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Questions and Answers
Which technique is used to quantify the lateral movements of specific plasma-membrane proteins and lipids?
Which technique is used to quantify the lateral movements of specific plasma-membrane proteins and lipids?
- Fluorescence recovery after photobleaching (FRAP) (correct)
- Diffusion coefficient determination
- Fluorescent probe labeling
- Laser beam irradiation
What is the purpose of labeling the lipids in the outer leaflet of the plasma membrane with a membrane-impermeant fluorescent probe?
What is the purpose of labeling the lipids in the outer leaflet of the plasma membrane with a membrane-impermeant fluorescent probe?
- To make the lipids non-fluorescent
- To measure the diffusion coefficient of the labeled lipid
- To quantify the lateral movements of membrane proteins (correct)
- To separate protein-rich regions from lipid-rich regions
What happens to a small area of the plasma membrane when it is bleached by irradiation with an intense laser beam?
What happens to a small area of the plasma membrane when it is bleached by irradiation with an intense laser beam?
- It becomes fluorescent
- It becomes non-fluorescent (correct)
- It separates protein-rich regions from lipid-rich regions
- It diffuses into the bleached region
What does the time course of fluorescence return to the bleached region determine?
What does the time course of fluorescence return to the bleached region determine?
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What does the FRAP method measure?
What does the FRAP method measure?
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Study Notes
Fluorescence Recovery After Photobleaching (FRAP)
- FRAP is a technique used to quantify the lateral movements of specific plasma-membrane proteins and lipids.
- Labeling the lipids in the outer leaflet of the plasma membrane with a membrane-impermeant fluorescent probe allows researchers to track their movement within the cell membrane.
- When a small area of the plasma membrane is bleached by irradiation with an intense laser beam, the fluorescence in that area is extinguished.
- The time course of fluorescence return to the bleached region determines the rate of diffusion of the labeled molecules.
- FRAP measures the diffusion coefficient of the labeled molecules, which reflects the fluidity of the cell membrane and the mobility of the labeled proteins and lipids.
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