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Questions and Answers
What is the name of the outer ring of cells in a blastocyst?
What is the name of the outer ring of cells in a blastocyst?
What is the primary source of embryonic stem (ES) cells?
What is the primary source of embryonic stem (ES) cells?
What is the name of the process by which one cell population influences the development of neighboring cells?
What is the name of the process by which one cell population influences the development of neighboring cells?
What are cytoplasmic determinants?
What are cytoplasmic determinants?
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Which of the following is NOT a feature of the morula stage of development?
Which of the following is NOT a feature of the morula stage of development?
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What is the main purpose of cellular differentiation?
What is the main purpose of cellular differentiation?
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Which of the following plays a crucial role in mediating proliferation, differentiation, and apoptosis during development?
Which of the following plays a crucial role in mediating proliferation, differentiation, and apoptosis during development?
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What happens to the zygote after fertilization in the oviduct?
What happens to the zygote after fertilization in the oviduct?
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What is the first stage of embryonic development following fertilization?
What is the first stage of embryonic development following fertilization?
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Which of the following factors is NOT mentioned as determining haemopoiesis and muscle cell differentiation?
Which of the following factors is NOT mentioned as determining haemopoiesis and muscle cell differentiation?
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Which retinoid is classified as a pan-RAR agonist?
Which retinoid is classified as a pan-RAR agonist?
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Which of the following best describes the primary target of bexarotene?
Which of the following best describes the primary target of bexarotene?
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What is the trade name for all-trans retinoic acid (ATRA)?
What is the trade name for all-trans retinoic acid (ATRA)?
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What is an irreversible characteristic associated with mature keratinocytes?
What is an irreversible characteristic associated with mature keratinocytes?
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Which of the following represents a reversible characteristic in cells?
Which of the following represents a reversible characteristic in cells?
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Acyclic retinoid exhibits activity against which of the following receptor subtypes?
Acyclic retinoid exhibits activity against which of the following receptor subtypes?
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Which of the following retinoids is used in the treatment of Kaposi's sarcoma?
Which of the following retinoids is used in the treatment of Kaposi's sarcoma?
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What is an example of a marker for terminal differentiation in erythrocytes?
What is an example of a marker for terminal differentiation in erythrocytes?
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What process is primarily responsible for initiating and promoting differentiation in cells?
What process is primarily responsible for initiating and promoting differentiation in cells?
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Considering the provided data, which retinoid is used in combination with interferon for the treatment of some head and neck cancers?
Considering the provided data, which retinoid is used in combination with interferon for the treatment of some head and neck cancers?
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What is a consequence of poorly differentiated tumors in the context of cancer progression?
What is a consequence of poorly differentiated tumors in the context of cancer progression?
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Which of the following is a therapeutic application of Fenretidine?
Which of the following is a therapeutic application of Fenretidine?
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Which retinoid is associated with the trade name 'Panretin'?
Which retinoid is associated with the trade name 'Panretin'?
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What is the role of mesoderm in embryonic development?
What is the role of mesoderm in embryonic development?
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What type of inductive event results in a choice between one fate or another?
What type of inductive event results in a choice between one fate or another?
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Which mechanism allows multiple cell fates based on the level of a signaling molecule?
Which mechanism allows multiple cell fates based on the level of a signaling molecule?
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How is cell type specificity determined during development?
How is cell type specificity determined during development?
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Which of the following best describes the term 'morphogens'?
Which of the following best describes the term 'morphogens'?
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What happens to genes in a given cell during selective gene transcription?
What happens to genes in a given cell during selective gene transcription?
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What characterizes the relay aspect of inductive events?
What characterizes the relay aspect of inductive events?
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What is the significance of transcription in embryonic cell differentiation?
What is the significance of transcription in embryonic cell differentiation?
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What is the primary role of micro-RNAs in cellular processes?
What is the primary role of micro-RNAs in cellular processes?
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Which of the following are components of pancreatic acinar cells?
Which of the following are components of pancreatic acinar cells?
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What role do extracellular factors play in cell differentiation?
What role do extracellular factors play in cell differentiation?
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What is hematopoiesis?
What is hematopoiesis?
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Which of the following correctly describes the function of Dicer in the context of micro-RNAs?
Which of the following correctly describes the function of Dicer in the context of micro-RNAs?
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What are universal blood stem cells capable of forming?
What are universal blood stem cells capable of forming?
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What is a significant effect of binding miRNA to its target mRNA?
What is a significant effect of binding miRNA to its target mRNA?
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Actin and myosin are primarily associated with which type of cells?
Actin and myosin are primarily associated with which type of cells?
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Flashcards
Cell Differentiation
Cell Differentiation
The process by which cells become specialized to perform specific functions, differing from their original state.
