Embalming Chemistry: Formaldehyde

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Questions and Answers

When was formaldehyde first thought discovered, and when was it formally identified?

Formaldehyde was thought to be discovered in 1859 and was formally identified in 1869.

Formaldehyde is a colorless toxic gas with a disagreeable odor. When dissolved in water, what happens to the vapor?

It becomes trapped in the water.

Formaldehyde is known to be quite reactive and its propensity to polymerize into _____ was quite interesting.

paraformaldehyde

Formaldehyde is commercially sold as what percentage solution in water, typically stabilized by methanol?

<p>37%</p>
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Aqueous solutions of formaldehyde are commonly referred to as formalin. What chemical compound are they primarily composed of in solution, rather than pure formaldehyde?

<p>Methylene glycol</p>
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Who is thought to have first discovered formaldehyde in Russia in 1859?

<p>Alexander Butlerov</p>
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Who formally identified formaldehyde in 1869 at the University of Berlin?

<p>Professor August Hoffman</p>
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In its basic, pure form, what state of matter is formaldehyde?

<p>Gas</p>
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What liquid mixture consists of formaldehyde gas dissolved in water?

<p>Formalin</p>
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What percentage range of methyl alcohol (methanol) is typically added to formaldehyde solutions to prevent polymerization?

<p>10-15%</p>
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The addition of which substance typically causes an aqueous formaldehyde solution to be specifically termed 'formalin'?

<p>Methyl alcohol (methanol)</p>
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What is the solid form of formaldehyde called?

<p>Paraformaldehyde</p>
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Due to the potential hazards associated with formaldehyde use, what does OSHA specifically require employers to perform?

<p>Air Sampling</p>
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What is the Permissible Exposure Limit (PEL) set by OSHA for formaldehyde, expressed as an 8-hour time-weighted average (TWA)?

<p>0.75 ppm</p>
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What is the Short-Term Exposure Level (STEL) set by OSHA for formaldehyde, measured over a maximum 15-minute period?

<p>2 ppm</p>
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What is the Action Level set by OSHA for formaldehyde, expressed as an 8-hour time-weighted average (TWA)?

<p>0.5 ppm</p>
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In what year was the OSHA Formaldehyde Standard passed?

<p>1992</p>
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What time duration and work week schedule does the PEL (0.75 ppm) for formaldehyde typically apply to?

<p>8 hours per day, 40-hour work week</p>
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What is the maximum time duration over which the STEL (2.0 ppm) for formaldehyde exposure is measured?

<p>15 minutes</p>
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What time duration is the Action Level (0.5 ppm) for formaldehyde exposure measured over?

<p>8 hours</p>
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True or False: If workplace exposure to formaldehyde exceeds any of the OSHA standard limits (PEL, STEL, or Action Level), modifications are required to lower employee exposure.

<p>True (A)</p>
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Formaldehyde itself exists in what state of matter?

<p>Gas</p>
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Formalin exists in what state of matter?

<p>Liquid</p>
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Paraformaldehyde exists in what state of matter?

<p>Solid</p>
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What is the name of the white solid substance that is known to collect at the bottom of formalin solutions?

<p>Paraformaldehyde</p>
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Acidity can promote the formation of paraformaldehyde. To counteract this, formalin solutions are typically buffered to what approximate pH range?

<p>7.0-9.0</p>
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Besides buffering, what common additive helps prevent the formation of paraformaldehyde in formalin solutions?

<p>Methanol</p>
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The concentration of commercial formaldehyde solutions is often expressed as 'Index'. What units does the Index typically represent?

<p>Grams of formaldehyde per 100 milliliters of solution (g/100mL)</p>
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How many grams of formaldehyde gas are present in 100 mL of an aqueous solution that has a 20 Index?

<p>20g</p>
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What is the typical formaldehyde index range for embalming fluids described as 'Low', often used for decedents with low moisture content?

