11
29 Questions
0 Views

Choose a study mode

Play Quiz
Study Flashcards
Spaced Repetition
Chat to lesson

Podcast

Play an AI-generated podcast conversation about this lesson

Questions and Answers

What is the purpose of enrichment cultures in microbiology?

  • To quantify the number of microorganisms in a sample
  • To identify the relative abundance of microbial communities
  • To study the ecological importance of microorganisms
  • To isolate bacteria from diverse and densely populated samples (correct)

    • What does enrichment bias in laboratory cultures indicate?

  • Microorganisms cultured in the lab are often minor components of the microbial ecosystem (correct)
  • The lab environment provides more favorable conditions for microbial growth than natural habitats
  • The microbial ecosystem in the lab is entirely different from natural habitats
  • Microorganisms cultured in the lab are always the dominant species in the environment

    • What is the most probable number technique commonly used for?

  • Quantifying the relative abundance of microbial communities
  • Estimating the number of microorganisms in food, wastewater, and other samples (correct)
  • Identifying the ecological importance of microorganisms
  • Isolating pure cultures of microorganisms

    • What does a successful enrichment culture indicate?

    <p>It has appropriate resources and conditions needed for target organisms to grow</p> Signup and view all the answers

    What is the purpose of dilution of inoculum in classical procedures for isolating microorganisms?

    <p>To eliminate rapidly growing, but quantitatively insignificant species</p> Signup and view all the answers

    What does enrichment culture not provide information about?

    <p>Relative abundance and ecological importance of the target organism</p> Signup and view all the answers

    What is the purpose of enrichment cultures in microbiology?

    <p>To isolate bacteria from diverse and densely populated samples</p> Signup and view all the answers

    What is the purpose of dilution of inoculum in classical procedures for isolating microorganisms?

    <p>To eliminate rapidly growing, but quantitatively insignificant species</p> Signup and view all the answers

    What is the main purpose of stable isotope probing?

    <p>To identify microorganisms that can utilize specific substrates in a microbial community</p> Signup and view all the answers

    What is the primary focus of metatranscriptomics?

    <p>Analysis of community mRNA to reveal active genes</p> Signup and view all the answers

    What is the main purpose of environmental genomics (metagenomics)?

    <p>To identify as many genes as possible from DNA extracted from environmental samples</p> Signup and view all the answers

    What is the primary focus of metaproteomics?

    <p>Measuring the diversity and abundance of different proteins in a community</p> Signup and view all the answers

    What is the purpose of isotopic fractionation in biological reactions?

    <p>Biological reactions prefer lighter isotopes, leading to isotopic fractionation</p> Signup and view all the answers

    What is the main advantage of DNA microarray containing gene probes?

    <p>It provides functional information to correlate with phylogenetic analysis</p> Signup and view all the answers

    What is the primary purpose of environmental multi-omics?

    <p>To provide an integrated accounting of all central cellular processes</p> Signup and view all the answers

    What is the main focus of direct chemical measurements of metabolites?

    <p>For many studies, direct chemical measurements are sufficient for measuring specific metabolites</p> Signup and view all the answers

    What is the primary function of microsensors?

    <p>To measure a wide range of activities such as pH and oxygen</p> Signup and view all the answers

    What is the purpose of laser tweezers in microbiology?

    <p>Isolating single cells from mixed cultures</p> Signup and view all the answers

    What is the main function of flow cytometry in microbiology?

    <p>Counting and examining mixtures of cells</p> Signup and view all the answers

    What is the primary purpose of high-throughput cultivation of previously uncultured microorganisms?

    <p>Separating individual cells for culture in microtitre plate, one cell per well</p> Signup and view all the answers

    What is the general principle of nucleic acid hybridization in microbial ecology?

    <p>Nucleic acids with complementary sequence can form hybrid</p> Signup and view all the answers

    What is the main purpose of fluorescence in situ hybridization (FISH) in microbiology?

    <p>Used to see microorganisms in the context of their communities</p> Signup and view all the answers

    What is the primary focus of PCR methods of microbial community analysis?

    <p>Amplification of specific genes for diversity analysis</p> Signup and view all the answers

    What is the purpose of massively parallel DNA sequencing in phylogenetic analysis?

    <p>Generate billions of sequence reads for detecting minor phylotypes</p> Signup and view all the answers

    What is the main function of geochip in microbiology?

    <p>Functional gene microarray to approximate gene abundance</p> Signup and view all the answers

    What is the purpose of viability stains in microbiology?

    <p>Providing information on abundance and viability of cells</p> Signup and view all the answers

    What is the main focus of general staining to examine microbial communities?

    <p>Culture-independent microscopic method of examining microbial communities</p> Signup and view all the answers

    What is the primary purpose of microfluidic platform for cultivation in microbiology?

    <p>Constructing tiny wells for cultivation, one cell per well</p> Signup and view all the answers

    What is the main function of fluorescent protein reporters in microbiology?

    <p>Tracking live bacteria and bacterial processes</p> Signup and view all the answers

    More Like This

    Use Quizgecko on...
    Browser
    Open
    Browser
    Browser