Electrophoresis Technique for Small Molecules
12 Questions
0 Views

Choose a study mode

Play Quiz
Study Flashcards
Spaced Repetition
Chat to lesson

Podcast

Play an AI-generated podcast conversation about this lesson

Questions and Answers

What is the purpose of moistening a strip of filter paper with buffer in electrophoresis?

  • To help the samples migrate according to their charges (correct)
  • To prevent the samples from moving
  • To make the gel more rigid
  • To speed up the process of gel casting
  • Why are certain compounds such as proteins and hydrophilic molecules difficult to resolve in paper electrophoresis?

  • Due to the lack of electrodes in the process
  • Due to the adsorptive and ionogenic properties of paper (correct)
  • Because these compounds are too small
  • Because paper electrophoresis does not use a buffer
  • Which type of gel serves as a molecular sieve in gel electrophoresis?

  • Polyacrylamide
  • Starch
  • Sodium chloride
  • Agarose (correct)
  • What type of material is agarose, commonly used in gel electrophoresis?

    <p>Natural colloid/polysaccharide polymer</p> Signup and view all the answers

    How are samples recovered in agarose gel electrophoresis?

    <p>Easily</p> Signup and view all the answers

    What is the main advantage of using agarose gels compared to other matrices in gel electrophoresis?

    <p>Ease of casting and handling</p> Signup and view all the answers

    What is the primary use of agarose gels in electrophoresis?

    <p>Separating very large molecules with a molecular mass greater than 200 kDa</p> Signup and view all the answers

    What is the basic repeat unit of agarose?

    <p>Agarobiose</p> Signup and view all the answers

    Why are low percentage agarose gels weak?

    <p>They may break easily</p> Signup and view all the answers

    Which method is used for separating proteins ranging in size from 5 to 2,000 kDa?

    <p>Polyacrylamide gel electrophoresis (PAGE)</p> Signup and view all the answers

    Why is it important to control acrylamide concentrations when creating polyacrylamide gels?

    <p>To modulate the pore size of the gel</p> Signup and view all the answers

    Which type of gels are used most often in the field of immunology and protein analysis?

    <p>Polyacrylamide gels</p> Signup and view all the answers

    More Like This

    Use Quizgecko on...
    Browser
    Browser