<p>0-18</p>
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What is the typical formaldehyde index range for embalming fluids described as 'Medium', suitable for cases with normal moisture content?

<p>19-27</p>
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What is the typical formaldehyde index range for embalming fluids described as 'High', used for cases with high moisture content like edema?

<p>28-36</p>
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High Index formalin is considered _____ relative to body cells and is often used when dehydrating the tissue is desirable.

<p>Hypertonic</p>
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Low index formalin is considered _____ relative to body cells and is used when hydrating the tissues is desired.

<p>Hypotonic</p>
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Beyond moisture control, why might a high index formaldehyde solution be preferred in cases of advanced decomposition?

<p>Ammonia and urea (products of decomposition) can interfere with or neutralize formaldehyde.</p>
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What substance is formed from the reaction between ammonia and formaldehyde?

<p>Urotropin (also known as Methenamine or Hexamine)</p>
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What common household chemical is effective for neutralizing and cleaning up formaldehyde spills?

<p>Ammonia</p>
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What is the common name for the dialdehyde C5H8O2, often used in embalming and as a cold sterilant?

<p>Glutaraldehyde</p>
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What is the IUPAC (systematic) name for glutaraldehyde?

<p>Pentanedial</p>
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What are two advantages of using glutaraldehyde compared to formaldehyde in certain applications?

<p>It has a larger optimal pH window and does not shrink or constrict tissue as much as formaldehyde.</p>
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List some advantageous properties of Glutaraldehyde.

<p>More effective disinfectant, removes less water, disinfects faster at lower concentrations, creates more cross-linkages, better penetrating quality.</p>
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What is the molecular formula for Glutaraldehyde?

<p>C5H8O2</p>
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Glutaraldehyde solutions are known to be effective at killing which specific virus?

<p>HIV (Human Immunodeficiency Virus)</p>
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For disinfection purposes, how long should items typically be immersed in a 2% glutaraldehyde solution?

<p>20 minutes</p>
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For sterilization, what is the recommended range for immersion time in a 2% glutaraldehyde solution?

<p>6-10 hours</p>
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Sterilization achieved through immersion in glutaraldehyde solution at room temperature is known as what type of sterilization?

<p>Cold sterilization</p>
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What is a common method for achieving hot sterilization, often used for heat-resistant items?

<p>Autoclave</p>
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What specific immersion time in glutaraldehyde is sometimes cited as required for sterilization?

<p>10 hours</p>
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What minimum exposure time to glutaraldehyde is typically needed to effectively kill bacterial spores?

<p>3 hours</p>
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Before instruments are immersed in glutaraldehyde solution for disinfection or sterilization, what condition must they meet?

<p>They must be dry.</p>
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In chemical identification, what does the acronym CAS stand for?

<p>Chemical Abstracts Service</p>
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What is the primary function of the Chemical Abstracts Service (CAS)?

<p>To assign a unique identification number (CAS Registry Number) to every distinct chemical substance.</p>
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CAS began assigning unique numbers to chemical substances described in the scientific literature starting from what year?

<p>1957</p>
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Approximately how many unique organic/inorganic substances and protein/DNA sequences does the CAS Registry identify?

<p>Over 144 million organic and inorganic substances and 67 million protein and DNA sequences (numbers may increase over time).</p>
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When was formaldehyde discovered and when was it conclusively identified?

<p>Discovered in 1859 and conclusively identified in 1869.</p>
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Formaldehyde is a colorless toxic gas with a disagreeable odor. When dissolved in water, vapor is _____

<p>trapped</p>
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Formaldehyde is known to be quite reactive and its propensity to polymerize into ____________ was quite interesting.

<p>paraformaldehyde</p>
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Formaldehyde is sold as a _____ solution in water, stabilized by methanol.

<p>37%</p>
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Aqueous solutions of formaldehyde are commonly referred to as formalin. Chemically, in water, formaldehyde primarily exists as what compound?

