30 Questions
Which of the following best defines enzyme kinetics?
The study of chemical reactions that are catalyzed by enzymes
What does the Michaelis-Menten equation describe?
How enzyme kinetics vary with substrate concentration
Who were the scientists behind the Michaelis-Menten equation?
Leonor Michaelis and Maud Menten
What is the Lineweaver-Burk plot used for?
To interpret 1/Vmax and 1/Km
What are the two types of enzyme inhibition?
Competitive and noncompetitive
How can competitive and noncompetitive inhibitors be explained using the Michaelis-Menten curve and Lineweaver-Burk plot?
By interpreting 1/Vmax and 1/Km values
What is an example of an enzyme inhibitor?
Drugs
Which factor affects the rate of an enzyme-catalyzed reaction until a maximal velocity is reached?
Substrate concentration
What happens to the reaction velocity when the temperature is further elevated beyond the optimal temperature for most mammalian enzymes?
It decreases
How does extreme pH conditions affect the reaction velocity of enzymes?
It decreases the reaction velocity
What is the effect of a competitive inhibitor on the Vmax of an enzyme-catalyzed reaction?
It has no effect on Vmax
How does a competitive inhibitor affect the Km for a given substrate?
It increases Km
What is the effect of a competitive inhibitor on the Lineweaver-Burk plot?
Vmax is unchanged
What is the effect of irreversible inhibitors on enzymes?
They denature enzymes
Which of the following is true about the Michaelis-Menten kinetics?
Km is equal to the substrate concentration at which the reaction velocity is ½ Vmax
Which of the following conclusions can be drawn from the Michaelis-Menten equation?
Enzyme 1 has a high affinity for its substrate because a low concentration of substrate is needed to half-saturate the enzyme
Which of the following statements is true about the oxyhemoglobin dissociation curve?
It shows a sigmoidal curve
Which type of kinetics do allosteric enzymes exhibit?
Sigmoidal curve
Which of the following is true about the Lineweaver-Burk plot?
It is used to calculate Km and Vmax
Which order of reaction is observed when [S] << Km?
Zero order
Which of the following is true about the total enzyme concentration [E] in the Michaelis-Menten kinetics?
It does not change over time
Which enzyme do statin drugs inhibit?
Cholesterol biosynthesis hydroxymethylglutaryl-CoA reductase
What is the mechanism of action of statin drugs?
Prevent de novo cholesterol synthesis
Where do noncompetitive inhibitors bind?
Allosteric site
What is the effect of noncompetitive inhibitors on Vmax?
Decrease Vmax
What is the effect of noncompetitive inhibitors on Km?
Km remains unchanged
Which type of inhibitors bind at a site different from the substrate?
Noncompetitive inhibitors
What is the main target of β-lactam antibiotics?
Enzymes important for bacterial cell wall synthesis
What is the mechanism of action of ACE inhibitors?
Block the enzyme that cleaves angiotensin I to angiotensin II
What does aspirin irreversibly inhibit?
Prostaglandin and thromboxane synthesis
Test your knowledge on the factors influencing reaction velocity in biochemistry, specifically focusing on enzymes and substrate concentration. Explore how enzymes function in living cells and their varying responses to changes in substrate concentration.
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