Enzymes and Their Mechanisms
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Questions and Answers

Which characteristic is true of fatty acid tails?

  • They contain many polar groups.
  • They are hydrophilic.
  • They are long hydrocarbon chains with hydrophobic properties. (correct)
  • They are short hydrocarbon chains.
  • A fatty acid with multiple double bonds is classified as:

  • Trans-unsaturated.
  • Polyunsaturated. (correct)
  • Saturated.
  • Monounsaturated.
  • What type of lipid has a polar phosphate head group and nonpolar fatty acid tails?

  • Triglyceride.
  • Phospholipid. (correct)
  • Wax.
  • Glycolipid.
  • Which factor does NOT directly affect membrane fluidity?

    <p>Number of peptide bonds. (B)</p> Signup and view all the answers

    What is the main function of high density lipoproteins (HDLs)?

    <p>Deliver cholesterol to the liver for metabolism. (B)</p> Signup and view all the answers

    Which class of lipids is primarily used as hydrophobic coatings?

    <p>Waxes. (D)</p> Signup and view all the answers

    What type of bond connects two monosaccharides to form a disaccharide?

    <p>Glycosidic bond (A)</p> Signup and view all the answers

    Which type of lipids are involved in signal transduction, structural support, and cell recognition?

    <p>Sphingolipids. (B)</p> Signup and view all the answers

    What defines an element?

    <p>It is a pure substance with specific chemical and physical properties that cannot be broken down. (D)</p> Signup and view all the answers

    Which of these best describes a polymer?

    <p>A substance made of many monomers. (C)</p> Signup and view all the answers

    What is the primary difference between ribose and deoxyribose sugars?

    <p>Ribose has two hydroxyl groups, whereas deoxyribose has one. (B)</p> Signup and view all the answers

    What type of reaction is responsible for the formation of phosphodiester bonds between nucleotides?

    <p>Dehydration (condensation) (D)</p> Signup and view all the answers

    Which of the following is true about intermolecular forces?

    <p>They act between different molecules. (C)</p> Signup and view all the answers

    Which of these statements accurately describes the purpose of tRNA?

    <p>It transfers amino acids to ribosome enzymes. (B)</p> Signup and view all the answers

    Given the formula for monosaccharides $(CH_2O)_n$, how many carbon atoms are present in a monosaccharide with n=5?

    <p>5 (C)</p> Signup and view all the answers

    What process is used to break down polymers into individual monomers using water?

    <p>Hydrolysis (A)</p> Signup and view all the answers

    What type of bonds primarily hold together starch and glycogen polysaccharides?

    <p>α-glycosidic bonds (B)</p> Signup and view all the answers

    Which protein structure is characterized by the specific sequence of amino acids?

    <p>Primary structure (A)</p> Signup and view all the answers

    What is the role of peptide bonds in proteins?

    <p>To link amino acids together (B)</p> Signup and view all the answers

    Which statement is correct regarding the role of a catalyst in a chemical reaction?

    <p>It increases the reaction rate without altering equilibrium. (D)</p> Signup and view all the answers

    Which polysaccharide serves as a structural component in plant cells?

    <p>Cellulose (C)</p> Signup and view all the answers

    What is a key characteristic of an enzyme's active site?

    <p>It binds very specific substrates and may slightly change shape to fit the substrate. (B)</p> Signup and view all the answers

    What happens to a protein when it denatures?

    <p>It loses its higher level structure (D)</p> Signup and view all the answers

    Which of these best describes a holoenzyme?

    <p>An enzyme bound to its appropriate cofactor. (B)</p> Signup and view all the answers

    What is primarily accomplished by a phosphatase enzyme?

    <p>Cleaves phosphate groups off of a substrate molecule. (C)</p> Signup and view all the answers

    How does a negative feedback loop affect enzyme activity?

    <p>It decreases the reaction rate. (A)</p> Signup and view all the answers

    In competitive inhibition, what happens when an inhibitor binds to the enzyme?

    <p>The inhibitor binds to the active site, preventing substrate binding. (A)</p> Signup and view all the answers

    If a reaction is subject to noncompetitive inhibition, how do $K_M$ and $V_{max}$ change?

    <p>$K_M$ remains unchanged, and $V_{max}$ decreases. (A)</p> Signup and view all the answers

    What effect does competitive inhibition have on $K_M$ and $V_{max}$ of an enzymatic reaction?

