Electrophoresis: Introduction and Definition

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In moving boundary electrophoresis, the electrophoresis is done in a ______ solution

free

Zone electrophoresis can be performed on a solid support like filter paper or ____

gel

____ electrophoresis involves the migration of charged particles on supporting media

Zone

Isotachophoresis is a type of ______ electrophoresis

capillary

Electrophoresis is useful for the separation of small charged molecules like amino acids and small ____

proteins

Electrophoresis is a physical method of analysis which involves separation of the compounds that are capable of acquiring electric charge. Electrophoresis may be defined as the migration of the charged particle through a solution under the influence of an external electrical field. Ions that are suspended between two electrodes tend to travel towards the electrodes that bear ______ charges.

opposite

Electrophoresis (Electro refers to the energy of electricity and Phoresis, from the Greek verb phoros, means to carry across) is a technique for separating or resolving charged molecules (such as amino acids, peptides, proteins, nucleotides, and nucleic acids) in a mixture under the influence of an applied electric _____.

field

Charged molecules in an electric field move or migrate, at a speed determined by their charge to mass _____.

ratio

According to the laws of electrostatics, an ion with charge ‘Q’ in an electric field of strength ‘E’ will experience an electric force, Felectrical = Q.E The resulting migration of the charged molecule through the solution is opposed by a frictional force Ffrictional = V.f where, V is the rate of migration of charged molecule and f is its frictional _____.

coefficient

Frictional coefficient depends on the size and shape of the migrating molecule and the ______ of the medium.

viscosity

Learn about the physical method of analysis involving separation of compounds with electric charge. Understand the migration of charged particles through a solution under an external electrical field and the movement towards opposite charged electrodes.

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