Development from Zygote to Adult
Development from Zygote to Adult
Cell differentiation, morphogenesis (shape changes), and cell division all contribute to the development of a complete organism from a single cell.
Cell Interactions and Signaling
Cell Interactions and Signaling
Specialized signaling pathways within cells are turned on or off based on interactions with other cells and the environment, impacting cell division, differentiation, and death.
Stem Cell Differentiation
Stem Cell Differentiation
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Hematopoiesis
Hematopoiesis
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Morula
Morula
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Blastocyst
Blastocyst
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Embryonic Stem Cells
Embryonic Stem Cells
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Cytoplasmic Determinants
Cytoplasmic Determinants
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Induction
Induction
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Induction during development
Induction during development
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Binary induction
Binary induction
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Gradient induction
Gradient induction
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Relay induction
Relay induction
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Morphogen
Morphogen
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Inducer
Inducer
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Cell type specificity
Cell type specificity
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Irreversible Characteristic
Irreversible Characteristic
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Reversible Characteristic
Reversible Characteristic
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Differentiation Process
Differentiation Process
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Markers of Differentiation
Markers of Differentiation
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Heterotypic Cell Interaction
Heterotypic Cell Interaction
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MicroRNA (miRNA)
MicroRNA (miRNA)
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Dicer
Dicer
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RISC (RNA-induced silencing complex)
RISC (RNA-induced silencing complex)
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Hemangioblast
Hemangioblast
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Universal Blood Stem Cells
Universal Blood Stem Cells
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Morphogenesis
Morphogenesis
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Nuclear Receptors
Nuclear Receptors
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Retinoids
Retinoids
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Retinoic Acid Receptor (RAR)
Retinoic Acid Receptor (RAR)
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Retinoid X Receptor (RXR)
Retinoid X Receptor (RXR)
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All-trans Retinoic Acid (ATRA)
All-trans Retinoic Acid (ATRA)
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Retinoids for Chemoprevention
Retinoids for Chemoprevention
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Retinoids for Cancer Therapies
Retinoids for Cancer Therapies
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Retinoid-based Therapy for Promyelocytic Leukemia
Retinoid-based Therapy for Promyelocytic Leukemia
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Study Notes
Cell Differentiation Control
- Cellular differentiation is the process by which cells or parts of an organism become different from each other and their previous state.
- It's also the process by which cells or tissues of an organism acquire the ability to perform specialized functions.
- The transformation from zygote to adult results from cell division, cell differentiation, and morphogenesis.
- Cells interact with each other and their environment. This interaction turns on or off specific signaling pathways.
- These pathways are crucial for mediating proliferation, differentiation, and apoptosis during development.
- Fertilization of an egg takes place in the oviduct.
- The fertilized zygote travels to the uterus for implantation, dividing mitotically (2-cell, 4-cell, 8-cell stages...).
- The embryo reaches the morula stage (a ball of small cells) and the blastocyst stage (the second embryonic stage).
- The ICM cells form embryonic stem cells (ES cells).
Cytoplasmic Determinants and Inductive Signals
- An egg's cytoplasm contains RNA, proteins, and other substances distributed unevenly.
- Cytoplasmic determinants are maternal substances that influence early development.
- As the zygote divides by mitosis, cells contain different cytoplasmic determinants, leading to variations in gene expression.
Cellular Interactions in Development: Induction
- Interactions between germ layer cells influence stem cell fate and differentiation pathways.
- Induction is a mechanism where one cell population influences the development of neighboring cells.
- Example: Mesoderm induces ectoderm to form neural tissue (e.g.).
- Binary induction: a choice between one fate or another based on signal presence or absence.
- Gradient induction: multiple fates depend on signal level (morphogens).
- Relay induction: cascading signals determine cell fates in proximity. These cells produce more signals that affect nearby cells.
Cellular Transduction Pathway
- A sequence of molecular events that transmits a signal from the cell's exterior to the interior to initiate a specific internal response.
- Signal transduction pathways are vital for cell signaling processes, such as growth, cell division, differentiation, and survival.
- Signaling molecules are part of signal transduction.
- These influence gene expressions inside a cell, and ultimately the cell function.
Cell Type Specificity
- Cell uniqueness comes from unique portions of the genome that are expressed.
- The process involves transcription, mRNA production, protein expression, and the execution of a cell's unique function.
Where does it begin differentiation?
- Differentiation begins in the in-vivo (in living organism) fertilized egg, leading to the 8-cell embryo.
- The next stages are totipotent, during cell culture, and pluripotent (blastocyst), which can form various parts of the embryo.
- From these stem cells, further differentiation forms neural cells, muscle cells, and blood cells, and other tissues.
Selective Gene Transcription
- In any cell, only the genes needed for basic metabolism and the cell's specific functions are active.
