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Questions and Answers
What distinguishes actinolite fibres from glass fibres?
What distinguishes actinolite fibres from glass fibres?
Which type of microscopy involves capturing images from different focal planes that are then recombined into a 3D image?
Which type of microscopy involves capturing images from different focal planes that are then recombined into a 3D image?
What is the main difference between widefield and confocal microscopy?
What is the main difference between widefield and confocal microscopy?
Which type of fluorescence microscopy uses a single antibody conjugated to a fluorophore?
Which type of fluorescence microscopy uses a single antibody conjugated to a fluorophore?
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How are images captured in confocal microscopy?
How are images captured in confocal microscopy?
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Which type of microscopy involves Stimulated Emission Depletion (STED) microscopy, Structured Illumination Microscopy (SIM), and Stochastic Optical Reconstruction Microscopy (STORM)/Photo-activation Localization Microscopy (PALM)?
Which type of microscopy involves Stimulated Emission Depletion (STED) microscopy, Structured Illumination Microscopy (SIM), and Stochastic Optical Reconstruction Microscopy (STORM)/Photo-activation Localization Microscopy (PALM)?
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Which type of microscopy is best suited for viewing stained or naturally pigmented specimens?
Which type of microscopy is best suited for viewing stained or naturally pigmented specimens?
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In which type of microscopy is it best to view everything in a liquid sample, including debris?
In which type of microscopy is it best to view everything in a liquid sample, including debris?
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Which microscopy technique is particularly useful for finding cells in suspension?
Which microscopy technique is particularly useful for finding cells in suspension?
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Which microscopy technique makes it easy to obtain the correct focal plane at low magnification for small, low contrast specimens?
Which microscopy technique makes it easy to obtain the correct focal plane at low magnification for small, low contrast specimens?
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In which type of microscopy is specialized staining and immunohistochemistry (IHC) detailed later in the course?
In which type of microscopy is specialized staining and immunohistochemistry (IHC) detailed later in the course?
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Which type of microscopy is best suited for viewing unstained living cells?
Which type of microscopy is best suited for viewing unstained living cells?
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What type of microscopy reveals orientational order in native molecular structures inside living cells, tissues, and whole organisms?
What type of microscopy reveals orientational order in native molecular structures inside living cells, tissues, and whole organisms?
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Which microscopy technique uses a high-intensity light source to excite a fluorescent molecule called a fluorophore?
Which microscopy technique uses a high-intensity light source to excite a fluorescent molecule called a fluorophore?
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What type of microscopy is used extensively in optical mineralogy and forensic science for identifying trace evidence found at crime scenes?
What type of microscopy is used extensively in optical mineralogy and forensic science for identifying trace evidence found at crime scenes?
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Which microscopy technique is particularly advantageous when observing living specimens due to its ability to visualize unstained specimens?
Which microscopy technique is particularly advantageous when observing living specimens due to its ability to visualize unstained specimens?
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What type of microscopy combines the magnifying properties of the light microscope with the fluorescence emitting properties of compounds?
What type of microscopy combines the magnifying properties of the light microscope with the fluorescence emitting properties of compounds?
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Which of the following microscopies is not ideal for visualizing transparent specimens when high-resolution is not required?
Which of the following microscopies is not ideal for visualizing transparent specimens when high-resolution is not required?
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Study Notes
Fluorescence Microscopy
- Actinolite fibers exhibit prominent colors due to birefringence, which distinguishes them from glass fibers that lack birefringence.
- Types of fluorescence include native fluorescence (autofluorescence) and primary immunofluorescence, which uses a single antibody conjugated to a fluorophore.
- Secondary fluorescence employs two antibodies: an unlabeled primary antibody that binds to the target molecule and a secondary antibody carrying the fluorophore.
Confocal Microscopy
- Widefield microscopy versus confocal microscopy: confocal microscopy captures images from different focal planes and recombines them into a 3D image.
Super-Resolution Microscopy (SRM)
- SRM types include stimulated emission depletion (STED) microscopy, structured illumination microscopy (SIM), and stochastic optical reconstruction microscopy (STORM)/photo-activation localization microscopy (PALM).
Phase Contrast and Differential Interference Contrast (DIC)
- Phase contrast is used to visualize transparent specimens when high-resolution is not required, such as living cells, microorganisms, and tissues.
- DIC is useful for visualizing unstained specimens, like living cells, and allows for observing living specimens without staining.
Polarized Microscopy
- Polarized light microscopy reveals orientational order in native molecular structures inside living cells, tissues, and whole organisms.
- It is commonly used in forensic science to identify and characterize trace evidence at crime scenes.
Advanced Optical and Imaging Platform
- Applications of optical microscopy include bright field, dark field, phase contrast, and fluorescence microscopy.
Bright Field and Dark Field Microscopy
- Bright field microscopy is suited for viewing stained or naturally pigmented specimens.
- Dark field microscopy is best for viewing samples in a liquid, including debris, and is useful for finding cells in suspension.
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Description
Test your knowledge on fluorescence microscopy and fibre identification, including distinguishing actinolite fibres from glass fibres based on birefringence. Explore different types of fluorescence techniques such as native fluorescence, primary immunofluorescence, and secondary fluorescence.
