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Questions and Answers
What is the main concept of the ELISA technique?
What is the main concept of the ELISA technique?
- Visualizing proteins separated by SDS-PAGE
- Measuring hormone values in serum and/or plasma sample
- Using antibodies and color change to identify a substance (correct)
- Detecting blood group types
What is the purpose of using Monoclonal Antibodies in ELISA?
What is the purpose of using Monoclonal Antibodies in ELISA?
- To measure hormone values in serum and/or plasma sample
- To visualize proteins separated by SDS-PAGE
- To identify blood group types
- To detect antigens/protein molecules in biological samples (correct)
Which diagnostic technique is used to visualize proteins separated by SDS-PAGE?
Which diagnostic technique is used to visualize proteins separated by SDS-PAGE?
- Immunoblot (Western blot) (correct)
- ELISA
- Blood group test
- None of the above
What does ELISA rely on monoclonal antibodies to detect?
What does ELISA rely on monoclonal antibodies to detect?
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Study Notes
ELISA Technique
- The main concept of ELISA (Enzyme-Linked Immunosorbent Assay) is to detect and quantify antibodies or antigens in a sample.
Monoclonal Antibodies in ELISA
- The purpose of using monoclonal antibodies in ELISA is to provide high specificity and sensitivity in detecting specific antigens or antibodies.
Diagnostic Technique
- Western Blot (Immunoblot) is a diagnostic technique used to visualize proteins separated by SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis).
ELISA Detection
- ELISA relies on monoclonal antibodies to detect specific antigens or antibodies, allowing for precise identification and quantification.
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