Podcast
Questions and Answers
What happens to the enzyme's active site when the temperature exceeds the optimum temperature?
What happens to the enzyme's active site when the temperature exceeds the optimum temperature?
- The active site becomes more flexible.
- The active site changes shape, preventing substrate binding. (correct)
- The active site increases its affinity for the substrate.
- The enzyme becomes more effective at catalyzing reactions.
Which of the following temperatures was not included in the experimental setup for measuring catalase activity?
Which of the following temperatures was not included in the experimental setup for measuring catalase activity?
- 50°C
- 48°C (correct)
- 19°C
- 4°C
Why is temperature considered a key factor in enzyme activity?
Why is temperature considered a key factor in enzyme activity?
- Higher temperatures always increase enzyme production.
- Lower temperatures enhance the substrate concentration in reactions.
- Temperature directly affects the pH of the solution where the enzyme operates.
- Temperature influences the shape and function of the enzyme's active site. (correct)
What was the purpose of using detergent in the experimental setup?
What was the purpose of using detergent in the experimental setup?
What conclusion can be drawn from the comparison of the experiment's data with the original data sets regarding temperature?
What conclusion can be drawn from the comparison of the experiment's data with the original data sets regarding temperature?
Flashcards
Optimum Temperature
Optimum Temperature
The temperature at which an enzyme functions most effectively, leading to the highest rate of reaction.
Enzymes
Enzymes
Biological catalysts made of proteins that accelerate chemical reactions in living organisms.
Active Site
Active Site
The specific region on an enzyme where the substrate binds and interacts.
Substrate
Substrate
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Denaturation
Denaturation
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Study Notes
Enzyme Activity and Temperature
- Aim: To investigate the effect of temperature on enzyme activity, specifically looking at catalase.
- Enzymes are biological catalysts speeding up chemical reactions in living cells, remaining unchanged during the process.
- Enzymes are made from proteins, which are composed of amino acids.
- Temperature is a key factor affecting enzyme activity.
- Optimum temperature: The temperature at which enzyme activity is highest.
- Beyond the optimum temperature, the active site of the enzyme changes shape, preventing substrate binding and reducing activity.
- Degradation reactions break down larger molecules into smaller ones. Hydrogen peroxide is a substrate that catalase breaks down into water and oxygen.
Experiment Setup and Procedure
- Setup: Multiple boiling tubes with hydrogen peroxide, the enzyme catalase, and different temperatures.
- Procedure: Add the solutions and tubes to different temperatures, allowing them to react for 5 minutes.
- Measurement: Measure the height of the oxygen foam produced in each reaction tube after the 5-minute period.
- Source differences: The recorded temperature data may differ from the source data, potentially due to temperature disparities (4°C, 19°C, 37°C, 50°C used compared to possible data recording at differing temperatures).
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