Fluorescence and Confocal Microscopy Outline

Photoreactive chemicals

It is emitted at a lower energy level as fluorescence

To allow detection of antigen-antibody complexes

<p>Frozen, non-fixed tissues and ethanol fixed tissues</p> Signup and view all the answers

<p>To identify patterns of protein expression in cells</p> Signup and view all the answers

<p>To absorb light and emit it with lower energy</p> Signup and view all the answers

<p>To minimize non-specific staining</p> Signup and view all the answers

<p>FITC</p> Signup and view all the answers

<p>PBS/Glycerol mix</p> Signup and view all the answers

<p>Photobleaching</p> Signup and view all the answers

<p>Trans-pyridine-2-azo-p-dimethylaniline (PADA)</p> Signup and view all the answers

<p>To permeabilize the cells</p> Signup and view all the answers

<p>Sheep anti-p53 polyconal</p> Signup and view all the answers

<p>To locate and identify patterns of protein expression in cells.</p> Signup and view all the answers

<p>Fluorochromes absorb light in a specific wavelength range and re-emit it with lower energy, allowing the visualization of fluorescent samples.</p> Signup and view all the answers

<p>Frozen, non-fixed tissues and ethanol fixed tissues.</p> Signup and view all the answers

<p>Fluorochromes re-emit the absorbed light at a longer wavelength with lower energy, a process known as fluorescence.</p> Signup and view all the answers

<p>Confocal microscopy provides higher resolution and better 3-dimensional imaging compared to conventional fluorescent microscopy.</p> Signup and view all the answers

<p>To bind to the antibody-antigen complex and allow detection of the complex through fluorescence.</p> Signup and view all the answers

<p>trans-pyridine-2-azo-p-dimethylaniline (PADA)</p> Signup and view all the answers

<p>FITC</p> Signup and view all the answers

<p>To bind to the primary antibody and aid in visualization under a microscope.</p> Signup and view all the answers

<p>Fluorochromes absorb light at one wavelength and emit it at a longer wavelength, producing fluorescence, which aids in visualization under the microscope.</p> Signup and view all the answers

<p>Paraformaldehyde or methanol/acetone</p> Signup and view all the answers

<p>The tendency of fluorochromes to lose fluorescence upon excitation by a high energy light source, known as photobleaching.</p> Signup and view all the answers

<p>To permeabilize the cells, allowing antibodies to enter and bind to intracellular structures or proteins.</p> Signup and view all the answers

<p>Mounting medium, usually a PBS/Glycerol mix</p> Signup and view all the answers

<p>Sheep anti-p53 polyconal</p> Signup and view all the answers

<p>Texas Red</p> Signup and view all the answers