DNA to Protein: Reading Genomes

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Questions and Answers

Which of the following is NOT a characteristic of RNA?

  • Contains uracil
  • Double-stranded structure (correct)
  • Contains ribose sugar
  • Often single-stranded

DNA synthesis is the intermediate for proteins.

False (B)

What type of bonds do DNA and RNA polymerase catalyze to connect the backbone?

phosphodiester bonds

During transcription, RNA polymerase links __________ to a growing mRNA chain.

<p>ribonucleoside triphosphates</p> Signup and view all the answers

Match the following terms with their descriptions:

<p>Transcription = Process of synthesizing RNA from a DNA template Translation = Process of synthesizing proteins from mRNA Template Strand = DNA strand used as a guide for RNA synthesis Coding Strand = DNA strand with the same sequence as the RNA transcript (except T instead of U)</p> Signup and view all the answers

What is the primary difference in building blocks used by RNA and DNA polymerase?

<p>RNA polymerase uses ribonucleotide triphosphates, while DNA polymerase uses deoxyribonucleotides. (D)</p> Signup and view all the answers

DNA polymerase requires primers to initiate synthesis.

<p>False (B)</p> Signup and view all the answers

According to the material, what is the ultimate purpose of RNA synthesis?

<p>intermediate for proteins</p> Signup and view all the answers

During transcription, once the DNA is open, one strand acts as the __________ for RNA synthesis.

<p>template</p> Signup and view all the answers

Which of the following is a key difference between DNA replication and transcription?

<p>DNA replication is not H-bonded, and the entire RNA transcript is moved, allowing DNA to form a helix while transcription is. (B)</p> Signup and view all the answers

Both DNA and RNA use the nitrogenous base Thymine.

<p>False (B)</p> Signup and view all the answers

What is the type of sugar found in RNA?

<p>ribose</p> Signup and view all the answers

Many __________ can be derived from one gene in transcription.

<p>RNA</p> Signup and view all the answers

Which of the following is a function of RNA?

<p>Intermediate step in protein synthesis. (A)</p> Signup and view all the answers

Both DNA and RNA contain the same number of strands.

<p>False (B)</p> Signup and view all the answers

Besides the sugar, what other base is different between DNA and RNA?

<p>uracil vs thymine</p> Signup and view all the answers

DNA contains the base ________, whereas RNA contains the base uracil.

<p>thymine</p> Signup and view all the answers

What is a common structural feature of both DNA and RNA?

<p>Both are composed of nucleotides. (C)</p> Signup and view all the answers

Both DNA and RNA use phosphodiester bonds to link nucleotides.

<p>True (A)</p> Signup and view all the answers

Why does DNA polymerase work without proofreading?

<p>doesn't req primers</p> Signup and view all the answers

Flashcards

Transcription

The process of copying a gene's DNA sequence to make an RNA molecule.

Translation

The process where genetic code in mRNA is decoded to produce a specific sequence of amino acids in a polypeptide chain

RNA characteristics

Ribose, uracil, often single-stranded, requires DNA to replicate and has unique shapes.

RNA Polymerase's Role

The enzyme that synthesizes RNA from a DNA template during transcription.

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Transcription vs DNA Replication

RNA is not hydrogen-bonded allowing DNA to form a helix. RNA transcripts are moved and are shorter.

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RNA Polymerase

Use ribonucleotide triphosphates to catalyze the links between ribonucleotides. Requires primers and works without proofreading.

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DNA characteristics

Deoxyribose, thymine, double-stranded, self-replicating

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DNA to RNA

Many RNA can be derived from one gene in transcription. It is ultimately the intermediate for proteins.

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DNA and RNA Polymerase

They catalyze phosphodiester bonds that connect the backbone.

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Study Notes

  • Chapter 7 focuses on DNA to protein: Reading Genomes.

DNA and RNA

  • Many RNA strands can be derived from one gene during transcription.
  • RNA synthesis serves as an intermediate step for protein creation.

Differences between DNA and RNA

  • RNA features include ribose, uracil, is often single-stranded, needs DNA to replicate, and has unique shapes.
  • DNA features deoxyribose, thymine, is double-stranded, and self-replicating.
  • Each has differing sugars, bases, number of strands, and replication needs.
  • Both are linear, use phosphodiester bonds, and use the bases A, C, and G.

Transcription and RNA Production

  • Once DNA is open, one strand acts as the template for RNA synthesis.
  • RNA polymerase links ribonucleoside triphosphates to a growing RNA chain.
  • The resulting RNA transcript is complementary to the original DNA.

DNA Replication vs. Transcription

  • RNA is not hydrogen-bonded, and the entire RNA transcript is moved, allowing DNA to form a helix.
  • RNA strands are also much shorter, approximately a couple thousand nucleotides long.
  • Both DNA and RNA polymerase catalyze phosphodiester bonds that connect the backbone.

RNA Polymerase vs. DNA Polymerase

  • RNA polymerase uses ribonucleotide triphosphates to catalyze links between ribonucleotides.
  • DNA polymerase works with deoxyribonucleotides.
  • RNA requires primers and does not have proofreading capabilities.

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