Enzyme Source Recognition Quiz

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Questions and Answers

What type of DNA ends are produced when some endonucleases cut both strands of a DNA molecule at points directly opposite each other?

  • Sticky ends
  • Overhanging ends
  • Blunt ends (correct)
  • Single-stranded ends

Which enzyme is used to join DNA fragments with 'blunt ends' together?

  • DNA helicase
  • DNA ligase (correct)
  • Endonuclease
  • DNA polymerase

In 'blunt end' ligation, how are the DNA fragments joined together?

  • Through the use of RNA ligase
  • Through DNA polymerase activity
  • Directly using DNA ligase (correct)
  • With complementary base pairing

Which type of DNA ends allow for more control over the orientation of the resultant insertion?

<p>Sticky ends (C)</p> Signup and view all the answers

When identifying restriction sites, why is it important to consider the direction of the DNA strands?

<p>To ensure the correct base pairs are recognized (A)</p> Signup and view all the answers

What is the main function of endonucleases in genetic engineering?

<p>Introduce breaks at specific sites in DNA molecules (D)</p> Signup and view all the answers

What determines how frequently a restriction enzyme cuts a random sequence of DNA?

<p>The number of base pairs in the restriction site (D)</p> Signup and view all the answers

What is the significance of recognition sequences for restriction enzymes?

<p>They indicate where a restriction enzyme can cut double-stranded DNA (D)</p> Signup and view all the answers

How do blunt ends and sticky ends differ in the context of DNA manipulation?

<p>Sticky ends have an overhanging sequence that can base pair with complementary ends (B)</p> Signup and view all the answers

What is the role of DNA ligase in gene cloning procedures?

<p>To join DNA fragments by forming phosphodiester bonds (D)</p> Signup and view all the answers

In the context of endonucleases, what do recognition sites refer to?

<p>Specific sequences where endonucleases cut double-stranded DNA (A)</p> Signup and view all the answers

Why is it important for researchers to know the recognition sites of restriction enzymes?

<p>To predict where a restriction enzyme will cut DNA molecules (D)</p> Signup and view all the answers

What is the significance of the symbol * in the recognition sequences of restriction enzymes?

<p>It represents the position of the cut site. (B)</p> Signup and view all the answers

When adding EcoRI to a solution containing the specified double-stranded DNA, what is the expected outcome?

<p>Two fragments of double-stranded DNA, each with a sticky end. (A)</p> Signup and view all the answers

Which statement correctly describes DNA fragments resulting from HindIII digestion of a DNA molecule?

<p>Two fragments of double-stranded DNA, each with blunt ends. (D)</p> Signup and view all the answers

Which enzyme will cut the specified piece of DNA: 5’ C T T A A G C T T C C A A A T T A C C G A 3’?

<p>HindIII only (B)</p> Signup and view all the answers

How do enzymes like EcoRI create sticky ends in DNA?

<p>By creating 5' overhangs in the cut sites. (A)</p> Signup and view all the answers

What is the function of DNA ligase in genetic engineering processes involving restriction enzymes?

<p>To join DNA fragments by catalyzing phosphodiester bond formation. (B)</p> Signup and view all the answers

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