<p>methylene glycol</p>
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Formaldehyde was thought first discovered by _____ in Russia, in 1859.

<p>Alexander Butlerov</p>
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Formaldehyde wasn't formally identified until 1869 at the University of Berlin by Professor _____.

<p>August Hoffman</p>
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In its basic form, what state of matter is formaldehyde?

<p>colorless gas</p>
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The most common concentration of formaldehyde in aqueous solution (formalin) is _____ by weight.

<p>37%</p>
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What is the common name for the liquid mixture of formaldehyde gas dissolved in water?

<p>Formalin</p>
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To prevent the polymerization of formaldehyde solution into paraformaldehyde, about _____ is added as a stabilizer.

<p>10 - 15% of methyl alcohol</p>
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It is the addition of _____ that causes the stabilized aqueous formaldehyde solution to be called formalin.

<p>methyl alcohol</p>
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What is the solid, polymerized form of formaldehyde called?

<p>Paraformaldehyde</p>
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Due to the health risks associated with formaldehyde, what monitoring does OSHA require in workplaces where it is used?

<p>Air Sampling</p>
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What is the OSHA Permissible Exposure Limit (PEL) for formaldehyde, averaged over an 8-hour Time Weighted Average (TWA)?

<p>0.75 ppm</p>
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What is the OSHA Short-Term Exposure Level (STEL) for formaldehyde, which should not be exceeded during any 15-minute period?

<p>2 ppm</p>
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What is the OSHA Action Level for formaldehyde, averaged over an 8-hour TWA, which triggers requirements like exposure monitoring and medical surveillance?

<p>0.5 ppm</p>
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In what year was the OSHA Formaldehyde Standard passed?

<p>1992</p>
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What is the time period over which the formaldehyde PEL of 0.75 ppm is averaged?

<p>8 hrs, 40 hr work week</p>
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What is the maximum time duration for exposure at the STEL of 2.0 ppm for formaldehyde?

<p>15 min</p>
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What is the time period over which the formaldehyde Action Level of 0.5 ppm is averaged?

<p>8 hrs.</p>
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True or False: If workplace exposure to formaldehyde exceeds any of the OSHA standard limits (PEL, STEL, Action Level), the employer must implement changes to reduce employee exposure.

<p>True (A)</p>
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In its pure form, formaldehyde is a _____

<p>gas</p>
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Formalin, the aqueous solution of formaldehyde, is a _____

<p>liquid</p>
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Paraformaldehyde, the polymerized form of formaldehyde, is a _____

<p>solid</p>
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_____ is a white solid that often collects on the bottom of formalin solutions due to polymerization.

<p>Paraformaldehyde</p>
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Since acidity promotes paraformaldehyde formation, formalin is typically buffered to an approximate pH range of _____.

<p>7.0-9.0</p>
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Besides buffering, _____ is added to formalin solutions to prevent paraformaldehyde formation.

<p>Methanol</p>
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Formaldehyde concentration in embalming fluids is often expressed by its Index. What does Index represent?

<p>grams/100 mLs</p>
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A formaldehyde solution with a 20 Index contains _____ grams of formaldehyde in 100 mLs of solution.

<p>20g</p>
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If an embalming fluid has a formaldehyde index of 20, how many grams of formaldehyde are present per 100 mL?

<p>20</p>
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What formaldehyde index range is typically considered 'Low', often used for decedents with low moisture content?

<p>0-18</p>
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What formaldehyde index range is typically considered 'Medium', suitable for cases with normal moisture content?

<p>19-27</p>
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What formaldehyde index range is considered 'High', often applied in cases with high moisture content (edema) or advanced decomposition?

<p>28-36</p>
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High Index fluids (_____ to the cells) are used when drawing moisture out of the tissues (dehydration) is desirable.

<p>Hypertonic</p>
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Low index fluids (_____ to the cells) may be used when adding moisture to or hydrating the tissues is desired.

