Enzyme Catalysis and Reaction Rate Quiz
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Questions and Answers

What is the catalytic strategy used by carbonic anhydrases?

  • Acid-Base Catalysis
  • Charge Complementarity
  • Covalent Catalysis
  • Approximation (correct)
  • What metal ion is universally used in the family prevalence of carbonic anhydrases?

  • Zinc (correct)
  • Cadmium
  • Cobalt
  • Iron
  • How many families of carbonic anhydrases have been discovered?

  • 5
  • 10
  • 15
  • 8 (correct)
  • What is the main physiological relevance of carbonic anhydrases?

    <p>pH regulation</p> Signup and view all the answers

    Which enzyme utilizes the catalytic strategy of Approximation?

    <p>Carbonic Anhydrase</p> Signup and view all the answers

    What is the steady state assumption?

    <p>The formation of the E·S complex occurs at the same rate as its loss</p> Signup and view all the answers

    What does the equilibrium constant Km represent?

    <p>The concentration of substrate needed to achieve half the maximum reaction rate</p> Signup and view all the answers

    In the equation Δ𝐸·𝑆 = 𝑘1 𝐸 𝑆 − 𝑘−1 𝐸·𝑆 − 𝑘2 𝐸·𝑆 = 0, what does Δ𝐸·𝑆 represent?

    <p>Change in the concentration of substrate-bound enzyme</p> Signup and view all the answers

    How does knowing [E]T and [E·S] help in understanding enzyme kinetics?

    <p>It enables determination of the rate of product formation</p> Signup and view all the answers

    What correction factor can be used to correct for osmolarity deviations?

    <p>Osmotic coefficient</p> Signup and view all the answers

    What is the main contributor to the osmolarity of the interstitial fluid and plasma?

    <p>NaCl ions</p> Signup and view all the answers

    What causes the slight difference between plasma and interstitial fluid osmolarity?

    <p>Osmotic effects of plasma proteins</p> Signup and view all the answers

    What maintains about 20 mm Hg greater pressure in the capillaries than in the surrounding interstitial spaces?

    <p>Plasma proteins</p> Signup and view all the answers

    What can cause a slight decrease in the osmotic activity of dissolved substances?

    <p>Interionic attraction</p> Signup and view all the answers

    What happens with small changes in the concentration of solutes in the extracellular fluid?

    <p>High osmotic pressures develop across the cell membrane</p> Signup and view all the answers

    About how much of the total osmolarity of intracellular fluid is due to potassium ions?

    <p>Half</p> Signup and view all the answers

    What is used to express corrected osmolar activities of plasma, interstitial fluid, and intracellular fluid?

    <p>(mOsm/L)</p> Signup and view all the answers

    What effect does urea have on intracellular volume under steady-state conditions?

    <p>It has little effect on intracellular volume</p> Signup and view all the answers

    Why is complete osmotic equilibrium not immediately achieved between the intracellular and extracellular compartments throughout the whole body?

    <p>Fluid needs to be transported by the blood to all tissues before equilibrium can occur</p> Signup and view all the answers

    What are some factors that can cause extracellular and intracellular volumes to change?

    <p>All of the above</p> Signup and view all the answers

    What is the timeframe within which differences in osmolarities between intracellular and extracellular compartments are usually corrected?

    <p>Minutes</p> Signup and view all the answers

    How does the transfer of fluid across the cell membrane contribute to the correction of differences in osmolarities?

    <p>It occurs rapidly, allowing for quick correction</p> Signup and view all the answers

    What is the primary reason for the delay in achieving complete osmotic equilibrium between the intracellular and extracellular compartments?

    <p>Fluid needs to be transported by the blood to all tissues</p> Signup and view all the answers

    What happens to the concentrations of highly permeating substances like urea in the intracellular and extracellular fluids over time?

    <p>They eventually become equal in the two compartments</p> Signup and view all the answers

    What is the immediate effect of highly permeating substances like urea on fluid volume between intracellular and extracellular fluids?

    <p>They cause transient shifts in fluid volume between the two compartments</p> Signup and view all the answers

    What is the relationship between pH and H+ concentration?

    <p>pH is inversely related to H+ concentration</p> Signup and view all the answers

    What is the normal pH of arterial blood?

    <p>7.4</p> Signup and view all the answers

    At what pH would a person be considered to have acidemia?

    <p>Below 6.8</p> Signup and view all the answers

    Why is it customary to express H+ concentration on a logarithm scale using pH units?

    <p>Because small numbers are cumbersome</p> Signup and view all the answers

    What would a high pH correspond to in terms of H+ concentration?

    <p>Low H+ concentration</p> Signup and view all the answers

    What is the pH of venous blood and interstitial fluids?

    <p>7.35</p> Signup and view all the answers

    What does a low pH correspond to in terms of H+ concentration?

    <p>High H+ concentration</p> Signup and view all the answers

    What does the formula pH=-log[H+] indicate about the relationship between pH and H+ concentration?

    <p>-log[H+] has an inverse relationship with pH</p> Signup and view all the answers

    What is the primary purpose of the body fluid buffers?

    <p>To neutralize the acids produced by the body</p> Signup and view all the answers

    What is the bicarbonate buffer system composed of?

    <p>A weak acid and a bicarbonate salt</p> Signup and view all the answers

    Where is carbonic anhydrase especially abundant in the body?

    <p>In the walls of the lung alveoli</p> Signup and view all the answers

    What happens to the daily production and ingestion of acids without buffering?

    <p>It causes lethal changes in the body fluid H+ concentration</p> Signup and view all the answers

    What is required for the formation of H2CO3 in the body?

    <p>Presence of carbonic anhydrase</p> Signup and view all the answers

    Which buffer system is quantitatively most important in the extracellular fluid?

    <p>Bicarbonate buffer system</p> Signup and view all the answers

    What does ingestion or production of about 80 milliequivalents of H+ each day by metabolism imply?

    <p>The body has efficient buffering mechanisms</p> Signup and view all the answers

    What happens to the H2CO3 formation if carbonic anhydrase is absent?

    <p>No formation of H2CO3 occurs</p> Signup and view all the answers

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