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Questions and Answers
What is a common problem encountered when studying enzymes?
What is a common problem encountered when studying enzymes?
- Enzymes do not undergo conformational changes
- Large amount of enzymes are not required
- All enzyme reactions are slow and easily measurable
- Ligands and enzymes do not remain in the same bound and unbound states (correct)
How can the size difference between bound and unbound ligands be determined?
How can the size difference between bound and unbound ligands be determined?
- By measuring the pH difference
- By conducting equilibrium dialysis
- By observing color changes
- By using gel chromatography (correct)
What is the advantage of equilibrium dialysis in studying enzymes?
What is the advantage of equilibrium dialysis in studying enzymes?
- It leads to rapid dissociation of bound ligands
- It allows for direct measurement of bound ligand concentrations (correct)
- It does not require a semi-permeable membrane
- It provides an easy way to change buffer solutions
What can cause spectral shifts in the study of enzyme binding?
What can cause spectral shifts in the study of enzyme binding?
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What is the purpose of size determination methods in studying enzyme binding?
What is the purpose of size determination methods in studying enzyme binding?
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What is the purpose of ultrafiltration?
What is the purpose of ultrafiltration?
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What is the primary purpose of gel filtration?
What is the primary purpose of gel filtration?
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What is the distinguishing feature of ultracentrifugation?
What is the distinguishing feature of ultracentrifugation?
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What can electrochemical methods be used for?
What can electrochemical methods be used for?
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What are spectroscopic methods widely used for?
What are spectroscopic methods widely used for?
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