation, liquid chromatography, size separation, and electrophoresis. It also includes protein characterization procedures such as amino acid analysis, assessment of protein nutritional quality, and functional properties evaluation.
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