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Questions and Answers
Enzyme activity is also regulated by ______ inhibition, where the end product of a metabolic pathway inhibits an enzyme at the start of the pathway, preventing overproduction.
Enzyme activity is also regulated by ______ inhibition, where the end product of a metabolic pathway inhibits an enzyme at the start of the pathway, preventing overproduction.
feedback
My results show that as the concentrations of copper nitrate solution decrease from 100% to 20% the volume of foam increases by ______.
My results show that as the concentrations of copper nitrate solution decrease from 100% to 20% the volume of foam increases by ______.
The results from my experiment, when compared to data from an internet source, show similar trends with increasing inhibitor ______.
The results from my experiment, when compared to data from an internet source, show similar trends with increasing inhibitor ______.
concentrations
As the inhibitor (copper nitrate solution 0.6%) concentration is decreased the ______ increases.
As the inhibitor (copper nitrate solution 0.6%) concentration is decreased the ______ increases.
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I used ______ to measure the volumes of reactants.
I used ______ to measure the volumes of reactants.
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Enzymes are ______ that control metabolic pathways in cells.
Enzymes are ______ that control metabolic pathways in cells.
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The experiment investigated the effect of ______ concentration on enzyme activity.
The experiment investigated the effect of ______ concentration on enzyme activity.
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The experiment used ______ nitrate as the inhibitor.
The experiment used ______ nitrate as the inhibitor.
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The volume of ______ gas produced was measured as an indicator of enzyme activity.
The volume of ______ gas produced was measured as an indicator of enzyme activity.
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Metabolic pathways can be ______ or catabolic.
Metabolic pathways can be ______ or catabolic.
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Enzymes have a specific ______ site where substrates bind.
Enzymes have a specific ______ site where substrates bind.
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In ______ inhibition, the inhibitor competes with the substrate for the active site.
In ______ inhibition, the inhibitor competes with the substrate for the active site.
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In non-competitive inhibition, the inhibitor binds to a different site on the ______, altering its shape.
In non-competitive inhibition, the inhibitor binds to a different site on the ______, altering its shape.
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Study Notes
Enzyme Inhibition
- Enzymes control metabolic pathways, crucial for energy production, biosynthesis and degradation.
- Metabolic pathways are either anabolic (building larger molecules) or catabolic (breaking down larger molecules).
- Enzymes have an active site where substrates bind, lowering activation energy to speed up reactions.
Types of Inhibition
-
Competitive Inhibition:
- Inhibitor has a similar shape to the substrate.
- Competes for the active site, preventing substrate binding.
- Enzyme activity decreases as inhibitor concentration increases.
- Increasing substrate concentration can overcome the effect.
-
Non-Competitive Inhibition:
- Inhibitor binds to a different site on the enzyme (not the active site).
- Changes the enzyme's shape, preventing substrate binding.
- Enzyme activity is permanently reduced, unaffected by substrate concentration.
Feedback Inhibition
- End product of a metabolic pathway inhibits an enzyme at the start of the pathway.
- Prevents overproduction of the end product.
Experiment Aim and Description
- Aim: Investigate the effect of inhibitor concentration on enzyme activity.
- Experiment: Different copper nitrate concentrations were used. Hydrogen peroxide, detergent and a liver cube were added. Oxygen volume was measured.
Results and Conclusion
- Copper nitrate concentration decrease led to increased enzyme activity (more foam).
- Results correlated with data from other sources, suggesting reliability.
Evaluation
- Experiment results were consistent across trials.
- Temperature was assumed constant, but could have been controlled better in a water bath.
- Syringes ensured consistent reagent volumes (improved measurements and validity).
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Description
This quiz covers the fundamental concepts of enzyme inhibition, including competitive, non-competitive, and feedback inhibition. Understand how enzymes interact with substrates and the implications of different types of inhibition on metabolic pathways. Test your knowledge of these critical biochemical processes.
