15 Questions
What is the purpose of adding (NH4)2SO4 during extraction?
To enhance the solubility of glycosides
What is the primary advantage of freeze-drying over other extraction methods?
It allows for the extraction of thermolabile compounds
What is the main disadvantage of boiling acetone as an extraction method?
It can cause the degradation of glycosides
Why is extraction at very low temperatures important in plant-insect interactions?
To understand the role of temperature in plant defense mechanisms
What is the primary concern when extracting glycosides using a method that involves heating?
The degradation of glycosides
What is the primary purpose of the purification steps mentioned?
To remove impurities from the extracted glycosides
What is the likely outcome if the purification steps are not followed?
Impurities in the extracted glycosides
What is the primary chemistry involved in the extraction and isolation of glycosides?
Glycoside chemistry
What is the potential application of the purified glycosides?
Pharmaceutical formulations
What is the likely source of the glycosides undergoing purification?
Plant extraction
What is the primary purpose of washing with ether in the extraction process?
To defat the plant material and improve extraction efficiency
What is the most likely reason for using 80% ethanol in the extraction process?
To solubilize and extract polar compounds from the plant material
What is the potential consequence of not defatting the plant material with ether?
Reduced extraction of lipophilic compounds and altered extract composition
What is the purpose of filtering the plant extract after extraction with 80% ethanol?
To remove impurities and sediment from the extract
What is the potential consequence of not concentrating the plant extract after extraction?
Increased difficulty in isolating target compounds from the extract
Study Notes
Extraction Methods
- Boiling acetone is a method used for extraction
- Extraction can be done at very low temperatures
- Freeze-drying is another method for extraction
- Extraction can be done in the presence of (NH4)2SO4
Purification of Extracted Glycosides
- Extract the plant with 80% ethanol to purify glycosides
- Concentrate the extract and filter it
- Wash with ether for defatting to remove fats
This quiz covers various methods of extraction, including boiling acetone, extraction at low temperatures, freeze-drying, and extraction in the presence of ammonium sulfate.
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