<p>Hypotonic</p>
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Besides cases with high moisture, when else might a high index formaldehyde solution be particularly useful?

<p>During cases of advanced decomposition where ammonia and urea can interfere with the formaldehyde</p>
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What substance is formed when ammonia reacts with formaldehyde?

<p>Urotropin</p>
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What common household chemical can be used to neutralize spilled formaldehyde?

<p>Ammonia</p>
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What specific brand of ammonia was mentioned by Dr. Pugel in the context of formaldehyde neutralization?

<p>Little Bo Peep</p>
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Besides formaldehyde, what other aldehyde is commonly used in embalming fluids and as a cold sterilant?

<p>Glutaraldehyde</p>
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What is the IUPAC (Systematic) name for glutaraldehyde?

<p>Pentanedial</p>
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What are two advantages of using glutaraldehyde in embalming fluids compared to formaldehyde?

<p>Larger optimal pH window, Does not shrink or constrict tissue as much</p>
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List three properties or advantages of glutaraldehyde.

<p>More effective as a disinfectant, removes less water in the reaction, disinfects quicker at lower concentrations (other possible answers: creates more cross linkages, has better penetrating quality).</p>
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What is the molecular formula for Glutaraldehyde?

<p>C5H8O2</p>
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Glutaraldehyde is known to be effective at killing many microorganisms, including which notable virus?

<p>HIV</p>
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For disinfection purposes, how long should instruments typically be immersed in a 2% glutaraldehyde solution?

<p>20 min</p>
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For sterilization purposes, what is the typical immersion time required in a 2% glutaraldehyde solution?

<p>6-10 hrs</p>
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Sterilization using glutaraldehyde immersion at room temperature is referred to as what type of sterilization?

<p>cold sterilization</p>
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What is a common method used for hot (heat-based) sterilization?

<p>Autoclave</p>
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To achieve sterilization, approximately how many hours of immersion in glutaraldehyde are typically required?

<p>10 hrs.</p>
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How long an exposure time to glutaraldehyde is typically required to kill bacterial spores?

<p>3 hrs</p>
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If instruments only need to be disinfected (not sterilized), how long might cold sterilization with glutaraldehyde take according to some protocols?

<p>10 min</p>
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What must be done to instruments before they are immersed in glutaraldehyde solution for disinfection or sterilization?

<p>They must be Dry</p>
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What organization does the acronym CAS stand for?

<p>Chemical Abstracts Service</p>
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What is the primary function of the Chemical Abstracts Service (CAS)?

<p>Assign a unique number to every chemical substance</p>
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CAS has assigned unique numbers to chemical substances described in scientific literature covering what time period?

<p>1957-today</p>
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Approximately how many unique organic/inorganic substances and protein/DNA sequences does the CAS Number system identify?

<p>144 mil substances, 67 mil sequences</p>
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When was formaldehyde discovered and when was it conclusively identified?

<p>Discovered in 1859 and conclusively identified in 1869.</p>
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Formaldehyde is a colorless toxic gas with a disagreeable odor. When dissolved in water, vapor is _____.

<p>trapped</p>
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Formaldehyde is known to be quite reactive and its propensity to polymerize into __________ was quite interesting.

<p>paraformaldehyde</p>
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Formaldehyde is sold as a _____ solution in water, stabilized by methanol.

<p>37%</p>
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Aqueous solutions of formaldehyde are commonly referred to as formalin. What molecule is actually formed when formaldehyde dissolves in water?

<p>Methylene glycol</p>
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Who is thought to have first discovered formaldehyde in Russia in 1859?

<p>Alexander Butlerov</p>
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Who formally identified formaldehyde in 1869 at the University of Berlin?

<p>Professor August Hoffman</p>
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In its basic form, formaldehyde exists in what physical state?

<p>Colorless gas</p>
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The most common concentration of formaldehyde is a _____ solution.

<p>37%</p>
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What is the common name for the liquid mixture of formaldehyde gas and water?

