Enzyme Activity and Temperature Effects

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Questions and Answers

In this experiment, what is the independent variable being tested?

Temperature

What is the source of the enzyme catalase in this experiment?

Potato or liver

What is used to measure the rate of reaction in this experiment?

Volume of oxygen gas

Why is it important to use a water bath in this experiment?

<p>To maintain specific temperatures</p> Signup and view all the answers

What is the function of the delivery tube in setting up the reaction?

<p>To collect oxygen gas</p> Signup and view all the answers

What is the controlled variable related to the amount of enzyme?

<p>5g of blended potato or liver</p> Signup and view all the answers

Why is it important to repeat the experiment multiple times for each temperature?

<p>To ensure reliability</p> Signup and view all the answers

According to the hypothesis, what happens to enzyme activity beyond the optimum temperature?

<p>Activity decreases due to denaturation</p> Signup and view all the answers

What is the purpose of using a water bath in this experiment?

<p>To maintain a consistent temperature for the hydrogen peroxide and enzyme solution. (C)</p> Signup and view all the answers

The concentration of hydrogen peroxide is a controlled variable in this experiment.

<p>True (A)</p> Signup and view all the answers

The enzyme catalase breaks down hydrogen peroxide into ______ and water.

<p>oxygen</p> Signup and view all the answers

What is the independent variable being tested in this experiment?

<p>Temperature</p> Signup and view all the answers

Match the following materials with their corresponding functions in the experiment:

<p>Hydrogen peroxide solution = The substrate for the catalase enzyme Potato or liver = Source of catalase enzyme Test tubes = Containers for the reaction mixture Stopwatch = Used to measure the duration of the reaction Graduated cylinder = Measure the volume of oxygen gas produced</p> Signup and view all the answers

Which of the following best describes the dependent variable in this experiment?

<p>The volume of oxygen gas produced (C)</p> Signup and view all the answers

The experiment uses a measuring pipette to add the enzyme solution to the test tube.

<p>True (A)</p> Signup and view all the answers

What type of biological molecule is catalase?

<p>enzyme (or protein)</p> Signup and view all the answers

The purpose of the experiment is to investigate the effect of temperature on the activity of the enzyme ______.

<p>catalase</p> Signup and view all the answers

Match the following laboratory equipment to its function in this experiment:

<p>Test tube = Reaction vessel to mix the hydrogen peroxide and enzyme Stopwatch = Measures the reaction time Delivery tube = Transports oxygen gas to the collection cylinder Water Bath = Maintains specific temperatures</p> Signup and view all the answers

According to the experimental method, how is the enzyme extracted from the biological source?

<p>By filtering the blended mix of potato/liver and water (D)</p> Signup and view all the answers

If the temperature is too high, catalase will work more effectively due to increased kinetic energy.

<p>False (B)</p> Signup and view all the answers

What is the duration allowed for reaction time in each trial?

<p>2 minutes</p> Signup and view all the answers

Flashcards

Independent Variable

The variable that is manipulated or changed in an experiment. In this experiment, it is the temperature of the reaction.

Dependent Variable

The variable that is measured or observed in an experiment. In this experiment, it is the rate of oxygen gas production, which is a measure of enzyme activity.

Controlled Variables

Variables that are kept constant throughout an experiment to ensure that any changes observed are due to the independent variable.

Catalase

The enzyme that breaks down hydrogen peroxide into water and oxygen gas. It is found in many organisms, including potatoes and liver.

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Optimum Temperature

The optimal temperature is the temperature at which an enzyme functions best. Beyond this temperature, the enzyme activity decreases due to denaturation.

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Enzyme Denaturation

The process where an enzyme loses its shape and function due to extreme temperatures or other factors. This can happen when the temperature is too high.

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Rate of Reaction

The rate of reaction is the speed at which the reaction occurs. It can be measured by the amount of product formed in a given time.

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Extracting Catalase

The process of extracting the catalase enzyme from the source (potato or liver) by blending it with water and then filtering out any large particles.

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How is enzyme activity measured?

In the context of this experiment, the volume of oxygen produced is the key indicator of how much catalase activity is happening. More oxygen means the enzyme is working harder.

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What happens to catalase enzyme beyond its optimum temperature?