    <p>$K_M$ increases and $V_{max}$ remains unchanged. (C)</p> Signup and view all the answers

    Flashcards

    Enzymes

    Biological catalysts that speed up reactions without being consumed.

    Active Site

    The specific region on an enzyme where the substrate binds.

    Ribozyme

    A type of enzyme that is made of RNA instead of protein.

    Cofactors

    Molecules that help enzymes function by binding to them.

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    Holoenzyme

    An enzyme that is bound to its appropriate cofactor, ready to perform its function.

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    Apoenzyme

    An enzyme that is missing its cofactor and is inactive.

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    Inhibitor

    A molecule that binds to an enzyme's allosteric site, altering its shape and activity.

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    Vmax

    The maximum velocity of a reaction catalyzed by an enzyme.

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    What are polysaccharides?

    Polysaccharides are complex carbohydrates made up of many simple sugar units (monosaccharides) linked together by glycosidic bonds.

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    What is starch?

    Starch is a type of polysaccharide made up of glucose units, used by plants for energy storage. The glucose units are linked by alpha-glycosidic bonds.

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    What is glycogen?

    Glycogen is another type of polysaccharide made up of glucose units, used by animals for energy storage. The glucose units are linked by alpha-glycosidic bonds.

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    What is cellulose?

    Cellulose is a structural polysaccharide found in plant cell walls, providing support and strength.

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    What are amino acids?

    Amino acids are the building blocks of proteins. They are composed of carbon, hydrogen, nitrogen, and oxygen.

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    What is an atom?

    The smallest unit of matter that retains the chemical properties of an element.

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    What is a molecule?

    Two or more atoms joined together.

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    What are intramolecular forces?

    Attractive forces that act on atoms within a molecule.

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    What are intermolecular forces?

    Attractive forces between molecules.

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    What is a monomer?

    Single molecules that can polymerize.

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    What is polymerization?

    The linking of several monomer units.

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    What is a polymer?

    Substances made of many monomers.

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    What is a dehydration reaction?

    A mode of polymerization that releases water.

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    Fatty Acid

    A long chain of carbons and hydrogens with a carboxyl group at one end. They are hydrophobic due to their nonpolar nature.

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    Unsaturated Fatty Acid

    A type of lipid that has at least one double bond within its hydrocarbon chain. This makes the chain kinked and less tightly packed.

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    Saturated Fatty Acid

    A type of lipid that has no double bonds in its hydrocarbon chain. This makes the chain straight and tightly packed.

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    Triglyceride

    A type of lipid composed of a glycerol backbone attached to three fatty acid tails.

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    Phospholipid

    A type of lipid that has a hydrophilic head group and two hydrophobic fatty acid tails. These lipids spontaneously form bilayers in aqueous solutions.

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    Membrane Fluidity

    The ability of a lipid bilayer to maintain its fluidity, allowing for movement of molecules within the membrane.

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    Cholesterol

    An amphipathic lipid that is a major component of cell membranes. It helps regulate membrane fluidity and is also a precursor to steroid hormones.

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    Lipoprotein

    A type of lipid that carries cholesterol and other lipids through the bloodstream. It can be classified into high-density (HDL) and low-density (LDL) lipoproteins.

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    Study Notes

    Enzymes

    • Catalysts increase reaction rates without affecting equilibrium, reaction direction, or spontaneity.
    • Enzymes are biological catalysts, mostly proteins.
    • Enzymes have active sites that bind specific substrates to increase reaction rate.
    • Active sites exhibit "induced fit," slightly changing shape to accommodate the substrate.
    • Ribozymes are non-protein enzymes made of RNA.
    • Cofactors are molecules that enhance enzyme function.
    • Holoenzymes are enzymes with their cofactors bound.
    • Apoenzymes are enzymes without their cofactors.
    • Enzymes can catalyze reactions through substrate approach, presence of acids/bases, and electrostatic interactions.
    • Phosphatases remove phosphate groups from substrates.
    • Phosphorylases add phosphates to substrates via bond breakage.
    • Kinases transfer phosphates from ATP to a substrate.

    Enzyme Inhibition and Kinetics

    • Enzyme activity alteration impacts reaction rates.
    • Substrate binding to active or allosteric sites impacts manipulation.
    • Positive feedback loops increase reaction.
    • Negative feedback loops decrease reaction.
    • Molecules bind to allosteric sites influencing enzyme function.
    • Competitive inhibitors compete with substrates for the active site.
    • Noncompetitive inhibitors bind to allosteric site, altering active site conformation.
    • Enzyme kinetics tracks reaction velocities against substrate concentration.
    • Vmax is the maximum reaction velocity.
    • Km is substrate concentration at half Vmax.
    • Competitive inhibition increases Km, preserving Vmax.
    • Non-competitive inhibition decreases Vmax, preserving Km.

