Enzyme Catalysis by Approximation Quiz
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Questions and Answers

What term describes a chemical species with an affinity for a positive charge and acts as an electron donor?

  • Coenzyme
  • Nucleophile (correct)
  • Prosthetic group
  • Electrophile

Which type of effector involves the substrate binding to the allosteric site in an enzyme?

  • Heterotropic effector
  • Homotropic effector (correct)
  • Cofactor
  • NAD+

What is the term for a small organic molecule required for enzyme activity?

  • Nucleophile
  • Coenzyme (correct)
  • Prosthetic group
  • Cofactor

In covalent catalysis, what does the enzyme form temporarily with the substrate to catalyze the reaction?

<p>Cosubstrate (C)</p> Signup and view all the answers

What term describes the released energy when weak interactions are formed between the enzyme and substrate, promoting specificity?

<p>Binding energy (C)</p> Signup and view all the answers

Which type of effector involves a ligand binding to the allosteric site that is different from the substrate?

<p>Heterotropic effector (C)</p> Signup and view all the answers

How is metal ion catalysis involved in nucleophile formation?

<p>Raising binding energy (D)</p> Signup and view all the answers

In NMP kinase, what is the purpose of having a divalent ion-bound nucleotide as the true substrate?

<p>Providing a specific conformation for binding (C)</p> Signup and view all the answers

How does proximity aid in catalysis in NMP kinase?

<p>Bringing NTP and NMP close together (D)</p> Signup and view all the answers

What is the role of the P-loop in NMP kinase activation?

<p>Triggering a lid domain closure (D)</p> Signup and view all the answers

What effect does metal ion catalysis have on serine proteases like chymotrypsin?

<p>Enhancing acid-base catalysis (B)</p> Signup and view all the answers

How does metal ion catalysis contribute to enzyme function in restriction endonucleases?

<p>Promotes bond cleavage by acting as a covalent catalyst (A)</p> Signup and view all the answers

What role does carbonic anhydrase play in the reaction of CO2 to HCO3-?

<p>Acts as a proton acceptor (B)</p> Signup and view all the answers

How does Zn2+ contribute to the reaction catalyzed by carbonic anhydrase?

<p>Lowers H2O's pKa (D)</p> Signup and view all the answers

What is the mechanism by which His 64 facilitates the reaction in carbonic anhydrase?

<p>Moves a proton from protein surface to H2O (A)</p> Signup and view all the answers

In carbonic anhydrase, what is the function of OH-?

<p>Attacks HCO3- (B)</p> Signup and view all the answers

What is the main impact of carbonic anhydrase on the speed of the reaction?

<p>Facilitates rapid conversion (D)</p> Signup and view all the answers

How does carbonic anhydrase promote the regeneration of its active site?

<p>Binds to Zn2+ again (C)</p> Signup and view all the answers

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