12 Questions
What type of data are scientific digital images?
Numerical data arranged spatially
According to Dr. John Russ, what is a key point regarding human observers and image interpretation?
Humans often see what they expect to see
Why should manipulation of digital images be done on a copy of the unprocessed image data file?
To maintain the standard for comparison with the final image
What do the individual elements in a digital image represent?
Grayscale or RGB intensity values
What is the responsibility of the user regarding understanding instrument limitations?
To comprehend the constraints of the particular instrument used
How should the final image be compared after manipulation according to the text?
Use it as the new standard reference
According to the MSA Policy on Digital Image Manipulation, which of the following operations is generally considered acceptable without needing to be reported?
Histogram stretching
What is required for any imaging operations other than gamma correction, histogram stretching, and brightness/contrast adjustments?
They must be reported as part of the experimental methodology.
What is the primary requirement for the original uncompressed image file, according to the MSA Policy?
It must be stored on archival media (e.g., CD-R) without any manipulation.
For diffraction data or any other image data used for subsequent quantification, what is required according to the MSA Policy?
All imaging operations must be reported.
What is the primary purpose of the MSA Policy on Digital Image Manipulation?
To ensure reproducibility of image processing operations.
According to the guidelines provided, which of the following statements is true?
The guidelines were written for life science imaging but are also relevant to materials science microscopy.
Study Notes
Ethical Digital Imaging Policy
- The MSA policy on Digital Image Manipulation advises EMS users to store original, uncompressed image files on archival media (e.g., CD-R) without any image manipulation or processing operations.
- All parameters of image production and acquisition, as well as subsequent processing steps, must be documented and reported to ensure reproducibility.
Acceptable Image Operations
- Acceptable, non-reportable imaging operations include gamma correction, histogram stretching, and brightness and contrast adjustments.
- Other operations (e.g., Unsharp-Masking, Gaussian Blur) must be directly identified by the author as part of the experimental methodology.
Guidelines for Scientific Digital Image Acquisition and Manipulation
- Scientific digital images are data that can be compromised by inappropriate manipulations.
- Images are data arranged spatially in an XY matrix (or grid) and each individual element (pixel) has a numerical value that represents a grayscale or RGB intensity value.
- The data acquisition system and sensor are subject to limitations and aberrations imposed by physics and instrument design.
- The user is responsible for understanding the limitations of the particular instrument.
Best Practices
- Manipulation of digital images should always be done on a copy of the unprocessed image data file.
- The original raw data file is the standard to which the final image can and should be compared.
This quiz focuses on the importance of following the MSA Policy on Digital Image Manipulation for EMS Users. It covers key guidelines such as storing original image files on archival media, documenting parameters of production and acquisition, and ensuring reproducibility of processing steps.
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