ELISA Technique and Mabs Applications Quiz
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Questions and Answers

What is the main concept of the ELISA technique?

  • Visualizing proteins separated by SDS-PAGE
  • Measuring hormone values in serum and/or plasma sample
  • Using antibodies and color change to identify a substance (correct)
  • Detecting blood group types
  • What is the purpose of using Monoclonal Antibodies in ELISA?

  • To measure hormone values in serum and/or plasma sample
  • To visualize proteins separated by SDS-PAGE
  • To identify blood group types
  • To detect antigens/protein molecules in biological samples (correct)
  • Which diagnostic technique is used to visualize proteins separated by SDS-PAGE?

  • Immunoblot (Western blot) (correct)
  • ELISA
  • Blood group test
  • None of the above
  • What does ELISA rely on monoclonal antibodies to detect?

    <p>Antigens/protein molecules in biological samples</p> Signup and view all the answers

    Study Notes

    ELISA Technique

    • The main concept of ELISA (Enzyme-Linked Immunosorbent Assay) is to detect and quantify antibodies or antigens in a sample.

    Monoclonal Antibodies in ELISA

    • The purpose of using monoclonal antibodies in ELISA is to provide high specificity and sensitivity in detecting specific antigens or antibodies.

    Diagnostic Technique

    • Western Blot (Immunoblot) is a diagnostic technique used to visualize proteins separated by SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis).

    ELISA Detection

    • ELISA relies on monoclonal antibodies to detect specific antigens or antibodies, allowing for precise identification and quantification.

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    Description

    Test your knowledge on the main concepts and types of ELISA, as well as the diagnostic applications of Monoclonal Antibodies (Mabs) in clinical settings. Explore the use of ELISA in identifying substances and its significance in diagnostics.

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