Enzyme Activity and Temperature Effects

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Questions and Answers

In this experiment, what is the independent variable being tested?

Temperature

What is the source of the enzyme catalase in this experiment?

Potato or liver

What is used to measure the rate of reaction in this experiment?

Volume of oxygen gas

Why is it important to use a water bath in this experiment?

To maintain specific temperatures Signup and view all the answers

What is the function of the delivery tube in setting up the reaction?

To collect oxygen gas Signup and view all the answers

What is the controlled variable related to the amount of enzyme?

5g of blended potato or liver Signup and view all the answers

Why is it important to repeat the experiment multiple times for each temperature?

To ensure reliability Signup and view all the answers

According to the hypothesis, what happens to enzyme activity beyond the optimum temperature?

Activity decreases due to denaturation Signup and view all the answers

What is the purpose of using a water bath in this experiment?

To maintain a consistent temperature for the hydrogen peroxide and enzyme solution. (C) Signup and view all the answers

The concentration of hydrogen peroxide is a controlled variable in this experiment.

True (A) Signup and view all the answers

The enzyme catalase breaks down hydrogen peroxide into ______ and water.

oxygen Signup and view all the answers

What is the independent variable being tested in this experiment?

Temperature Signup and view all the answers

Match the following materials with their corresponding functions in the experiment:

Hydrogen peroxide solution = The substrate for the catalase enzyme Potato or liver = Source of catalase enzyme Test tubes = Containers for the reaction mixture Stopwatch = Used to measure the duration of the reaction Graduated cylinder = Measure the volume of oxygen gas produced Signup and view all the answers

Which of the following best describes the dependent variable in this experiment?

The volume of oxygen gas produced (C) Signup and view all the answers

The experiment uses a measuring pipette to add the enzyme solution to the test tube.

True (A) Signup and view all the answers

What type of biological molecule is catalase?

enzyme (or protein) Signup and view all the answers

The purpose of the experiment is to investigate the effect of temperature on the activity of the enzyme ______.

catalase Signup and view all the answers

Match the following laboratory equipment to its function in this experiment:

Test tube = Reaction vessel to mix the hydrogen peroxide and enzyme Stopwatch = Measures the reaction time Delivery tube = Transports oxygen gas to the collection cylinder Water Bath = Maintains specific temperatures Signup and view all the answers

According to the experimental method, how is the enzyme extracted from the biological source?

By filtering the blended mix of potato/liver and water (D) Signup and view all the answers

If the temperature is too high, catalase will work more effectively due to increased kinetic energy.

False (B) Signup and view all the answers

What is the duration allowed for reaction time in each trial?

2 minutes Signup and view all the answers

Study Notes

Experiment Title

Investigating the effect of temperature on enzyme activity (catalase)

Research Question

How does temperature affect the activity of catalase in breaking down hydrogen peroxide?

Hypothesis

If temperature increases up to an optimum level, the rate of reaction will increase.
Beyond the optimum temperature, enzyme activity will decrease due to denaturation.

Variables

Independent Variable: Temperature (e.g., 0°C, 20°C, 40°C, 60°C, 80°C)
Dependent Variable: Rate of reaction, measured by the volume of oxygen gas produced in a set time.
Controlled Variables:
- Concentration of hydrogen peroxide (e.g., 3% solution)
- Volume of hydrogen peroxide (e.g., 10 mL per trial)
- Source and quantity of catalase (e.g., 5g of blended potato or liver)
- Duration of reaction (e.g., 2 minutes per trial)

Materials

Hydrogen peroxide solution (3% concentration)
Fresh potato or liver (as a source of catalase)
Test tubes
Test tube rack
Thermometer
Water baths (to maintain specific temperatures)
Stopwatch
Graduated cylinder
Measuring pipette
Beaker
Delivery tube and gas collection setup (e.g., inverted measuring cylinder in a water trough)

Method

Preparation: Blend potato or liver with water to extract catalase, filtering the mixture to remove large particles.
Temperature Adjustment: Use water baths to adjust the temperature of the hydrogen peroxide solution. Ensure enzyme extract reaches the same temperature by placing it in a water bath.
Reaction Setup: Add 10 mL of hydrogen peroxide to a test tube, followed by 5 mL of blended enzyme solution. Immediately seal the tube. Position the delivery tube within an inverted measuring cylinder filled with water to capture oxygen gas.
Timing: Immediately start the stopwatch upon adding the enzyme solution and measure the volume of oxygen collected after 2 minutes.
Repeat: Conduct the experiment three times for each temperature to ensure reliable results.

Data Collection

Record the volume of oxygen gas produced at each temperature.

Analysis

Create a table with temperature, volume of oxygen produced, and averages for multiple trials.
Plot a graph of temperature (x-axis) vs. volume of oxygen produced (y-axis).
Identify the temperature at which enzyme activity is highest (optimum temperature).
Identify the point where enzyme activity decreases due to denaturation.

Conclusion

Discuss whether the results support the hypothesis.
Explain the observed trends using scientific concepts (e.g., enzyme denaturation at high temperatures).

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Description

This quiz explores the impact of temperature on the activity of catalase as it breaks down hydrogen peroxide. Understand the variables involved, including independent, dependent, and controlled factors, as well as the experimental setup necessary to investigate this relationship. Test your knowledge on enzyme kinetics and the optimum conditions for catalase activity.