This means that the enzyme is losing its shape and can no longer function properly.

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What is the optimum temperature for catalase activity?

This is the range of temperatures where catalase functions most effectively, leading to the highest rate of reaction.

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What is the independent variable in this experiment?

This means that the temperature is the factor being changed in the experiment to see its effect on catalase activity.

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What is the dependent variable in this experiment?

This means that any changes observed in the oxygen production are directly caused by the changes in temperature.

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What are controlled variables and why are they important?

These are the factors that are kept consistent throughout the experiment, ensuring that any changes in activity are solely due to temperature. These factors include the concentration and volume of hydrogen peroxide, the source and quantity of catalase, and the duration of the reaction.

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What is the reaction catalyzed by catalase?

This is the process of breaking down hydrogen peroxide into water and oxygen gas and is accelerated by the enzyme catalase.

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How is catalase extracted from the source?

The enzyme catalase is extracted from the source by blending it with water. This creates a solution containing the enzyme, which is then filtered to remove any large particles.

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What is catalase?

The enzyme that speeds up the breakdown of hydrogen peroxide into water and oxygen gas, found in many organisms.

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What does a higher rate of reaction mean?

This means the reaction is happening faster, and more oxygen is being produced.

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What is the optimum temperature for catalase?

The temperature at which an enzyme works best, maximizing its activity.

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What happens to catalase when the temperature is too high?

The enzyme loses its shape and can no longer function properly.

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What are controlled variables?

Factors kept constant to ensure any changes in enzyme activity are due to temperature alone.

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How is catalase extracted?

This involves blending the potato or liver with water, creating a solution containing the enzyme, and then filtering out any large particles.

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Study Notes

Experiment Title

  • Investigating the effect of temperature on enzyme activity (catalase)

Research Question

  • How does temperature affect the activity of catalase in breaking down hydrogen peroxide?

Hypothesis

  • If temperature increases up to an optimum level, the rate of reaction will increase.
  • Beyond the optimum temperature, enzyme activity will decrease due to denaturation.

Variables

  • Independent Variable: Temperature (e.g., 0°C, 20°C, 40°C, 60°C, 80°C)
  • Dependent Variable: Rate of reaction, measured by the volume of oxygen gas produced in a set time.
  • Controlled Variables:
    • Concentration of hydrogen peroxide (e.g., 3% solution)
    • Volume of hydrogen peroxide (e.g., 10 mL per trial)
    • Source and quantity of catalase (e.g., 5g of blended potato or liver)
    • Duration of reaction (e.g., 2 minutes per trial)

Materials

  • Hydrogen peroxide solution (3% concentration)
  • Fresh potato or liver (as a source of catalase)
  • Test tubes
  • Test tube rack
  • Thermometer
  • Water baths (to maintain specific temperatures)
  • Stopwatch
  • Graduated cylinder
  • Measuring pipette
  • Beaker
  • Delivery tube and gas collection setup (e.g., inverted measuring cylinder in a water trough)

Method

  • Preparation: Blend potato or liver with water to extract catalase, filtering the mixture to remove large particles.
  • Temperature Adjustment: Use water baths to adjust the temperature of the hydrogen peroxide solution. Ensure enzyme extract reaches the same temperature by placing it in a water bath.
  • Reaction Setup: Add 10 mL of hydrogen peroxide to a test tube, followed by 5 mL of blended enzyme solution. Immediately seal the tube. Position the delivery tube within an inverted measuring cylinder filled with water to capture oxygen gas.
  • Timing: Immediately start the stopwatch upon adding the enzyme solution and measure the volume of oxygen collected after 2 minutes.
  • Repeat: Conduct the experiment three times for each temperature to ensure reliable results.

Data Collection

  • Record the volume of oxygen gas produced at each temperature.

Analysis

  • Create a table with temperature, volume of oxygen produced, and averages for multiple trials.
  • Plot a graph of temperature (x-axis) vs. volume of oxygen produced (y-axis).
  • Identify the temperature at which enzyme activity is highest (optimum temperature).
  • Identify the point where enzyme activity decreases due to denaturation.

Conclusion

  • Discuss whether the results support the hypothesis.
  • Explain the observed trends using scientific concepts (e.g., enzyme denaturation at high temperatures).

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