Podcast
Questions and Answers
What is the function of helicase?
What is the function of helicase?
Breaks or melts the hydrogen bonds that hold the two halves of the double helix together
What does topoisomerase do?
What does topoisomerase do?
Breaks and reseals the DNA backbone to allow the backbone to release tension caused by twisting
What is the role of primase in DNA replication?
What is the role of primase in DNA replication?
Also called RNA polymerase, puts down small pieces of RNA called primers.
What does DNA polymerase do?
What does DNA polymerase do?
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What is the function of DNA polymerase III?
What is the function of DNA polymerase III?
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What does DNA polymerase I do?
What does DNA polymerase I do?
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What is the role of DNA ligase?
What is the role of DNA ligase?
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Study Notes
Enzymes Involved in DNA Replication
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Helicase: Unwinds DNA double helix by breaking hydrogen bonds between base pairs, essential for opening the DNA strands.
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Topoisomerase: Relieves supercoiling and tension in the DNA helix by cutting and resealing the backbone, preventing tangling during replication.
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Primase: Functions as RNA polymerase to synthesize short RNA primers, which provide starting points for DNA replication.
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DNA Polymerase: Enzyme responsible for synthesizing new DNA strands by adding nucleotides complementary to the template strand; two types play distinct roles.
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DNA Polymerase III: Primary enzyme in DNA replication; reads existing DNA strands to create new complementary strands according to base pairing rules.
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DNA Polymerase I: Removes RNA primers and replaces them with DNA nucleotides, ensuring the final DNA molecule contains only DNA sequences.
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DNA Ligase: Fills in gaps between adjacent nucleotides by forming covalent bonds, effectively sealing breaks and completing the DNA strand continuity.
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Description
This quiz covers the key enzymes involved in DNA replication, including Helicase, Topoisomerase, Primase, and DNA Polymerases. Test your knowledge on how these enzymes function and their roles in the replication process. Understand the complexities of DNA mechanics and their significance in cell division.
