12 Questions
What is the name of the site-specific restriction nuclease discovered in 1968?
HindII
What is the source of T4 ligase used for DNA ligation?
Bacteriophage T4
How many types of restriction nucleases have been reported/studied?
2
Which of the following is a feature of the pBR322 plasmid?
Contains tetracycline resistance
Where would you select a gene for recombinant gene expression?
NCBI database
What is a distinguishing feature of expression plasmids in comparison to cloning plasmids?
Contain strictly controlled gene expression by using various promoters
Which type of vector generally has low copy number per cell?
pET vectors
What is the main function of restriction enzymes in recombinant DNA technology?
Recognizing specific sequences and cutting the DNA producing fragments
What was observed in the 1950s that led to the discovery of restriction enzymes?
No phage growth in certain bacterial strains
Which tag is available in pET plasmids for detection and purification of expressed proteins?
Histidine tag
Which plasmid has a ColE1 origin of replication?
pBR322 plasmid
What is the role of ligases in recombinant DNA technology?
Joining DNA fragments together
Test your knowledge on the tools used for engineering proteins in the production of recombinant proteins. This quiz covers topics such as cloning vectors, expression vectors, restriction enzymes, ligases, and DNA polymerases.
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