Lect. 7.

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Questions and Answers

Which enzyme cleaves double-stranded siRNA into smaller fragments?

  • Dicer (correct)
  • Intron
  • Argonaut
  • Ribonuclease

What is the function of the RISC complex in RNA interference?

  • Activating gene expression
  • Degradation of siRNA
  • Pairing with mRNA to inhibit translation or cleaving of the mRNA (correct)
  • Promoting mRNA stability

In RNA interference, what is used for gene knockdown in research?

  • Single-stranded RNA
  • Double-stranded RNA (correct)
  • Non-coding RNA
  • Micro RNA

What is the role of the antisense RNA in the formation of the RISC complex?

<p>Forms RISC complex with Argonaut to pair with mRNA (C)</p> Signup and view all the answers

What is the role of tryptophan in the regulation of the trp operon?

<p>Tryptophan acts as a co-repressor, binding to the inactive repressor and making it active to prevent transcription of the trp operon genes (D)</p> Signup and view all the answers

What is the consequence of an excess of tryptophan in the environment or in the cell?

<p>Transcription of the trp operon genes is limited, saving the cell energy and resources (C)</p> Signup and view all the answers

True or false: Tryptophan acts as a co-repressor in the regulation of the trp operon by preventing transcription of the structural genes.

<p>True (A)</p> Signup and view all the answers

True or false: When there is an excess of tryptophan in the environment, the trp operon is repressed, leading to limited transcription of the structural genes involved in tryptophan synthesis.

<p>True (A)</p> Signup and view all the answers

RNA silencing limits the transcript level by activating a sequence-specific RNA degradation process.

<p>True (A)</p> Signup and view all the answers

In the Lac operon, LacY encodes for transacetylase.

<p>False (B)</p> Signup and view all the answers

TCS is a type of regulatory system used by bacteria to detect chemical and/or physical signals found in its environment.

<p>True (A)</p> Signup and view all the answers

SiRNA is a type of RNA interference that involves short exogenous interfering dsRNA.

<p>True (A)</p> Signup and view all the answers

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