<p>Formalin</p>
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To prevent polymerization of formaldehyde solution, about ____________________________ is added.

<p>10 - 15% of methyl alcohol</p>
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It is the addition of ___________ that causes the substance to be called formalin as opposed to formaldehyde.

<p>methyl alcohol</p>
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What is the solid form of formaldehyde called?

<p>Paraformaldehyde</p>
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What does OSHA require workplaces to perform when using formaldehyde?

<p>Air Sampling</p>
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What is the OSHA Permissible Exposure Limit (PEL) for formaldehyde over an 8-hour Time-Weighted Average (TWA)?

<p>0.75 ppm</p>
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What is the OSHA Short-Term Exposure Level (STEL) for formaldehyde, measured over a maximum 15-minute period?

<p>2 ppm</p>
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What is the OSHA Action Level for formaldehyde over an 8-hour TWA?

<p>0.5 ppm</p>
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In what year was the OSHA Formaldehyde Standard passed?

<p>1992</p>
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What is the time limit associated with the PEL of 0.75 ppm for formaldehyde exposure?

<p>8 hrs, 40 hr work week</p>
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What is the maximum time duration associated with the STEL of 2.0 ppm for formaldehyde exposure?

<p>15 min</p>
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What is the time limit associated with the Action Level of 0.5 ppm for formaldehyde exposure?

<p>8 hrs.</p>
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True or False: If exposure of formaldehyde exceeds any of the standard limits (PEL, STEL, Action Level), changes must be made to reduce employee exposure.

<p>True (A)</p>
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Formaldehyde is a _____.

<p>gas</p>
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Formalin is a _____.

<p>liquid</p>
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Paraformaldehyde is a _____.

<p>solid</p>
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__________ is a white solid that collects on the bottom of formalin solutions.

<p>Paraformaldehyde</p>
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Acidity promotes paraformaldehyde formation, and therefore formalin is buffered to approximately pH ________.

<p>7.0-9.0</p>
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__________ also prevents paraformaldehyde formation.

<p>Methanol</p>
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How is formaldehyde concentration sometimes expressed in terms of weight per volume, also known as Index?

<p>grams/100 mLs</p>
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A formaldehyde solution with a 20 Index contains _____g of formaldehyde in 100 mLs of solution.

<p>20g</p>
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If an embalming fluid has a formaldehyde index of 20, how many grams of formaldehyde are present in 100 mL?

<p>20</p>
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What is the typical formaldehyde index range for fluids described as 'Low', often used for low moisture decedents?

<p>0-18</p>
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What is the typical formaldehyde index range for fluids described as 'Medium', often used for normal moisture cases?

<p>19-27</p>
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What is the typical formaldehyde index range for fluids described as 'High', often used for high moisture (edematous) cases?

<p>28-36</p>
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High Index fluids (__________ to the cells) are used when dehydrating the tissue is desirable.

<p>Hypertonic</p>
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Low index fluids (__________ to the cells) are used when hydrating the tissues is desired.

<p>Hypotonic</p>
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__________ fluids are also used during cases of advanced decomposition where ammonia and urea can interfere with the formaldehyde.

<p>High index</p>
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What substance is formed when ammonia reacts with formaldehyde?

<p>Urotropin</p>
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What common household chemical can be used to neutralize and clean up formaldehyde spills?

<p>Ammonia</p>
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What specific brand of ammonia was mentioned by Dr. Pugel in relation to formaldehyde neutralization?

<p>Little Bo Peep</p>
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What dialdehyde chemical is commonly used in embalming and disinfection, besides formaldehyde?

<p>Glutaraldehyde</p>
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What is the IUPAC (systematic chemical) name for glutaraldehyde?

<p>Pentanedial</p>
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List two advantages of using glutaraldehyde compared to formaldehyde.

<p>Larger optimal pH window, Does not shrink or constrict tissue as much.</p>
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What chemical is considered more effective as a disinfectant, removes less water, disinfects quicker at lower concentrations, creates more cross-linkages, and penetrates better than formaldehyde?

