18 Questions
What type of DNA ends are produced when some endonucleases cut both strands of a DNA molecule at points directly opposite each other?
Blunt ends
Which enzyme is used to join DNA fragments with 'blunt ends' together?
DNA ligase
In 'blunt end' ligation, how are the DNA fragments joined together?
Directly using DNA ligase
Which type of DNA ends allow for more control over the orientation of the resultant insertion?
Sticky ends
When identifying restriction sites, why is it important to consider the direction of the DNA strands?
To ensure the correct base pairs are recognized
What is the main function of endonucleases in genetic engineering?
Introduce breaks at specific sites in DNA molecules
What determines how frequently a restriction enzyme cuts a random sequence of DNA?
The number of base pairs in the restriction site
What is the significance of recognition sequences for restriction enzymes?
They indicate where a restriction enzyme can cut double-stranded DNA
How do blunt ends and sticky ends differ in the context of DNA manipulation?
Sticky ends have an overhanging sequence that can base pair with complementary ends
What is the role of DNA ligase in gene cloning procedures?
To join DNA fragments by forming phosphodiester bonds
In the context of endonucleases, what do recognition sites refer to?
Specific sequences where endonucleases cut double-stranded DNA
Why is it important for researchers to know the recognition sites of restriction enzymes?
To predict where a restriction enzyme will cut DNA molecules
What is the significance of the symbol * in the recognition sequences of restriction enzymes?
It represents the position of the cut site.
When adding EcoRI to a solution containing the specified double-stranded DNA, what is the expected outcome?
Two fragments of double-stranded DNA, each with a sticky end.
Which statement correctly describes DNA fragments resulting from HindIII digestion of a DNA molecule?
Two fragments of double-stranded DNA, each with blunt ends.
Which enzyme will cut the specified piece of DNA: 5’ C T T A A G C T T C C A A A T T A C C G A 3’?
HindIII only
How do enzymes like EcoRI create sticky ends in DNA?
By creating 5' overhangs in the cut sites.
What is the function of DNA ligase in genetic engineering processes involving restriction enzymes?
To join DNA fragments by catalyzing phosphodiester bond formation.
Test your knowledge on enzyme recognition sites for AluI, BamHI, EcoRI, HindIII, KpnI, NotI, PstI, SacI, and more from various sources like Arthrobacter luteus, Bacillus amyloliquefaciens, Escherichia coli, Hemophilus influenza Rd, Klebsiella pneumoniae, Providencia stuartii, and Streptomyces achromogenes.
Make Your Own Quizzes and Flashcards
Convert your notes into interactive study material.
Get started for free
