Enzymes Flashcards
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Enzymes Flashcards

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Questions and Answers

What is the function of an enzyme?

  • To prevent reactions from occurring
  • To slow down chemical reactions
  • To serve as a main energy source
  • To speed up chemical reactions (correct)
  • What is a substrate?

    A compound or molecule that an enzyme binds to and on which it acts.

    What forms when an enzyme binds to its substrate?

    Enzyme-substrate complex

    What is a product in the context of enzymatic reactions?

    <p>A substance formed as a result of a chemical reaction.</p> Signup and view all the answers

    What is the active site of an enzyme?

    <p>Region on the surface of an enzyme where the substrate binds and where the reaction occurs.</p> Signup and view all the answers

    What is activation energy?

    <p>Energy needed to get a reaction started.</p> Signup and view all the answers

    What does denaturation refer to?

    <p>A process in which a protein unravels, losing its specific structure and hence function.</p> Signup and view all the answers

    Define a metabolic pathway.

    <p>A series of chemical reactions that builds or breaks down complex molecules.</p> Signup and view all the answers

    What is an anabolic pathway?

    <p>A process in which large molecules are built from small molecules.</p> Signup and view all the answers

    What characterizes a catabolic pathway?

    <p>A process in which large molecules are broken down into simpler, smaller molecules.</p> Signup and view all the answers

    What do endergonic reactions require?

    <p>Input of energy</p> Signup and view all the answers

    What is ATP?

    <p>Main energy source used in cells</p> Signup and view all the answers

    What is a catalyst?

    <p>A chemical substance that selectively increases the rate of a reaction without being consumed.</p> Signup and view all the answers

    What is a biological catalyst?

    <p>Something that increases the rate of biological processes, mostly within cells.</p> Signup and view all the answers

    Explain the lock and key hypothesis.

    <p>The model of enzyme action where the substrate fits exactly into the active site on the enzyme.</p> Signup and view all the answers

    What does the induced fit hypothesis propose?

    <p>The active site of the enzyme may alter its conformation to better fit the substrate.</p> Signup and view all the answers

    Define optimum activity.

    <p>When an enzyme converts substrate to product at the fastest possible rate under ideal conditions.</p> Signup and view all the answers

    What is optimum pH?

    <p>The pH at which an enzyme works best.</p> Signup and view all the answers

    What is optimum temperature?

    <p>The temperature at which an enzyme works best.</p> Signup and view all the answers

    What is a limiting factor?

    <p>A factor that is in short supply and stops the rate of reaction from being any higher.</p> Signup and view all the answers

    What does thermophilic mean?

    <p>'Heat loving' organisms adapted to high temperatures, generally above 60 degrees Celsius.</p> Signup and view all the answers

    What are cofactors?

    <p>Molecules required to enable an enzyme to function normally.</p> Signup and view all the answers

    Define coenzymes.

    <p>Small organic molecules, usually vitamins, that participate in enzymatic reactions.</p> Signup and view all the answers

    What is an apoenzyme?

    <p>An enzyme requiring a cofactor to become catalytically active.</p> Signup and view all the answers

    What characterizes a holoenzyme?

    <p>An active enzyme containing all necessary cofactors.</p> Signup and view all the answers

    What is a prosthetic group?

    <p>A non-protein organic molecule that forms a permanent part of a functioning protein.</p> Signup and view all the answers

    Define competitive inhibition.

    <p>The process where a substance reduces enzyme activity by entering the active site instead of the substrate.</p> Signup and view all the answers

    What is non-competitive inhibition?

    <p>A process where a substance reduces enzyme activity by binding to a site other than the active site.</p> Signup and view all the answers

    What is catalase?

    <p>A common enzyme that catalyzes the decomposition of hydrogen peroxide to water and oxygen.</p> Signup and view all the answers

    What is an independent variable?

    <p>The experimental factor that is manipulated.</p> Signup and view all the answers

    What is a dependent variable?

    <p>The experimental factor that is being measured.</p> Signup and view all the answers

    What are intracellular enzymes?

