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Questions and Answers
What is the effect of osmotic potential on the success of embryo rescue?
What is the effect of osmotic potential on the success of embryo rescue?
- It has no effect
- It depends on the type of embryo
- It decreases the success rate
- It increases the success rate (correct)
What is the temperature range for incubation during embryo rescue?
What is the temperature range for incubation during embryo rescue?
- 15-20°C
- 20-25°C
- 25-30°C (correct)
- 10-15°C
Which of the following is NOT a use of embryo rescue?
Which of the following is NOT a use of embryo rescue?
- Creating genetically modified organisms (correct)
- Overcoming seed dormancy
- Studying seed development and germination
- Producing triploids from diploid-tetraploid crosses
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Study Notes
- Embryo rescue involves culturing embryos to obtain viable progeny from difficult hybridizations
- Embryos can be cultured directly or within the ovule to prevent damage during excision
- Embryo rescue can overcome seed dormancy and study seed development and germination
- Media, growth regulators, and osmotic potential affect the success of embryo rescue
- Young embryos require complex media and high osmotic potential to prevent premature germination
- Older embryos have a higher chance of successful rescue due to their ability to synthesize growth substances
- Temperature and light requirements vary among species, with incubation typically at 25-30°C
- Cold treatment may be required for species with seed dormancy
- Embryo rescue is commonly used in interspecific and intergeneric hybridizations
- It is also used in breeding for seedless grapes and to produce triploids from diploid-tetraploid crosses.
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