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EMS Users and MSA Policy on Digital Image Manipulation

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EMS Users and MSA Policy on Digital Image Manipulation

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Questions and Answers

What type of data are scientific digital images?

Temporal data representing time intervals

Qualitative data with no numerical values

Abstract data without any structure

Numerical data arranged spatially (correct)

According to Dr. John Russ, what is a key point regarding human observers and image interpretation?

Humans are excellent observers with minimal limitations

Humans often see what they expect to see (correct)

Having names and labels for recognized features is not important

Our vision system captures all available information accurately

Why should manipulation of digital images be done on a copy of the unprocessed image data file?

To enhance the original raw data file

To eliminate the need for understanding instrument limitations

To avoid any limitations imposed by physics and instrument design

To maintain the standard for comparison with the final image (correct)

What do the individual elements in a digital image represent?

Grayscale or RGB intensity values Signup and view all the answers

What is the responsibility of the user regarding understanding instrument limitations?

To comprehend the constraints of the particular instrument used Signup and view all the answers

How should the final image be compared after manipulation according to the text?

Use it as the new standard reference Signup and view all the answers

According to the MSA Policy on Digital Image Manipulation, which of the following operations is generally considered acceptable without needing to be reported?

Histogram stretching Signup and view all the answers

What is required for any imaging operations other than gamma correction, histogram stretching, and brightness/contrast adjustments?

They must be reported as part of the experimental methodology. Signup and view all the answers

What is the primary requirement for the original uncompressed image file, according to the MSA Policy?

It must be stored on archival media (e.g., CD-R) without any manipulation. Signup and view all the answers

For diffraction data or any other image data used for subsequent quantification, what is required according to the MSA Policy?

All imaging operations must be reported. Signup and view all the answers

What is the primary purpose of the MSA Policy on Digital Image Manipulation?

To ensure reproducibility of image processing operations. Signup and view all the answers

According to the guidelines provided, which of the following statements is true?

The guidelines were written for life science imaging but are also relevant to materials science microscopy. Signup and view all the answers

Study Notes

Ethical Digital Imaging Policy

The MSA policy on Digital Image Manipulation advises EMS users to store original, uncompressed image files on archival media (e.g., CD-R) without any image manipulation or processing operations.
All parameters of image production and acquisition, as well as subsequent processing steps, must be documented and reported to ensure reproducibility.

Acceptable Image Operations

Acceptable, non-reportable imaging operations include gamma correction, histogram stretching, and brightness and contrast adjustments.
Other operations (e.g., Unsharp-Masking, Gaussian Blur) must be directly identified by the author as part of the experimental methodology.

Guidelines for Scientific Digital Image Acquisition and Manipulation

Scientific digital images are data that can be compromised by inappropriate manipulations.
Images are data arranged spatially in an XY matrix (or grid) and each individual element (pixel) has a numerical value that represents a grayscale or RGB intensity value.
The data acquisition system and sensor are subject to limitations and aberrations imposed by physics and instrument design.
The user is responsible for understanding the limitations of the particular instrument.

Best Practices

Manipulation of digital images should always be done on a copy of the unprocessed image data file.
The original raw data file is the standard to which the final image can and should be compared.

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Description

This quiz focuses on the importance of following the MSA Policy on Digital Image Manipulation for EMS Users. It covers key guidelines such as storing original image files on archival media, documenting parameters of production and acquisition, and ensuring reproducibility of processing steps.