Biochem 7: Enzyme Kinetics

Which type of enzyme inhibition occurs when the inhibitor binds to the enzyme-substrate complex?

In uncompetitive inhibition, is the Vmax lower or higher in the presence of the inhibitor?

Does the affinity of the enzyme appear to go up or down in the presence of the uncompetitive inhibitor?

Which type of enzyme inhibition occurs when the inhibitor can bind to both the enzyme and the enzyme-substrate complex?

In competitive inhibition, is the Vmax lower or higher in the presence of the inhibitor?

Does the affinity of the enzyme appear to go up or down in the presence of the competitive inhibitor?

Which type of enzyme inhibition occurs when the inhibitor can bind to both the enzyme and the enzyme-substrate complex, but at different binding sites?

In noncompetitive inhibition, is the Vmax lower or higher in the presence of the inhibitor?

Does the affinity of the enzyme appear to go up or down in the presence of the noncompetitive inhibitor?

Which type of enzyme inhibition occurs when the inhibitor forms a covalent bond with the active site of the enzyme?

Which of the following is true about Michaelis-Menten kinetics?

What is the role of glucokinase in the liver?

What is the difference between glucokinase and hexokinase in terms of Vmax?

What is the difference between glucokinase and hexokinase in terms of Km?

What is the purpose of a Lineweaver-Burk plot?

Which of the following is an example of reversible enzyme inhibition?

What is the graph of an allosteric enzyme in the presence of an inhibitor?

What is the ratio that determines enzyme efficiency?

Which of the following is true about first order reactions?

Which of the following is true about zero order reactions?

Which enzyme is active even during fasting?

What enzyme doesn't become active until after a high-carbohydrate meal?

What is the main role of glucokinase and hexokinase?

What does a higher Km tell you about the affinity of glucokinase for glucose, compared to the affinity of hexokinase for glucose?

What pathway does hexokinase feed into if it can't do glycogenesis?

Why is hexokinase inhibited by glucose-6-P, but glucokinase is not?

What is the general purpose of very efficient enzymes?

Which is a more efficient enzyme: one with a larger or smaller value for Kcat/Km?

What are some applications of enzyme inhibition?

What type of reversible inhibition is exemplified by methotrexate?

Which of the following is an example of enzyme regulation via reversible covalent modification?

Which of the following is FALSE regarding enzymes?

Which of the following is an example of enzyme regulation via irreversible covalent modification?

What kind of bonds form the primary structure of a protein?

When a heavy metal such as lead (Pb+2) interacts with bonds within a protein, it can denature the protein. Which of the following bonds are vulnerable to this type of denaturation?

What important piece of information can be gained from Michaelis Menten kinetics?

Which of the following characteristics indicates the highest enzyme efficiency?

Which of the following is true of any pseudo-first order reaction?