12 Questions
What is the purpose of moistening a strip of filter paper with buffer in electrophoresis?
To help the samples migrate according to their charges
Why are certain compounds such as proteins and hydrophilic molecules difficult to resolve in paper electrophoresis?
Due to the adsorptive and ionogenic properties of paper
Which type of gel serves as a molecular sieve in gel electrophoresis?
Agarose
What type of material is agarose, commonly used in gel electrophoresis?
Natural colloid/polysaccharide polymer
How are samples recovered in agarose gel electrophoresis?
Easily
What is the main advantage of using agarose gels compared to other matrices in gel electrophoresis?
Ease of casting and handling
What is the primary use of agarose gels in electrophoresis?
Separating very large molecules with a molecular mass greater than 200 kDa
What is the basic repeat unit of agarose?
Agarobiose
Why are low percentage agarose gels weak?
They may break easily
Which method is used for separating proteins ranging in size from 5 to 2,000 kDa?
Polyacrylamide gel electrophoresis (PAGE)
Why is it important to control acrylamide concentrations when creating polyacrylamide gels?
To modulate the pore size of the gel
Which type of gels are used most often in the field of immunology and protein analysis?
Polyacrylamide gels
Explore the technique used for separating small charged molecules such as amino acids and small proteins. Learn about how a strip of filter paper immersed in buffer solutions with electrodes helps in visualizing the separation process.
Make Your Own Quizzes and Flashcards
Convert your notes into interactive study material.
Get started for free
