18 Questions
What distinguishes actinolite fibres from glass fibres?
Birefringence
Which type of microscopy involves capturing images from different focal planes that are then recombined into a 3D image?
Confocal Microscopy
What is the main difference between widefield and confocal microscopy?
Image capturing technique
Which type of fluorescence microscopy uses a single antibody conjugated to a fluorophore?
Primary immunofluorescence
How are images captured in confocal microscopy?
By moving the stage in direction Z
Which type of microscopy involves Stimulated Emission Depletion (STED) microscopy, Structured Illumination Microscopy (SIM), and Stochastic Optical Reconstruction Microscopy (STORM)/Photo-activation Localization Microscopy (PALM)?
Super-resolution Microscopy
Which type of microscopy is best suited for viewing stained or naturally pigmented specimens?
Bright Field Microscopy
In which type of microscopy is it best to view everything in a liquid sample, including debris?
Dark Field Microscopy
Which microscopy technique is particularly useful for finding cells in suspension?
Dark Field Microscopy
Which microscopy technique makes it easy to obtain the correct focal plane at low magnification for small, low contrast specimens?
Confocal Microscopy
In which type of microscopy is specialized staining and immunohistochemistry (IHC) detailed later in the course?
Bright Field Microscopy
Which type of microscopy is best suited for viewing unstained living cells?
Confocal Microscopy
What type of microscopy reveals orientational order in native molecular structures inside living cells, tissues, and whole organisms?
Polarized Light Microscopy
Which microscopy technique uses a high-intensity light source to excite a fluorescent molecule called a fluorophore?
Fluorescence Microscopy
What type of microscopy is used extensively in optical mineralogy and forensic science for identifying trace evidence found at crime scenes?
Polarized Light Microscopy
Which microscopy technique is particularly advantageous when observing living specimens due to its ability to visualize unstained specimens?
Differential Interference Contrast
What type of microscopy combines the magnifying properties of the light microscope with the fluorescence emitting properties of compounds?
Fluorescence Microscopy
Which of the following microscopies is not ideal for visualizing transparent specimens when high-resolution is not required?
Confocal Microscopy
Study Notes
Fluorescence Microscopy
- Actinolite fibers exhibit prominent colors due to birefringence, which distinguishes them from glass fibers that lack birefringence.
- Types of fluorescence include native fluorescence (autofluorescence) and primary immunofluorescence, which uses a single antibody conjugated to a fluorophore.
- Secondary fluorescence employs two antibodies: an unlabeled primary antibody that binds to the target molecule and a secondary antibody carrying the fluorophore.
Confocal Microscopy
- Widefield microscopy versus confocal microscopy: confocal microscopy captures images from different focal planes and recombines them into a 3D image.
Super-Resolution Microscopy (SRM)
- SRM types include stimulated emission depletion (STED) microscopy, structured illumination microscopy (SIM), and stochastic optical reconstruction microscopy (STORM)/photo-activation localization microscopy (PALM).
Phase Contrast and Differential Interference Contrast (DIC)
- Phase contrast is used to visualize transparent specimens when high-resolution is not required, such as living cells, microorganisms, and tissues.
- DIC is useful for visualizing unstained specimens, like living cells, and allows for observing living specimens without staining.
Polarized Microscopy
- Polarized light microscopy reveals orientational order in native molecular structures inside living cells, tissues, and whole organisms.
- It is commonly used in forensic science to identify and characterize trace evidence at crime scenes.
Advanced Optical and Imaging Platform
- Applications of optical microscopy include bright field, dark field, phase contrast, and fluorescence microscopy.
Bright Field and Dark Field Microscopy
- Bright field microscopy is suited for viewing stained or naturally pigmented specimens.
- Dark field microscopy is best for viewing samples in a liquid, including debris, and is useful for finding cells in suspension.
Test your knowledge on fluorescence microscopy and fibre identification, including distinguishing actinolite fibres from glass fibres based on birefringence. Explore different types of fluorescence techniques such as native fluorescence, primary immunofluorescence, and secondary fluorescence.
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