Podcast
Questions and Answers
What is EasySep Direct used for?
What is EasySep Direct used for?
EasySep Direct is used for isolating cells directly from whole blood without the need for RBC lysis, density gradient centrifugation, or other pre-processing steps.
What must be added to the whole blood when isolating myeloid cells?
What must be added to the whole blood when isolating myeloid cells?
EDTA must be added when isolating myeloid cells.
How long should the RapidSpheres be vortexed for?
How long should the RapidSpheres be vortexed for?
The RapidSpheres should be vortexed for 30 seconds.
How should the isolation cocktail be added to the sample?
How should the isolation cocktail be added to the sample?
What should be done after adding the RapidSpheres to the sample?
What should be done after adding the RapidSpheres to the sample?
How long should the sample be incubated at room temperature?
How long should the sample be incubated at room temperature?
What medium should be used to top up the sample for lymphoid isolations?
What medium should be used to top up the sample for lymphoid isolations?
How should the sample be mixed after topping up with the recommended medium?
How should the sample be mixed after topping up with the recommended medium?
Where should the tube be placed after mixing the sample?
Where should the tube be placed after mixing the sample?
What is the final step after placing the tube into the magnet?
What is the final step after placing the tube into the magnet?
What is the purpose of incubating the sample at room temperature on the magnet?
What is the purpose of incubating the sample at room temperature on the magnet?
What should be done with the clear supernatant containing enriched cell suspension?
What should be done with the clear supernatant containing enriched cell suspension?
What volume of pipette should be used for the 5mL side of the magnet?
What volume of pipette should be used for the 5mL side of the magnet?
How should the EasyEights magnet be placed for easier visualization?
How should the EasyEights magnet be placed for easier visualization?
What should be done when pipetting the supernatant off the bottom RBC pellet?
What should be done when pipetting the supernatant off the bottom RBC pellet?
What should be done if isolating myeloid cells?
What should be done if isolating myeloid cells?
What should be done if isolating lymphoid cells?
What should be done if isolating lymphoid cells?
What should be added to the new tube containing the enriched cell suspension?
What should be added to the new tube containing the enriched cell suspension?
What should be done after adding RapidSpheres to the new tube?
What should be done after adding RapidSpheres to the new tube?
What is the final step to prepare enriched cells for use?
What is the final step to prepare enriched cells for use?
What is the purpose of incubating the sample at room temperature on the magnet?
What is the purpose of incubating the sample at room temperature on the magnet?
What medium should be used to top up the sample for myeloid isolations?
What medium should be used to top up the sample for myeloid isolations?
What should be added to the new tube containing the enriched cell suspension?
What should be added to the new tube containing the enriched cell suspension?
What should be done if isolating lymphoid cells?
What should be done if isolating lymphoid cells?
What should be done after adding RapidSpheres to the new tube?
What should be done after adding RapidSpheres to the new tube?
What should be done when pipetting the supernatant off the bottom RBC pellet?
What should be done when pipetting the supernatant off the bottom RBC pellet?
What is the final step to prepare enriched cells for use?
What is the final step to prepare enriched cells for use?
Where should the tube be placed after mixing the sample?
Where should the tube be placed after mixing the sample?
How long should the RapidSpheres be vortexed for?
How long should the RapidSpheres be vortexed for?
How should the sample be mixed after topping up with the recommended medium?
How should the sample be mixed after topping up with the recommended medium?
What is EasySep Direct used for?
What is EasySep Direct used for?
What needs to be added to the round bottom tube if isolating myeloid cells?
What needs to be added to the round bottom tube if isolating myeloid cells?
How long should the RapidSpheres be vortexed for?
How long should the RapidSpheres be vortexed for?
What should be done after adding the isolation cocktail to the sample?
What should be done after adding the isolation cocktail to the sample?
Why is using a pipette important when mixing the sample?
Why is using a pipette important when mixing the sample?
How long should the sample be incubated at room temperature?
How long should the sample be incubated at room temperature?
What should be used to top up the sample, and what are the specific recommendations for lymphoid and myeloid isolations?
What should be used to top up the sample, and what are the specific recommendations for lymphoid and myeloid isolations?
How should the sample be mixed after topping it up with the recommended medium?
How should the sample be mixed after topping it up with the recommended medium?
What should be done before placing the tube into the magnet?
What should be done before placing the tube into the magnet?
What is the purpose of the EasySep Direct platform for isolating cells?
What is the purpose of the EasySep Direct platform for isolating cells?
