Enzyme Activity and Temperature

What happens to the enzyme's active site when the temperature exceeds the optimum temperature?

Which of the following temperatures was not included in the experimental setup for measuring catalase activity?

Why is temperature considered a key factor in enzyme activity?

What was the purpose of using detergent in the experimental setup?

What conclusion can be drawn from the comparison of the experiment's data with the original data sets regarding temperature?

Enzyme Activity and Temperature

Aim: To investigate the effect of temperature on enzyme activity, specifically looking at catalase.
Enzymes are biological catalysts speeding up chemical reactions in living cells, remaining unchanged during the process.
Enzymes are made from proteins, which are composed of amino acids.
Temperature is a key factor affecting enzyme activity.
Optimum temperature: The temperature at which enzyme activity is highest.
Beyond the optimum temperature, the active site of the enzyme changes shape, preventing substrate binding and reducing activity.
Degradation reactions break down larger molecules into smaller ones. Hydrogen peroxide is a substrate that catalase breaks down into water and oxygen.

Experiment Setup and Procedure

Setup: Multiple boiling tubes with hydrogen peroxide, the enzyme catalase, and different temperatures.
Procedure: Add the solutions and tubes to different temperatures, allowing them to react for 5 minutes.
Measurement: Measure the height of the oxygen foam produced in each reaction tube after the 5-minute period.
Source differences: The recorded temperature data may differ from the source data, potentially due to temperature disparities (4°C, 19°C, 37°C, 50°C used compared to possible data recording at differing temperatures).

This quiz explores the relationship between temperature and the activity of enzymes, specifically catalase. Discover how temperature influences enzyme behavior, the concept of optimum temperature, and the effects of degradation reactions. Test your understanding of these biological catalysts in various experimental setups.