Podcast
Questions and Answers
What is the name of the site-specific restriction nuclease discovered in 1968?
What is the name of the site-specific restriction nuclease discovered in 1968?
- NotI
- BamHI
- EcoRI
- HindII (correct)
What is the source of T4 ligase used for DNA ligation?
What is the source of T4 ligase used for DNA ligation?
- Escherichia coli
- Bacillus subtilis
- Bacteriophage T4 (correct)
- Saccharomyces cerevisiae
How many types of restriction nucleases have been reported/studied?
How many types of restriction nucleases have been reported/studied?
- 10
- 5
- 2 (correct)
- 20
Which of the following is a feature of the pBR322 plasmid?
Which of the following is a feature of the pBR322 plasmid?
Where would you select a gene for recombinant gene expression?
Where would you select a gene for recombinant gene expression?
What is a distinguishing feature of expression plasmids in comparison to cloning plasmids?
What is a distinguishing feature of expression plasmids in comparison to cloning plasmids?
Which type of vector generally has low copy number per cell?
Which type of vector generally has low copy number per cell?
What is the main function of restriction enzymes in recombinant DNA technology?
What is the main function of restriction enzymes in recombinant DNA technology?
What was observed in the 1950s that led to the discovery of restriction enzymes?
What was observed in the 1950s that led to the discovery of restriction enzymes?
Which tag is available in pET plasmids for detection and purification of expressed proteins?
Which tag is available in pET plasmids for detection and purification of expressed proteins?
Which plasmid has a ColE1 origin of replication?
Which plasmid has a ColE1 origin of replication?
What is the role of ligases in recombinant DNA technology?
What is the role of ligases in recombinant DNA technology?
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