Enzyme Source Recognition Quiz
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Questions and Answers

What type of DNA ends are produced when some endonucleases cut both strands of a DNA molecule at points directly opposite each other?

  • Sticky ends
  • Overhanging ends
  • Blunt ends (correct)
  • Single-stranded ends

    • Which enzyme is used to join DNA fragments with 'blunt ends' together?

  • DNA helicase
  • DNA ligase (correct)
  • Endonuclease
  • DNA polymerase

    • In 'blunt end' ligation, how are the DNA fragments joined together?

  • Through the use of RNA ligase
  • Through DNA polymerase activity
  • Directly using DNA ligase (correct)
  • With complementary base pairing

    • Which type of DNA ends allow for more control over the orientation of the resultant insertion?

    <p>Sticky ends</p> Signup and view all the answers

    When identifying restriction sites, why is it important to consider the direction of the DNA strands?

    <p>To ensure the correct base pairs are recognized</p> Signup and view all the answers

    What is the main function of endonucleases in genetic engineering?

    <p>Introduce breaks at specific sites in DNA molecules</p> Signup and view all the answers

    What determines how frequently a restriction enzyme cuts a random sequence of DNA?

    <p>The number of base pairs in the restriction site</p> Signup and view all the answers

    What is the significance of recognition sequences for restriction enzymes?

    <p>They indicate where a restriction enzyme can cut double-stranded DNA</p> Signup and view all the answers

    How do blunt ends and sticky ends differ in the context of DNA manipulation?

    <p>Sticky ends have an overhanging sequence that can base pair with complementary ends</p> Signup and view all the answers

    What is the role of DNA ligase in gene cloning procedures?

    <p>To join DNA fragments by forming phosphodiester bonds</p> Signup and view all the answers

    In the context of endonucleases, what do recognition sites refer to?

    <p>Specific sequences where endonucleases cut double-stranded DNA</p> Signup and view all the answers

    Why is it important for researchers to know the recognition sites of restriction enzymes?

    <p>To predict where a restriction enzyme will cut DNA molecules</p> Signup and view all the answers

    What is the significance of the symbol * in the recognition sequences of restriction enzymes?

    <p>It represents the position of the cut site.</p> Signup and view all the answers

    When adding EcoRI to a solution containing the specified double-stranded DNA, what is the expected outcome?

    <p>Two fragments of double-stranded DNA, each with a sticky end.</p> Signup and view all the answers

    Which statement correctly describes DNA fragments resulting from HindIII digestion of a DNA molecule?

    <p>Two fragments of double-stranded DNA, each with blunt ends.</p> Signup and view all the answers

    Which enzyme will cut the specified piece of DNA: 5’ C T T A A G C T T C C A A A T T A C C G A 3’?

    <p>HindIII only</p> Signup and view all the answers

    How do enzymes like EcoRI create sticky ends in DNA?

    <p>By creating 5' overhangs in the cut sites.</p> Signup and view all the answers

    What is the function of DNA ligase in genetic engineering processes involving restriction enzymes?

    <p>To join DNA fragments by catalyzing phosphodiester bond formation.</p> Signup and view all the answers

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