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Fluorescence Recovery After Photobleaching (FRAP)
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Fluorescence Recovery After Photobleaching (FRAP)

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Questions and Answers

Which technique is used to quantify the lateral movements of specific plasma-membrane proteins and lipids?

  • Fluorescence recovery after photobleaching (FRAP) (correct)
  • Diffusion coefficient determination
  • Fluorescent probe labeling
  • Laser beam irradiation
  • What is the purpose of labeling the lipids in the outer leaflet of the plasma membrane with a membrane-impermeant fluorescent probe?

  • To make the lipids non-fluorescent
  • To measure the diffusion coefficient of the labeled lipid
  • To quantify the lateral movements of membrane proteins (correct)
  • To separate protein-rich regions from lipid-rich regions
  • What happens to a small area of the plasma membrane when it is bleached by irradiation with an intense laser beam?

  • It becomes fluorescent
  • It becomes non-fluorescent (correct)
  • It separates protein-rich regions from lipid-rich regions
  • It diffuses into the bleached region
  • What does the time course of fluorescence return to the bleached region determine?

    <p>The diffusion coefficient of the labeled lipid</p> Signup and view all the answers

    What does the FRAP method measure?

    <p>The diffusion coefficient of the labeled lipid</p> Signup and view all the answers

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