Fluorescence Intensity and Labeled Target Concentration Measurements

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Questions and Answers

What is the definition of quantum yield in the context of fluorescence?

The phenomenon by which interaction of a molecule with the fluorochrome reduces the quantum yield or the lifetime
The average time the electron spends in the excited state before returning to the ground state
Fluorescence that does not originate from the fluorochrome of interest
The ratio of the number of emitted to absorbed photons (correct)

Which environmental factor is known to affect the quantum yield of a fluorescent probe?

Photobleaching
Fluorescence quenching
Fluorescence lifetime
Fluorochrome concentration (correct)

What is the definition of fluorescence lifetime?

Fluorescence that does not originate from the fluorochrome of interest
The average time the electron spends in the excited state before returning to the ground state (correct)
The ratio of the number of emitted to absorbed photons
The phenomenon by which interaction of a molecule with the fluorochrome reduces the quantum yield or the lifetime

What is the definition of fluorescence quenching?

The phenomenon by which interaction of a molecule with the fluorochrome reduces the quantum yield or the lifetime (B) Signup and view all the answers

What is the definition of autofluorescence?

Fluorescence that does not originate from the fluorochrome of interest (A) Signup and view all the answers

What is the definition of photobleaching?

A gradual loss of fluorescence intensity of the specimen arising from interaction between the exciting light and the fluorescent compound (D) Signup and view all the answers

What is the primary function of a CCD camera in fluorescence microscopy?

To detect and amplify the fluorescence signal (C) Signup and view all the answers

What is the primary function of a photomultiplier tube (PMT) in fluorescence microscopy?

To detect and amplify the fluorescence signal (A) Signup and view all the answers

Which of the following is a key parameter for quantifying fluorescence intensity in microscopy?

Quantum yield (A) Signup and view all the answers

How does photobleaching affect the collection of high-quality image data in fluorescence microscopy?

Photobleaching interferes with the collection of high-quality image data by causing a gradual loss of fluorescence intensity (A) Signup and view all the answers