10 Questions
What is the definition of quantum yield in the context of fluorescence?
The ratio of the number of emitted to absorbed photons
Which environmental factor is known to affect the quantum yield of a fluorescent probe?
Fluorochrome concentration
What is the definition of fluorescence lifetime?
The average time the electron spends in the excited state before returning to the ground state
What is the definition of fluorescence quenching?
The phenomenon by which interaction of a molecule with the fluorochrome reduces the quantum yield or the lifetime
What is the definition of autofluorescence?
Fluorescence that does not originate from the fluorochrome of interest
What is the definition of photobleaching?
A gradual loss of fluorescence intensity of the specimen arising from interaction between the exciting light and the fluorescent compound
What is the primary function of a CCD camera in fluorescence microscopy?
To detect and amplify the fluorescence signal
What is the primary function of a photomultiplier tube (PMT) in fluorescence microscopy?
To detect and amplify the fluorescence signal
Which of the following is a key parameter for quantifying fluorescence intensity in microscopy?
Quantum yield
How does photobleaching affect the collection of high-quality image data in fluorescence microscopy?
Photobleaching interferes with the collection of high-quality image data by causing a gradual loss of fluorescence intensity
Explore the measurement of fluorescence intensity and concentration of labeled targets in fluorescence microscopy. Learn about estimating local and absolute concentrations of fluorescent molecules based on image analysis.
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