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Questions and Answers
Which protein band exhibits the highest migration toward the anode?
Which protein band exhibits the highest migration toward the anode?
- α1-globulin
- Albumin (correct)
- γ-globulin
- β-globulin
Which reference range correctly matches the protein band α2-globulin?
Which reference range correctly matches the protein band α2-globulin?
- 5-9 g/L (correct)
- 6-11 g/L
- 2-4 g/L
- 7-17 g/L
Which proteins are predominantly found in the γ-globulin band?
Which proteins are predominantly found in the γ-globulin band?
- Transferrin and plasminogen
- Immunoglobulins (correct)
- α1-antitrypsin and HDL
- Haptoglobin and α2-macroglobulin
Using plasma instead of serum in protein electrophoresis introduces an additional band of which protein?
Using plasma instead of serum in protein electrophoresis introduces an additional band of which protein?
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Which protein band does not contain a mixture of proteins?
Which protein band does not contain a mixture of proteins?
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What is the correct reference range for the β-globulin band?
What is the correct reference range for the β-globulin band?
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Study Notes
Normal SPE (Serum Protein Electrophoresis)
- Electrophoresis separation of serum protein mixture on cellulose acetate shows five bands, in order of highest to lowest migration toward the anode:
Bands and Reference Ranges
- Albumin: 35-52 g/L
- α1-globulin: 2-4 g/L
- α2-globulin: 5-9 g/L
- β-globulin: 6-11 g/L
- γ-globulin: 7-17 g/L
Composition of Each Band (Except Albumin)
- α1-globulin: contains α1-antitrypsin, α1-acid glycoprotein, α1-fetoprotein, HDL, and others
- α2-globulin: contains α2-macroglobulin, haptoglobin, and others
- β-globulin: contains transferrin, β2-microglobulin, plasminogen, LDL, and others
- γ-globulin: contains mainly immunoglobulins (IgG, IgA, IgM, and to a lesser extent IgD and IgE)
Important Note
- If plasma is used instead of serum, an additional fibrinogen band appears between β and γ-globulin bands, which can be confused with the M-band, so serum should be used in protein electrophoresis.
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