<p>Glutaraldehyde</p>
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What is the molecular formula for Glutaraldehyde?

<p>C5H8O2</p>
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Glutaraldehyde is known to be effective against a wide range of microorganisms, including which specific virus mentioned?

<p>HIV</p>
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For chemical disinfection, how long should instruments typically be immersed in a 2% glutaraldehyde solution?

<p>20 min</p>
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For chemical sterilization, what is the typical immersion time required in a 2% glutaraldehyde solution?

<p>6-10 hrs</p>
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Immersion in glutaraldehyde solution for sterilization is considered what type of sterilization process?

<p>cold sterilization</p>
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What is a common apparatus used for 'hot' sterilization, using steam under pressure?

<p>Autoclave</p>
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According to one reference point, sterilization (killing all microbial life including spores) requires how many hours of immersion in glutaraldehyde?

<p>10 hrs.</p>
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How long should exposure time to glutaraldehyde be to effectively kill resistant bacterial spores?

<p>3 hrs</p>
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According to one reference point, if instruments need only disinfection (not sterilization), how long might cold sterilization with glutaraldehyde take?

<p>10 min</p>
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What condition must instruments be in before being immersed into glutaraldehyde solution for disinfection or sterilization?

<p>Dry</p>
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What does the acronym CAS stand for in the context of chemical identification?

<p>Chemical Abstracts Service</p>
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What is the main purpose of the Chemical Abstracts Service (CAS)?

<p>Assign a unique number to every chemical substance</p>
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CAS has assigned unique numbers to chemical substances described in the scientific literature starting from what year?

<p>1957</p>
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Approximately how many unique organic/inorganic substances and how many protein/DNA sequences does the CAS Registry identify?

<p>More than 144 million unique organic and inorganic substances and 67 million protein and DNA sequences</p>
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Flashcards

Formaldehyde

Discovered in 1859 and conclusively identified in 1869.

Trapped

Formaldehyde is a colorless toxic gas with a disagreeable odor. When dissolved in water, vapor is trapped.

Paraformaldehyde

Known to be quite reactive and its propensity to polymerize into paraformaldehyde was quite interesting.

37%

Formaldehyde is sold as a 37% solution in water, stabilized by methanol.

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Methylene glycol

Aqueous solutions of formaldehyde are commonly referred to as formalin because it's not actually formaldehyde...it's methylene glycol.

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Alexander Butlerov

Formaldehyde was thought first discovered by Alexander Butlerov in Russia, in 1859.

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August Hoffman

Formaldehyde wasn't formally identified until 1869 at the University of Berlin by Professor August Hoffman.

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Formaldehyde

In its basic form it is a colorless gas.

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37%

The most common concentration of formaldehyde is a 37% solution.

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Formalin

The liquid is actually a mixture of formaldehyde gas and water.

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10 - 15% of methyl alcohol

To prevent polymerization of formaldehyde solution, about 10 - 15% of methyl alcohol is added.

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Methyl alcohol

It is the addition of methyl alcohol that causes the substance to be called formalin as opposed to formaldehyde.

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Paraformaldehyde

Paraformaldehyde is the solid form of formaldehyde?

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Air Sampling

With the use of formaldehyde, OSHA requires air sampling.

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0.75 ppm

What is the (PEL) Permissible Exposure limit that OSHA allows for formaldehyde? (8 hour TWA) - 0.75 ppm

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2 ppm

What is the (STEL) Short term exposure level that OSHA allows for formaldehyde? (max. 15 minute period)- 2 ppm

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0.5 ppm

What is the Action Level that OSHA allows for formaldehyde? (8 hour TWA)- 0.5 ppm

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1992

When was the OSHA Formaldehyde Standard passed? 1992

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8 hrs, 40 hr work week

What is the PEL time limit for 0.75 ppm of formaldehyde? 8 hrs, 40 hr work week

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15 min

What is the STEL time limit for 2.0 ppm of formaldehyde? 15 min

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8 hrs.

