Podcast
Questions and Answers
What is the purpose of moistening a strip of filter paper with buffer in electrophoresis?
What is the purpose of moistening a strip of filter paper with buffer in electrophoresis?
- To help the samples migrate according to their charges (correct)
- To prevent the samples from moving
- To make the gel more rigid
- To speed up the process of gel casting
Why are certain compounds such as proteins and hydrophilic molecules difficult to resolve in paper electrophoresis?
Why are certain compounds such as proteins and hydrophilic molecules difficult to resolve in paper electrophoresis?
- Due to the lack of electrodes in the process
- Due to the adsorptive and ionogenic properties of paper (correct)
- Because these compounds are too small
- Because paper electrophoresis does not use a buffer
Which type of gel serves as a molecular sieve in gel electrophoresis?
Which type of gel serves as a molecular sieve in gel electrophoresis?
- Polyacrylamide
- Starch
- Sodium chloride
- Agarose (correct)
What type of material is agarose, commonly used in gel electrophoresis?
What type of material is agarose, commonly used in gel electrophoresis?
How are samples recovered in agarose gel electrophoresis?
How are samples recovered in agarose gel electrophoresis?
What is the main advantage of using agarose gels compared to other matrices in gel electrophoresis?
What is the main advantage of using agarose gels compared to other matrices in gel electrophoresis?
What is the primary use of agarose gels in electrophoresis?
What is the primary use of agarose gels in electrophoresis?
What is the basic repeat unit of agarose?
What is the basic repeat unit of agarose?
Why are low percentage agarose gels weak?
Why are low percentage agarose gels weak?
Which method is used for separating proteins ranging in size from 5 to 2,000 kDa?
Which method is used for separating proteins ranging in size from 5 to 2,000 kDa?
Why is it important to control acrylamide concentrations when creating polyacrylamide gels?
Why is it important to control acrylamide concentrations when creating polyacrylamide gels?
Which type of gels are used most often in the field of immunology and protein analysis?
Which type of gels are used most often in the field of immunology and protein analysis?
