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Questions and Answers
What is the primary purpose of wearing protective gear in a laboratory?
What is the primary purpose of wearing protective gear in a laboratory?
- To impress instructors with laboratory attire
- To prevent exposure to potential hazards (correct)
- To enhance visibility of microscopic samples
- To follow laboratory fashion trends
What is the function of the objective lens in a microscope?
What is the function of the objective lens in a microscope?
- Focuses light onto the specimen (correct)
- Holds the slide in place
- Adjusts the microscope's zoom
- Magnifies the image
What is the purpose of staining in microbiology?
What is the purpose of staining in microbiology?
- To enhance contrast and visibility (correct)
- To identify specific microorganisms
- To prepare the specimen for incubation
- To sterilize the specimen
What is the primary purpose of sterilization in microbiology?
What is the primary purpose of sterilization in microbiology?
What is the purpose of cutting in dissection techniques?
What is the purpose of cutting in dissection techniques?
What is a key aspect of fieldwork in ecology and environmental science?
What is a key aspect of fieldwork in ecology and environmental science?
Flashcards
Purpose of protective lab gear?
Purpose of protective lab gear?
To prevent contact with chemical, biological, or physical risks present in the lab.
Objective lens function?
Objective lens function?
To magnify the image of the specimen.
Why stain in microbiology?
Why stain in microbiology?
Increase contrast, enabling better visualization of microorganisms.
Sterilization purpose?
Sterilization purpose?
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Cutting in dissection?
Cutting in dissection?
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Fieldwork key aspect?
Fieldwork key aspect?
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Study Notes
Laboratory Safety
- Wear protective gear: lab coats, gloves, goggles, and closed-toe shoes
- Handle equipment and chemicals with care
- Follow instructions and protocols carefully
- Dispose of waste and materials properly
- Report accidents and incidents to the instructor immediately
Microscopy
- Types of microscopes:
- Light microscope: uses visible light
- Electron microscope: uses electron beam
- Parts of a microscope:
- Eyepiece: magnifies image
- Objective lens: focuses light
- Stage: holds slide
- Preparing slides:
- Fixing: preserves specimen
- Staining: enhances contrast
- Mounting: attaches specimen to slide
Microbiology
- Aseptic techniques:
- Sterilization: kills all microorganisms
- Disinfection: reduces microorganisms
- Microbial growth:
- Culture media: supports growth
- Incubation: provides optimal conditions
- Identification methods:
- Staining: Gram, acid-fast, etc.
- Biochemical tests: e.g., API, VITEK
Dissection
- Preparation:
- Fixing: preserves specimen
- Disinfection: reduces microorganisms
- Dissection techniques:
- Cutting: uses scissors and scalpels
- Probing: uses forceps and probes
- Identification of structures:
- Observation: uses hand lens and microscope
- Measurement: uses calipers and rulers
Ecology and Environmental Science
- Fieldwork:
- Sampling: collects data and specimens
- Observation: records environmental conditions
- Laboratory analysis:
- Water quality: pH, turbidity, etc.
- Soil analysis: pH, texture, etc.
- Data analysis:
- Graphs: visualizes data
- Statistical tests: e.g., t-test, ANOVA
Genetics and Molecular Biology
- DNA extraction:
- Isolation: separates DNA from cells
- Purification: removes impurities
- PCR (Polymerase Chain Reaction):
- Amplification: increases DNA copy number
- Primers: specific DNA sequences
- Gel electrophoresis:
- Separation: based on size and charge
- Visualization: uses dyes and UV light
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