Electron Microscopy: TEM and SEM
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Questions and Answers

Which of the following characteristics differentiates a Transmission Electron Microscope (TEM) from a Scanning Electron Microscope (SEM)?

  • TEM requires specimen sectioning and staining, whereas SEM does not. (correct)
  • TEM utilizes light waves, whereas SEM uses electron beams.
  • TEM scans the surface of an object, whereas SEM penetrates the object.
  • TEM provides three-dimensional images, whereas SEM provides two-dimensional images.

An archaeologist discovers a preserved insect and needs to examine its surface structures in high detail. Which type of microscopy would be most suitable?

  • Scanning Electron Microscopy (SEM) (correct)
  • Light Microscopy
  • Transmission Electron Microscopy (TEM)
  • Fluorescence Microscopy

A researcher is studying the internal structure of a virus. Which microscopy technique would be most appropriate for visualizing these details?

  • Transmission Electron Microscopy (TEM) (correct)
  • Dark-field Microscopy
  • Scanning Electron Microscopy (SEM)
  • Phase-contrast Microscopy

If a scientist aims to create a high-resolution, three-dimensional image of the exterior of a bacterial cell, which microscopy method should they employ?

<p>Scanning Electron Microscopy (SEM) (C)</p> Signup and view all the answers

Which of the following describes a key limitation of Transmission Electron Microscopy (TEM) compared to Scanning Electron Microscopy (SEM)?

<p>TEM requires extensive sample preparation, potentially altering the specimen. (D)</p> Signup and view all the answers

Flashcards

Electron Microscopes

Microscopes using electrons as an energy source for high magnification.

TEM (Transmission Electron Microscope)

Electron microscope where electron beams pass through a specimen creating 2D images, useful for internal details.

TEM Specimen Preparation

Involves specific steps to prepare specimens for TEM, like cutting into thin slices and adding contrast.

SEM (Scanning Electron Microscope)

Electron microscope that scans the surface of an object to create 3D images.

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SEM: No Sectioning

Type of microscopy that avoids cutting specimens, preserving the natural surface structure.

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Study Notes

  • Electron microscopes utilize electrons as their energy source.

Transmission Electron Microscope (TEM)

  • Electron beams pass through the specimen.
  • Requires special preparation involving sectioning and staining.
  • Produces two-dimensional images.
  • Provides good internal detail of specimens.

Scanning Electron Microscope (SEM)

  • Does not require sectioning of the specimen.
  • Scans the surface of an object.
  • Creates a three-dimensional image.
  • An example is the Scanning Electron Micrograph of Campylobacter.

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Description

Learn about electron microscopes, which use electrons as an energy source. The transmission electron microscope (TEM) transmits electron beams through a specimen. The scanning electron microscope (SEM) scans the surface of an object to create three-dimensional images.

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