What is the Action Level time limit with 0.5 ppm of formaldehyde? 8 hrs.

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TRUE

TRUE: If expose of formaldehyde exceeds any of the standard limits, changes must be made to reduce employee exposure.

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gas

Formaldehyde is a gas.

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liquid

Formalin is a liquid.

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solid

Paraformaldehyde is a solid.

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Paraformaldehyde

Paraformaldehyde is a white solid that collects on the bottom of formalin solutions

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7.0-9.0

Acidity promotes paraformaldehyde formation and therefore formalin is buffered to approximated 7.0-9.0

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Methanol

Methanol also prevents paraformaldehyde formation.

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grams/100 mLs

grams/100 mLs or more formally: Index

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20g

A formaldehyde solution with a 20 Index is 20 formaldehyde in 100 mLs of solution

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20

If the formaldehyde index is 20, what does that equal in grams?- 20

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0-18

Description is Low, Application is Low moisture decedent has what index range for formaldehyde? 0-18

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19-27

Description is medium, Application is Normal moisture. What would be the index range for formaldehyde? 19-27

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28-36

Description is High, Application is High moisture (edematous). What would be the index range for formaldehyde? 28-36

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Hypertonic

High Index (Hypertonic to the cells) is used when dehydrating the tissue is desirable.

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Hypotonic

Low index (Hypotonic to the cells) is used when hydrating the tissues is desired.

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High index

High index is also used during cases of advanced decomposition where ammonia and urea can interfere with the formaldehyde

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Urotropin

Ammonia + Formaldehyde = Urotropin

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Ammonia

If formaldehyde has been spilled, what do you use to clean it up? Ammonia

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Little Bo Peep

What brand of ammonia did Dr. Pugel mention? Little Bo Peep

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Glutraldehyde Structure

Chemical structure of Glutraldehyde

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Pentanedial

What is the IUPAC name for glutaraldehyde? Pentanedial

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Glutaraldehyde Advantages

Larger optimal pH window, Does not shrink or constrict tissue

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Glutaraldehyde Traits

*More effective as a disinfectant *removes less water in the reaction *disinfects quicker at lower concentrations *creates more cross linkages *has better penetrating quality

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C5H8O2

What is the molecular formula for Glutaraldehyde? C5H8O2

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HIV

What virus can Glutaraldehyde kill? HIV

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20 min

How long do you immerse in 2% glutaraldehyde for disinfection? 20 min

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6-10 hrs

How long do you immerse in 2% glutaraldehyde for sterilization? 6-10 hrs

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cold sterilization

Glutaraldehyde immersion is considered what type of sterilization? cold sterilization

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Autoclave

What is a way to do hot sterilization? Autoclave

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10 hrs.

Sterilization is how many hours of immersion in glutaraldehyde? 10 hrs.

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3 hrs

How long should exposure time to glutaraldehyde do you need to kill spores? 3 hrs

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10 min

If instruments need to be disinfected only, how long will it take cold sterilization to disinfect? 10 min

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Dry

What must insturments need to be before immersed into glutaraldehyde? Dry

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Chemical Abstracts Service

What is CAS? Chemical Abstracts Service

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CAS Purpose

Assign a unique number to every chemical substance

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1957-today

CAS has assigned a unique number to every chemical substance in defined literature starting in 1957 to today.

