Enzymology STB3613: Methods for Studying Enzymes
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Questions and Answers

What is a common problem encountered when studying enzymes?

  • Enzymes do not undergo conformational changes
  • Large amount of enzymes are not required
  • All enzyme reactions are slow and easily measurable
  • Ligands and enzymes do not remain in the same bound and unbound states (correct)

    • How can the size difference between bound and unbound ligands be determined?

  • By measuring the pH difference
  • By conducting equilibrium dialysis
  • By observing color changes
  • By using gel chromatography (correct)

    • What is the advantage of equilibrium dialysis in studying enzymes?

  • It leads to rapid dissociation of bound ligands
  • It allows for direct measurement of bound ligand concentrations (correct)
  • It does not require a semi-permeable membrane
  • It provides an easy way to change buffer solutions

    • What can cause spectral shifts in the study of enzyme binding?

    <p>Conformational changes</p> Signup and view all the answers

    What is the purpose of size determination methods in studying enzyme binding?

    <p>To distinguish between bound and unbound ligands</p> Signup and view all the answers

    What is the purpose of ultrafiltration?

    <p>Separating macromolecules from low molecular weight components</p> Signup and view all the answers

    What is the primary purpose of gel filtration?

    <p>Purification, molecular mass determination, and desalting</p> Signup and view all the answers

    What is the distinguishing feature of ultracentrifugation?

    <p>Preparative manner for purification and concentration</p> Signup and view all the answers

    What can electrochemical methods be used for?

    <p>Reactions causing pH changes</p> Signup and view all the answers

    What are spectroscopic methods widely used for?

    <p>Kinetics studies, enzyme tests, ligand binding, conformation changes</p> Signup and view all the answers

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