Podcast
Questions and Answers
In this experiment, what is the independent variable being tested?
In this experiment, what is the independent variable being tested?
Temperature
What is the source of the enzyme catalase in this experiment?
What is the source of the enzyme catalase in this experiment?
Potato or liver
What is used to measure the rate of reaction in this experiment?
What is used to measure the rate of reaction in this experiment?
Volume of oxygen gas
Why is it important to use a water bath in this experiment?
Why is it important to use a water bath in this experiment?
What is the function of the delivery tube in setting up the reaction?
What is the function of the delivery tube in setting up the reaction?
What is the controlled variable related to the amount of enzyme?
What is the controlled variable related to the amount of enzyme?
Why is it important to repeat the experiment multiple times for each temperature?
Why is it important to repeat the experiment multiple times for each temperature?
According to the hypothesis, what happens to enzyme activity beyond the optimum temperature?
According to the hypothesis, what happens to enzyme activity beyond the optimum temperature?
What is the purpose of using a water bath in this experiment?
What is the purpose of using a water bath in this experiment?
The concentration of hydrogen peroxide is a controlled variable in this experiment.
The concentration of hydrogen peroxide is a controlled variable in this experiment.
The enzyme catalase breaks down hydrogen peroxide into ______ and water.
The enzyme catalase breaks down hydrogen peroxide into ______ and water.
What is the independent variable being tested in this experiment?
What is the independent variable being tested in this experiment?
Match the following materials with their corresponding functions in the experiment:
Match the following materials with their corresponding functions in the experiment:
Which of the following best describes the dependent variable in this experiment?
Which of the following best describes the dependent variable in this experiment?
The experiment uses a measuring pipette to add the enzyme solution to the test tube.
The experiment uses a measuring pipette to add the enzyme solution to the test tube.
What type of biological molecule is catalase?
What type of biological molecule is catalase?
The purpose of the experiment is to investigate the effect of temperature on the activity of the enzyme ______.
The purpose of the experiment is to investigate the effect of temperature on the activity of the enzyme ______.
Match the following laboratory equipment to its function in this experiment:
Match the following laboratory equipment to its function in this experiment:
According to the experimental method, how is the enzyme extracted from the biological source?
According to the experimental method, how is the enzyme extracted from the biological source?
If the temperature is too high, catalase will work more effectively due to increased kinetic energy.
If the temperature is too high, catalase will work more effectively due to increased kinetic energy.
What is the duration allowed for reaction time in each trial?
What is the duration allowed for reaction time in each trial?
Flashcards
Independent Variable
Independent Variable
The variable that is manipulated or changed in an experiment. In this experiment, it is the temperature of the reaction.
Dependent Variable
Dependent Variable
The variable that is measured or observed in an experiment. In this experiment, it is the rate of oxygen gas production, which is a measure of enzyme activity.
Controlled Variables
Controlled Variables
Variables that are kept constant throughout an experiment to ensure that any changes observed are due to the independent variable.
Catalase
Catalase
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Optimum Temperature
Optimum Temperature
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Enzyme Denaturation
Enzyme Denaturation
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Rate of Reaction
Rate of Reaction
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Extracting Catalase
Extracting Catalase
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How is enzyme activity measured?
How is enzyme activity measured?
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What happens to catalase enzyme beyond its optimum temperature?
What happens to catalase enzyme beyond its optimum temperature?
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What is the optimum temperature for catalase activity?
What is the optimum temperature for catalase activity?
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What is the independent variable in this experiment?
What is the independent variable in this experiment?
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What is the dependent variable in this experiment?
What is the dependent variable in this experiment?
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What are controlled variables and why are they important?
What are controlled variables and why are they important?
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What is the reaction catalyzed by catalase?
What is the reaction catalyzed by catalase?
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How is catalase extracted from the source?
How is catalase extracted from the source?
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What is catalase?
What is catalase?
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What does a higher rate of reaction mean?
What does a higher rate of reaction mean?
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What is the optimum temperature for catalase?
What is the optimum temperature for catalase?
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What happens to catalase when the temperature is too high?
What happens to catalase when the temperature is too high?
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What are controlled variables?
What are controlled variables?
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How is catalase extracted?
How is catalase extracted?
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Study Notes
Experiment Title
- Investigating the effect of temperature on enzyme activity (catalase)
Research Question
- How does temperature affect the activity of catalase in breaking down hydrogen peroxide?
Hypothesis
- If temperature increases up to an optimum level, the rate of reaction will increase.
- Beyond the optimum temperature, enzyme activity will decrease due to denaturation.
Variables
- Independent Variable: Temperature (e.g., 0°C, 20°C, 40°C, 60°C, 80°C)
- Dependent Variable: Rate of reaction, measured by the volume of oxygen gas produced in a set time.
- Controlled Variables:
- Concentration of hydrogen peroxide (e.g., 3% solution)
- Volume of hydrogen peroxide (e.g., 10 mL per trial)
- Source and quantity of catalase (e.g., 5g of blended potato or liver)
- Duration of reaction (e.g., 2 minutes per trial)
Materials
- Hydrogen peroxide solution (3% concentration)
- Fresh potato or liver (as a source of catalase)
- Test tubes
- Test tube rack
- Thermometer
- Water baths (to maintain specific temperatures)
- Stopwatch
- Graduated cylinder
- Measuring pipette
- Beaker
- Delivery tube and gas collection setup (e.g., inverted measuring cylinder in a water trough)
Method
- Preparation: Blend potato or liver with water to extract catalase, filtering the mixture to remove large particles.
- Temperature Adjustment: Use water baths to adjust the temperature of the hydrogen peroxide solution. Ensure enzyme extract reaches the same temperature by placing it in a water bath.
- Reaction Setup: Add 10 mL of hydrogen peroxide to a test tube, followed by 5 mL of blended enzyme solution. Immediately seal the tube. Position the delivery tube within an inverted measuring cylinder filled with water to capture oxygen gas.
- Timing: Immediately start the stopwatch upon adding the enzyme solution and measure the volume of oxygen collected after 2 minutes.
- Repeat: Conduct the experiment three times for each temperature to ensure reliable results.
Data Collection
- Record the volume of oxygen gas produced at each temperature.
Analysis
- Create a table with temperature, volume of oxygen produced, and averages for multiple trials.
- Plot a graph of temperature (x-axis) vs. volume of oxygen produced (y-axis).
- Identify the temperature at which enzyme activity is highest (optimum temperature).
- Identify the point where enzyme activity decreases due to denaturation.
Conclusion
- Discuss whether the results support the hypothesis.
- Explain the observed trends using scientific concepts (e.g., enzyme denaturation at high temperatures).
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