Dry Heat Sterilization of Glassware Preparation
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Questions and Answers

What should be done with conical flasks during the preparation for dry heat sterilization?

Plug the mouth with clean cotton wool and cover the plugs with Aluminum foil.

How should pipettes be prepared for dry heat sterilization?

Plug the mouth with cotton wool and burn the cotton fibers sticking out using a Bunsen flame. Wrap the pipettes individually in Aluminum foil or paper or store in a suitable container.

How should you prepare glassware for dry heat sterilization?

Wash glassware, clean and wipe dry thoroughly. Wrap the glassware in Aluminum foil or wrap in a paper or store in a suitable container.

How should you prepare the glassware for autoclaving?

<p>Prepare the glassware according to the outlined procedure, and plug the containers with cotton wool and cover with Aluminum foil or paper.</p> Signup and view all the answers

What temperature should the oven be set at for dry heat sterilization, and for how long?

<p>170°C for 1-2 hrs, depending on the amount of glassware in the oven.</p> Signup and view all the answers

What should be done with bottles with screw caps during autoclaving?

<p>Loosen the screw cap slightly.</p> Signup and view all the answers

How long should the autoclave run for sterilization?

<p>15 - 20 minutes at 121°C</p> Signup and view all the answers

What should be done with the valve of the autoclave during autoclaving?

<p>Open the valve.</p> Signup and view all the answers

How should containers be plugged before placing them in the autoclave?

<p>Plug the containers with cotton wool and cover with Aluminum foil or paper.</p> Signup and view all the answers

What should be done with bottles with screw caps during autoclaving?

<p>Loosen the screw cap slightly.</p> Signup and view all the answers

How long should the autoclave run for sterilization?

<p>15 - 20 minutes at 121°C</p> Signup and view all the answers

What type of heat is used for sterilization in an autoclave?

<p>Wet heat</p> Signup and view all the answers

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