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Enzymes as Bioreceptor Molecules in Biosensor Applications

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Enzymes as Bioreceptor Molecules in Biosensor Applications

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Questions and Answers

What is the main reason enzymes are chosen for biosensors?

Enzymes are natural proteins that catalyze a specific substrate (correct)

Enzymes are easily integrated with different transduction mechanisms

Enzymes are cheap to produce

Enzymes are highly reactive and can detect a wide range of analytes

How do enzymes recognize their target analyte in a biosensor?

Enzymes recognize their target analyte through random collisions

Enzymes recognize their target analyte through a lock-and-key mechanism (correct)

Enzymes recognize their target analyte through a complex signaling pathway

Enzymes recognize their target analyte through non-specific interactions

What are the main advantages of using enzymes as bioreceptors in biosensors?

Enzymes are cheap, fast, and can detect a wide range of analytes

Enzymes are easy to immobilize, stable, and have a long shelf-life

Enzymes are highly selective, sensitive, and can be combined with different transduction mechanisms (correct)

Enzymes are natural, renewable, and environmentally friendly

What are the three main mechanisms of operation of enzyme-based bioreceptors in biosensors?

Conversion of the analyte, detection of analyte inhibition/activation, and modification of enzyme properties Signup and view all the answers

How do enzymes compare to chemical reactions in biosensors?

Enzymes are more selective, sensitive, and faster acting than chemical reactions Signup and view all the answers

What is the primary advantage of using enzymes as bioreceptors in biosensors?

They have specific binding capabilities and catalytic activity. Signup and view all the answers

Which of the following statements about enzyme structure is true?

Some enzymes require only their amino acid residues for activity. Signup and view all the answers

What happens to an enzyme's catalytic activity if its native protein conformation is disrupted?

The catalytic activity is destroyed. Signup and view all the answers

What is the purpose of using enzyme-coupled receptors in biosensors?

To modify the recognition mechanisms of the biosensor. Signup and view all the answers

Which of the following statements about enzyme cascades is true?

They are often used to enhance the enzymatic activity. Signup and view all the answers

Which of the following components can act as a cofactor for an enzyme?

Inorganic ions like $Fe^{2+}$, $Mg^{2+}$, $Mn^{2+}$, or $Zn^{2+}$ Signup and view all the answers