    <p>Enzymes that act inside cells.</p> Signup and view all the answers

    What are extracellular enzymes?

    <p>Enzymes that influence reactions outside the cell they are produced in.</p> Signup and view all the answers

    What does specificity mean in the context of enzymes?

    <p>The term used to describe that an enzyme will only catalyze one type of reaction.</p> Signup and view all the answers

    What does substrate concentration refer to?

    <p>How much substrate is in a solution.</p> Signup and view all the answers

    What does enzyme concentration refer to?

    <p>How much enzyme is in a solution.</p> Signup and view all the answers

    Study Notes

    Enzymes Overview

    • Enzymes are proteins that act as catalysts, speeding up chemical reactions by lowering activation energy without being consumed.
    • Each enzyme binds to a specific substrate, forming an enzyme-substrate complex during the reaction.

    Key Terms

    • Substrate: The molecule an enzyme acts upon.
    • Product: The substance produced after the chemical reaction.
    • Active Site: The portion of the enzyme where the substrate binds and the reaction occurs.

    Reaction Dynamics

    • Activation Energy: The energy needed to initiate a reaction.
    • Denaturation: The process where proteins lose their functional structure due to extreme pH, salt concentration, or temperature changes.

    Metabolic Pathways

    • Metabolic Pathway: Series of enzymatic reactions that build (anabolic) or break down (catabolic) molecules.
    • Anabolic Pathway: Involves constructing larger molecules from smaller units.
    • Catabolic Pathway: Involves breaking down larger molecules into smaller components.

    Energy and Reactions

    • Endergonic Reactions: Require energy input to proceed.
    • Exergonic Reactions: Release energy during the process.

    Energy Carrier

    • ATP (Adenosine Triphosphate): Major energy currency in cells used in many metabolic processes, including enzyme-driven reactions.

    Catalyst Functions

    • Catalyst: A substance that accelerates a chemical reaction without undergoing permanent chemical change.
    • Biological Catalyst: Often refers to enzymes that enhance biological reactions.

    Enzyme Action Models

    • Lock and Key Hypothesis: Suggests that a substrate fits perfectly into the enzyme's active site.
    • Induced Fit Hypothesis: Proposes that the enzyme's active site adjusts to better fit the substrate upon binding.

    Optimal Conditions

    • Optimum Activity: Ideal conditions for maximum enzyme efficiency.
    • Optimum pH: The specific pH level at which an enzyme operates best.
    • Optimum Temperature: The best temperature for enzyme activity to maximize reaction rates.

    Factors Influencing Enzymatic Activity

    • Limiting Factor: A scarce resource that can slow down the rate of reaction.
    • Cofactors: Essential molecules (metal ions or organic coenzymes) required for enzyme function.
    • Coenzymes: Small organic molecules, often derived from vitamins, that assist enzyme catalysis.

    Enzyme Structure

    • Apoenzyme: The protein component of an enzyme that needs a cofactor to be active.
    • Holoenzyme: The fully active form of an enzyme that contains its cofactor(s).
    • Prosthetic Group: A non-protein component that permanently attaches to and is essential for the enzyme's function.

    Inhibition Mechanisms

    • Competitive Inhibition: Occurs when a substance mimics the substrate's structure and competes for the active site.
    • Non-competitive Inhibition: Involves a substance binding to a different location on the enzyme, altering its shape and function.

    Types of Enzymes

    • Catalase: An enzyme found in living organisms that breaks down hydrogen peroxide into water and oxygen.
    • Intracellular Enzymes: Enzymes that function within cells.
    • Extracellular Enzymes: Enzymes that act outside their production site, e.g., digestive enzymes.

    Reaction Parameters

    • Specificity: Refers to the ability of an enzyme to catalyze only one type of reaction.
    • Substrate Concentration: The concentration level of substrates available for enzyme reactions.
    • Enzyme Concentration: The amount of enzyme present in a solution, affecting reaction rates.

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    Explore the key concepts of enzymes with this set of flashcards. Learn about the role of enzymes, substrates, and the enzyme-substrate complex. Perfect for students looking to enhance their understanding of biochemistry.

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