    Lipids

    • Lipids primarily consist of carbon, hydrogen, and oxygen.
    • Fatty acid tails are hydrophobic hydrocarbon chains.
    • Lipids can be hydrophobic, hydrophilic, or amphipathic.
    • Saturated fatty acids lack double bonds, whereas unsaturated fatty acids have one or more double bonds.
    • Monounsaturated fatty acids have one double bond, while polyunsaturated fatty acids have multiple double bonds.
    • Cis and trans configurations affect lipid packing.
    • Glycerol backbones hold fatty acids in triglycerides.
    • Triglycerides are mostly hydrophobic.
    • Phospholipids are amphipathic, with a polar head and nonpolar tails.
    • Phospholipids form bilayers in aqueous solutions.

    Lipid Subtypes

    • Membrane fluidity depends on temperature, cholesterol, and fatty acid unsaturation.
    • Cholesterol is a crucial component in steroid hormones, vitamin D, and bile acids.
    • Lipoproteins transport cholesterol and proteins in the blood.
    • Low-density lipoproteins deposit cholesterol in tissues.
    • High-density lipoproteins deliver cholesterol to the liver.
    • Waxes are simple hydrophobic protective coatings.
    • Carotenoids are long-chain lipids found in pigments.
    • Sphingolipids support cell signaling, structure, and recognition.
    • Glycolipids are fatty acids attached to carbohydrates, crucial for cell membranes.

    Biological Chemistry

    • Matter occupies space and possesses mass.
    • Elements are pure substances with unique chemical and physical properties.
    • Atoms are the fundamental units of matter.
    • Molecules are formed when two or more atoms bind.
    • Intramolecular forces bond atoms within a molecule.
    • Intermolecular forces bond molecules together.
    • Monomers are single molecules that combine to form polymers.
    • Polymerization bonds monomers into polymers.
    • Dehydration (condensation) reactions link monomers, releasing water.
    • Depolymerization breaks polymers into monomers.
    • Hydrolysis uses water to break polymer bonds.

    Nucleic Acids

    • Nucleic acids (DNA and RNA) are built from nucleotides.
    • Nucleotides consist of a sugar, a nitrogenous base, and phosphate groups.
    • RNA molecules are typically single-stranded.
    • Ribose sugar is the component of RNA.
    • DNA molecules are typically double-stranded.
    • Deoxyribose sugar is the component of DNA
    • Phosphodiester bonds connect nucleotides together in a chain.
    • The backbone of nucleic acids is formed by sugars and phosphates.
    • Nucleotides join to create a nucleic acid chain.
    • mRNA carries genetic information for protein synthesis.
    • tRNA transfers amino acids during protein synthesis.
    • rRNA is a component of ribosomes, sites of protein synthesis.
    • miRNA and dsRNA regulate gene expression.

    Carbohydrates

    • Carbohydrates are used for energy and structural support.
    • Carbohydrates contain carbon, hydrogen, and oxygen atoms.
    • Monosaccharides are simple carbohydrates (e.g., ribose, fructose, glucose).
    • Disaccharides are formed by linking two monosaccharides through glycosidic bonds.
    • Polysaccharides are complex carbohydrates with many monosaccharides linked through glycosidic bonds.
    • Starch is a plant-based storage polysaccharide.
    • Glycogen is an animal-based storage polysaccharide.
    • Cellulose is a structural polysaccharide found in plant cell walls.

    Protein Structure

    • Proteins are composed of amino acids linked by peptide bonds.
    • There are 20 different amino acids in proteins.
    • Primary structure is the linear sequence of amino acids.
    • Secondary structure involves local folding patterns (alpha-helices, beta-sheets).
    • Tertiary structure is the three-dimensional shape of a single polypeptide chain.
    • Quaternary structure involves the arrangement of multiple polypeptide chains to form a protein complex.
    • Protein denaturation disrupts higher-level structure, affecting function.
    • Proteins have diverse functions (e.g., enzymes, hormones, structural components).

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    Description

    This quiz covers the fundamental concepts of enzymes, their roles as biological catalysts, and the various mechanisms of enzyme action. Topics include active sites, co-factors, and enzyme kinetics. Test your understanding of these essential biochemical processes!

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