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144 mil, 67 mil

CAS Number identifies more than 144 mil unique organic and inorganic substances and 67 mil protein and DNA sequences

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Study Notes

  • Study notes on embalming chemistry

Formaldehyde

  • Discovered in 1859, conclusively identified in 1869.
  • It's a colorless, toxic gas with a disagreeable odor.
  • Vapor is trapped when dissolved in water.
  • Commonly sold as a 37% solution in water, stabilized by methanol.
  • Liquid solutions are commonly called formalin because they contain methylene glycol instead of formaldehyde.
  • First thought to be discovered by Alexander Butlerov in Russia in 1859.
  • Formally identified in 1869 by Professor August Hoffman at the University of Berlin.
  • In its basic form, it is a colorless gas.
  • Formalin is a mixture of formaldehyde gas and water.
  • About 10-15% of methyl alcohol is added to prevent polymerization of formaldehyde solution.
  • The addition of methyl alcohol causes the substance to be called formalin as opposed to formaldehyde.
  • Paraformaldehyde is the solid form of formaldehyde.
  • OSHA requires air sampling when using formaldehyde.
  • The Permissible Exposure Limit (PEL) that OSHA allows for formaldehyde is 0.75 ppm (8-hour TWA).
  • The Short Term Exposure Level (STEL) that OSHA allows for formaldehyde is 2 ppm (max. 15-minute period).
  • The Action Level that OSHA allows for formaldehyde is 0.5 ppm (8-hour TWA).
  • The OSHA Formaldehyde Standard was passed in 1992.
  • The PEL time limit for 0.75 ppm of formaldehyde is 8 hours within a 40-hour work week.
  • The STEL time limit for 2.0 ppm of formaldehyde is 15 minutes.
  • The Action Level time limit with 0.5 ppm of formaldehyde is 8 hours.
  • If exposure to formaldehyde exceeds any of the standard limits, changes must be made to reduce employee exposure.
  • Is a gas in it's basic form
  • Formalin is a liquid
  • Paraformaldehyde is a solid
  • Paraformaldehyde is a white solid that collects on the bottom of formalin solutions.
  • Acidity promotes paraformaldehyde formation, so formalin is buffered to approximately 7.0-9.0 pH.
  • Methanol also prevents paraformaldehyde formation.
  • Often sold by grams/100 mLs
  • A formaldehyde solution with a 20 Index is 20g formaldehyde in 100 mLs of solution.
  • If the formaldehyde index is 20, that equals 20 grams.
  • A low index range (0-18) would be most suitable for low moisture decedent cases.
  • A medium index range (19-27) would be most suitable for normal moisture cases.
  • A high index range (28-36) would be most suitable for high moisture (endemous) cases.
  • High Index (Hypertonic) is used when dehydrating the tissue is desirable.
  • Low index (Hypotonic) is used when hydrating the tissues is desired.
  • Is also used during cases of advanced decomposition where ammonia and urea can interfere with the formaldehyde
  • Ammonia + Formaldehyde = Urotropin
  • Ammonia is used to clean up formaldehyde spills.
  • "Little Bo Peep" is one brand of ammonia.

Glutaraldehyde

  • IUPAC name: Pentanedial
  • Has a larger optimal pH window, does not shrink or constrict tissue
  • More effective as a disinfectant, removes less water in the reaction, disinfects quicker at lower concentrations, creates more cross linkages, has better penetrating quality
  • Molecular formula is C5H8O2
  • Can kill HIV
  • 20-minute immersion in 2% glutaraldehyde for disinfection.
  • 6-10 hour immersion in 2% glutaraldehyde for sterilization.
  • Glutaraldehyde immersion is considered cold sterilization.
  • Autoclaving is a way to do hot sterilization.
  • Sterilization is achieved through 10 hours of immersion in glutaraldehyde
  • A 3-hour exposure to glutaraldehyde is needed to kill spores.
  • If instruments need only disinfection, it will take 10 minutes of cold sterilization to disinfect.
  • Instruments must be dry before immersion into glutaraldehyde.

CAS

  • Stands for Chemical Abstracts Service.
  • Assigns a unique number to every chemical substance.
  • CAS has assigned a unique number to every chemical substance in defined literature starting in 1957 to today.
  • CAS Number identifies more than 144 million unique organic and inorganic substances and 67 million protein and DNA sequences.

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