EasySep Direct Immunomagnetic Isolation Quiz

EasySep Direct is used for isolating cells directly from whole blood without the need for RBC lysis, density gradient centrifugation, or other pre-processing steps.

EDTA must be added when isolating myeloid cells.

The RapidSpheres should be vortexed for 30 seconds.

<p>The isolation cocktail should be added using a pipette.</p> Signup and view all the answers

<p>The sample should be mixed using a pipette due to the viscous nature of blood.</p> Signup and view all the answers

<p>The sample should be incubated at room temperature according to the product information sheet, typically for 5 minutes.</p> Signup and view all the answers

<p>For lymphoid isolations, calcium and magnesium free PBS should be used to top up the sample.</p> Signup and view all the answers

<p>The sample should be mixed using a pipette by drawing the sample up and down 2-3 times.</p> Signup and view all the answers

<p>The tube should be placed into the magnet without a lid, ensuring it is fully seated in the indentation.</p> Signup and view all the answers

<p>The final step is not provided in the text.</p> Signup and view all the answers

<p>To keep the magnetically labelled unwanted cells &amp; RBCs to the back &amp; bottom of the tube.</p> Signup and view all the answers

<p>It must be pipetted off, not poured.</p> Signup and view all the answers

<p>2mL pipette should be used.</p> Signup and view all the answers

<p>It can be placed at a 45 degree angle.</p> Signup and view all the answers

<p>Collect the upper fraction of clear cell suspension, taking care not to disturb particles on the magnet side of the tube.</p> Signup and view all the answers

<p>Use a fixed collection volume and refer to the info sheet for volume of cell suspension to be collected.</p> Signup and view all the answers

<p>Collect entire upper phase from top to bottom moving down the front of the tube, and also collect a small volume of RBC pellet (up to 10% of original sample volume).</p> Signup and view all the answers

<p>RapidSpheres should be added.</p> Signup and view all the answers

<p>Mix with a pipette.</p> Signup and view all the answers

<p>The enriched cell suspension should be transferred to a new tube.</p> Signup and view all the answers

<p>To keep the magnetically labeled unwanted cells and RBCs to the back and bottom of the tube, allowing the clear supernatant containing enriched cell suspension to be pipetted off.</p> Signup and view all the answers

<p>A fixed collection volume with some RBCs to ensure optimal recovery, as indicated in the information sheet.</p> Signup and view all the answers

<p>RapidSpheres should be added to the new tube containing the enriched cell suspension.</p> Signup and view all the answers

<p>Collect the entire upper phase from top to bottom moving down the front of the tube, and also collect a small volume of RBC pellet (up to 10% of the original sample volume).</p> Signup and view all the answers

<p>The mixture should be mixed with a pipette.</p> Signup and view all the answers

<p>Pipette the supernatant off the bottom RBC pellet, collecting the upper fraction of clear cell suspension from top to bottom, taking care not to disturb particles on the magnet side of the tube.</p> Signup and view all the answers

<p>The enriched cell suspension should be transferred to a new tube and is now ready for use.</p> Signup and view all the answers

<p>The tube should have the lid removed and be placed into the EasySep magnet for a second round of separation.</p> Signup and view all the answers

<p>The text does not specify a particular duration for vortexing the RapidSpheres.</p> Signup and view all the answers

<p>The sample should be mixed with a pipette.</p> Signup and view all the answers

<p>EasySep Direct is used for isolating cells directly from whole blood without the need for RBC lysis, density gradient centrifugation, or other pre-processing steps.</p> Signup and view all the answers

<p>If isolating myeloid cells, EDTA must be added to the round bottom tube.</p> Signup and view all the answers

<p>The RapidSpheres should be vortexed for 30 seconds.</p> Signup and view all the answers

<p>After adding the isolation cocktail to the sample, the RapidSpheres should be added to the sample using a pipette.</p> Signup and view all the answers

<p>Using a pipette is important when mixing the sample because blood is viscous, and using a pipette ensures it is well mixed.</p> Signup and view all the answers

<p>The sample should be incubated at room temperature, with timing according to the product information sheet, typically around 5 minutes.</p> Signup and view all the answers

<p>The sample should be topped up with the recommended medium. For lymphoid isolations, calcium and magnesium free PBS should be used, while for myeloid isolations, calcium and magnesium free PBS with 1 mM EDTA should be used.</p> Signup and view all the answers

<p>After topping up the sample with the recommended medium, it should be mixed using a pipette by drawing the sample up and down 2-3 times.</p> Signup and view all the answers

<p>Before placing the tube into the magnet, the lid should be removed, and the tube should be seated fully in the indentation.</p> Signup and view all the answers

<p>The purpose of the EasySep Direct platform is to provide a fast, column-free, fully immunomagnetic platform for isolating cells directly from whole blood without the need for RBC lysis, density gradient centrifugation, or other pre-processing steps.</p> Signup and view all the answers