- Muscle cells express genes for actin and myosin proteins.
- Pancreatic acinar cells produce digestive enzymes.
- Neurons have genes for tubulin and neurotransmitters.
Control of Cell Differentiation (General and Genomic Level)
- Control mechanisms include genomic level control (DNA level), transcription (copying genes into RNA), transcription levels (amount of a gene produced as RNA), and extracellular factors (such as growth factors and hormones).
- Specific factors, like microRNAs, work to regulate specific gene expressions.
Control of Cell Differentiation (MicroRNAs)
- MicroRNAs (miRNAs) control gene expression by affecting gene translation or degradation.
- The process frequently involves base pairing between miRNAs and mRNA sequences to affect transcript expression.
Differentiation of Blood Cells
- Hematopoiesis is the creation of blood cells.
- Universal blood stem cells (produced via the hemangioblast) create myeloid and lymphoid precursors.
- Myeloid precursors create cells like red blood cells (transporting oxygen, carbon dioxide), platelets (blood clotting), monocytes, and granulocytes.
- Lymphoid precursors form lymphocytes (part of the immune system).
Regulation of Hematopoiesis
- Hematopoiesis is regulated by cytokines, growth factors like CSF-1, GATA-1, GATA-2, and others.
- These factors help maintain pluripotent stem cells and their progeny to produce the various blood cell types.
Genetic Control of Muscle Cell Differentiation
- Myoblasts, precursors to muscle cells produce muscle-specific proteins to form skeletal muscle via the myoD protein.
- MyoD is a transcription factor and directs muscle cell development.
- This master regulatory gene activates genes necessary for muscle function.
- Other factors in the myogenic family have similar effects.
Terminal Differentiation
- Cells reach a point where they cannot progress further down a lineage.
- Examples include neurons, skeletal muscle cells, and keratinocytes.
- Fibrocytes are often excluded, as they remain mostly capable of further development in response to signals and surroundings.
Cell Properties
- Irreversible differentiation (e.g., mature keratinocytes): ceasing DNA synthesis.
- Reversible differentiation (e.g., hepatocytes): having the potential to resume producing specific proteins after stimulation or conditions changed.
Markers of Differentiation
- Markers indicate the mature phenotype of a terminal differentiated cell.
- Examples include cytokeratins (epithelium), glial fibrillary acidic protein (astrocytes), hemoglobin (red blood cells), albumin (liver cells).
- These markers or combinations thereof make it possible to identify cell type via the expression of proteins and genes.
Regulation of Differentiation
- Several factors influence differentiation:
- Cell-cell interactions (e.g., homotypic (with similar cells), heterotypic (with different cells), via contact, diffusible factors, including extracellular matrix).
- Extracellular factors (e.g., hormones, growth factors).
- In-vitro factors (e.g., growth-promoting or growth-restricting substances).
- oxygen tension, cellular shape, and cellular matrix
- These interactions lead to signaling that ultimately determines the fate of the cell.
Induction of Differentiation
- Heterotypic interactions cause cellular differentiation by the mutual interaction between cells from different germ layers.
- Reciprocal paracrine signaling triggers specific cell differentiation like epidermal maturation.
- Growth factors (e.g., GM-CSF, KGF) and cytokines (e.g., IL-1α, IL-1β) from various cells in the tissue communicate with other cells, such as the keratinocyte and fibroblast, and have an effect.
Diseases Associated with Differentiation
- Pulmonary fibrosis (e.g., abnormal myofibroblast, fibroblast action leading to collagen build up).
- Cancer (reduced or abnormal differentiation).
Differentiation & Diseases
- Cancer is a failure of cells to differentiate properly.
- Poor differentiation in tumors correlates with a lower survival rate for patients.
- Tumor differentiation can be altered under different conditions and circumstances.
Differentiation & Malignancy
- Cancer is linked to impaired differentiation.
- Histology of a cancer suggests its level of differentiation.
- Patients with poorly differentiated tumors have a lower survival rate.
- Cancer cells in cell culture can be induced to differentiate.
Chemicals Inducing Differentiation
- Certain compounds can trigger the differentiation process in transformed cells.
- Polar and nonpolar substances have been recognized for their potential role and actions.
Clinical Use of Retinoids and Nuclear Receptors
- Retinoids, such as ATRA, are used in cancer therapies.
- The clinical use of retinoids is linked to their effect on nuclear receptors, like RAR and RXR, involved in regulating gene expression.
- Multiple retinoid and receptor combinations exist and are used in medicinal applications.
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Description
Test your knowledge on embryology with this quiz focusing on the blastocyst, cellular differentiation, and the stages of embryonic development. Key concepts like stem cells, cytoplasmic determinants, and retinoids will also be covered. See how well you understand the processes that shape early life.