Electrophoresis of Serum Proteins
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Questions and Answers

Which protein band exhibits the highest migration toward the anode?

  • α1-globulin
  • Albumin (correct)
  • γ-globulin
  • β-globulin

Which reference range correctly matches the protein band α2-globulin?

  • 5-9 g/L (correct)
  • 6-11 g/L
  • 2-4 g/L
  • 7-17 g/L

Which proteins are predominantly found in the γ-globulin band?

  • Transferrin and plasminogen
  • Immunoglobulins (correct)
  • α1-antitrypsin and HDL
  • Haptoglobin and α2-macroglobulin

Using plasma instead of serum in protein electrophoresis introduces an additional band of which protein?

<p>Fibrinogen (D)</p> Signup and view all the answers

Which protein band does not contain a mixture of proteins?

<p>Albumin (D)</p> Signup and view all the answers

What is the correct reference range for the β-globulin band?

<p>6-11 g/L (A)</p> Signup and view all the answers

Study Notes

Normal SPE (Serum Protein Electrophoresis)

  • Electrophoresis separation of serum protein mixture on cellulose acetate shows five bands, in order of highest to lowest migration toward the anode:

Bands and Reference Ranges

  • Albumin: 35-52 g/L
  • α1-globulin: 2-4 g/L
  • α2-globulin: 5-9 g/L
  • β-globulin: 6-11 g/L
  • γ-globulin: 7-17 g/L

Composition of Each Band (Except Albumin)

  • α1-globulin: contains α1-antitrypsin, α1-acid glycoprotein, α1-fetoprotein, HDL, and others
  • α2-globulin: contains α2-macroglobulin, haptoglobin, and others
  • β-globulin: contains transferrin, β2-microglobulin, plasminogen, LDL, and others
  • γ-globulin: contains mainly immunoglobulins (IgG, IgA, IgM, and to a lesser extent IgD and IgE)

Important Note

  • If plasma is used instead of serum, an additional fibrinogen band appears between β and γ-globulin bands, which can be confused with the M-band, so serum should be used in protein electrophoresis.

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Description

This quiz covers the separation of serum proteins through electrophoresis on cellulose acetate, including the five bands that form and their respective reference ranges.

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