18 Questions
What is the primary reason for not shaking or stirring the melted medium?
To prevent the formation of air bubbles
What is a characteristic of Farrant's medium that sets it apart from other mediums?
It does not solidify upon storage
What is the purpose of adding potassium acetate to Farrant's medium?
To raise the pH of the medium
What is the primary use of Apathy's medium?
For mounting nerve preparations
What is the pH of Apathy's medium?
Near 4.0
What is the substitute for sodium merthiolate in Farrant's medium?
Arsenic trioxide
Which of the following mounting media is generally suitable for all enzymatic label/chromogen combinations and fluorescent labels?
Aqueous mounting medium
What is the effect of glycerol on phycobiliprotein fluorescent intensity labels?
Quenches the staining
What is the result of exposing most fluorescent labels to excitation light?
Diminished staining
What is the alternative to glycerine jelly for fluorescent labels?
Polyvinyl alcohol
What is the purpose of adding paraphenylene-diamine to Polyvinyl alcohol?
As an antifading agent
What is the method to recover a broken slide?
All of the above
What is the purpose of adding potassium acetate, calcium chloride, or sodium chloride to a medium?
To raise the pH to near 7.0 and prevent 'bleeding' of metachromatic stains for amyloid
What type of resin is used for embedding undecalcified bones and for electron microscopy?
Synthetic resin
What is the main advantage of using Brun's fluid for mounting frozen sections?
It sets quite hard and does not require ringing
What is the recommended mounting medium for frozen sections from water?
Brun's fluid
What is the refractive index of Canada balsam?
1.524
How is Canada balsam usually dissolved?
In xylene in an incubator at 37°C
Learn how to prepare Farrant's Medium, a mixture used in laboratory settings. Understand the ingredients, ratios, and steps involved in creating this medium, and why it's important to avoid air bubbles.
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